Oil biodegradation and bioremediation: A tale of the two worst spills in U.S. history

The devastating environmental impacts of the Exxon Valdez spill in 1989 and its media notoriety made it a frequent comparison to the BP Deepwater Horizon spill in the popular press in 2010, even though the nature of the two spills and the environments impacted were vastly different. Fortunately, unlike higher organisms that are adversely impacted by oil spills, microorganisms are able to consume petroleum hydrocarbons. These oil degrading indigenous microorganisms played a significant role in reducing the overall environmental impact of both the Exxon Valdez and BP Deepwater Horizon oil spills

Bacterial Populations in the Beaufort Sea

Spatial and seasonal variation patterns were studied for bacterial populations in Beaufort Sea ice, water and sediment. Bacterial populations in the Beaufort Sea were found in concentrations as high as are found in temperate oceans. Bacterial populations, especially viable bacteria, were lower in surface water during winter than summer, but not in sediment. Beaufort Sea bacterial populations are mainly psychrotrophic and are clearly adapted to growth at low temperatures. Ice conditions appear to be important in determining levels of bacterial populations in water

Hydrocarbons and Microbial Activities in Sediment of an Arctic Lake One Year After Contamination with Leaded Gasoline

Hydrocarbons were found to persist in the sediment of an Arctic lake one year after the lake was accidentally contaminated with leaded gasoline. The contaminating gasoline was continuing to spread from the original site of contamination. High numbers of hydrocarbon utilizing microorganisms were found in the contaminated sediment. Rates of nitrogen fixation did not appear to be affected by hydrocarbon contamination, but potential denitrification activities appeared to be altered by the gasoline. Fertilizer application resulted in a moderate decrease of hydrocarbon concentrations in the sediment

Long Term Interactions of Microorganisms and Prudhoe Bay Crude Oil in Tundra Soils at Barrow, Alaska

Oil was recovered from tundra soils two and seven years after spillage. Oil persisted in the upper soil layer. The depth of penetration appears to depend on soil moisture and drainage characteristics. Maximal penetration seems to occur within one year of spillage. Biodegradation of the oil was indicated by changes in the ratio of gas chromatographically resolved to unresolved components. Individual components appear to be preferentially degraded, but no evidence was found for significant preferential degradation of structural classes of hydrocarbons. Numbers of microorganisms were different in oil contaminated and reference soils generally showing continued enrichment, but in some soils showing inhibition of microbial populations

Fate of Crude and Refined Oils in North Slope Soils

Prudhoe Bay crude oil and refined diesel fuel were applied to five topographically distinct tundra soils at Prudhoe Bay, Alaska. The penetration of hydrocarbons into the soil column depended on soil moisture and drainage characteristics. Biodegradation, shown by changes in the pristane to heptadecane and resolvable to total gas chromatographic area ratios, appeared to be greatly restricted in drier tundra soils during one year exposure. Some light hydrocarbons, C9-C10, were recovered from soils one year after spillages. Hydrocarbons were still present in soils at Fish Creek, Alaska, contaminated by refined oil spillages 28 years earlier, attesting to the persistence of hydrocarbons in North Slope soils

Distribution of Hydrocarbons and Microbial Populations Related to Sedimentation Processes in Lower Cook Inlet and Norton Sound, Alaska

In spring and summer 1978 and spring 1979 an integrated study was carried out to examine the interrelationships of physical (sediment deposition), chemical (organic carbon and hydrocarbon concentrations), and biological (microbial populations and activities) factors in the Cook Inlet and Norton Sound regions with respect to the probable sinks and fates of hydrocarbon contaminants within these ecosystems. Most of the fine-grained sediment entering Cook Inlet is transported out of the inlet into Shelikof Strait. However, significant sediment accumulation occurs within areas of Kamishak and Kachemak bays. In Norton Sound, sediment from the Yukon River is transported counterclockwise around the embayment and approximately 50% is deposited in the nearshore regions of the sound. In both regions, areas of high sediment accumulation are richer in organic carbon and hydrocarbon derived from land than are areas of low sediment accumulation. In general, areas with high sediment accumulation rates for fine-grained particles are also areas of relatively high microbial activity. Results suggest that these elevated microbial activities reflect biodegradation of detrital carbon associated with these particles. Also, the Cook Inlet and Norton Sound region were found to be free from petroleum hydrocarbon contamination (with the exception of one area in Cook Inlet). No evidence was found of hydrocarbon accumulation resulting from a gas seepage in Norton Sound, nor for accumulation of hydrocarbons in sediments of lower Cook Inlet and Shelikof Strait from oil well operations in upper Cook Inlet.Key words: arctic marine ecosystems, sedimentation, microorganism, hydrocarbons, lower Cook Inlet, Norton SoundMots clés: écosystèmes marins arctiques, sédimentation, micro-organismes, hydrocarbons, sud de l'inlet Cook, bras de mer Norto

Oil spill problems and sustainable response strategies through new technologies

Crude oil and petroleum products are widespread water and soil pollutants resulting from marine and terrestrial spillages. International statistics of oil spill sizes for all incidents indicate that the majority of oil spills are small (less than 7 tonnes). The major accidents that happen in the oil industry contribute only a small fraction of the total oil which enters the environment. However, the nature of accidental releases is that they highly pollute small areas and have the potential to devastate the biota locally. There are several routes by which oil can get back to humans from accidental spills, e.g. through accumulation in fish and shellfish, through consumption of contaminated groundwater. Although advances have been made in the prevention of accidents, this does not apply in all countries, and by the random nature of oil spill events, total prevention is not feasible. Therefore, considerable world-wide effort has gone into strategies for minimising accidental spills and the design of new remedial technologies. This paper summarizes new knowledge as well as research and technology gaps essential for developing appropriate decision-making tools in actual spill scenarios. Since oil exploration is being driven into deeper waters and more remote, fragile environments, the risk of future accidents becomes much higher. The innovative safety and accident prevention approaches summarized in this paper are currently important for a range of stakeholders, including the oil industry, the scientific community and the public. Ultimately an integrated approach to prevention and remediation that accelerates an early warning protocol in the event of a spill would get the most appropriate technology selected and implemented as early as possible-the first few hours after a spill are crucial to the outcome of the remedial effort. A particular focus is made on bioremediation as environmentally harmless, cost-effective and relatively inexpensive technology. Greater penetration into the remedial technologies market depends on the harmonization of environment legislation and the application of modern laboratory techniques, e.g. ecogenomics, to improve the predictability of bioremediation

Global emissions of refrigerants HCFC-22 and HFC-134a: Unforeseen seasonal contributions

HCFC-22 (CHClF[subscript 2]) and HFC-134a (CH[subscript 2]FCF[subscript 3]) are two major gases currently used worldwide in domestic and commercial refrigeration and air conditioning. HCFC-22 contributes to stratospheric ozone depletion, and both species are potent greenhouse gases. In this work, we study in situ observations of HCFC-22 and HFC-134a taken from research aircraft over the Pacific Ocean in a 3-y span [HIaper-Pole-to-Pole Observations (HIPPO) 2009–2011] and combine these data with long-term ground observations from global surface sites [National Oceanic and Atmospheric Administration (NOAA) and Advanced Global Atmospheric Gases Experiment (AGAGE) networks]. We find the global annual emissions of HCFC-22 and HFC-134a have increased substantially over the past two decades. Emissions of HFC-134a are consistently higher compared with the United Nations Framework Convention on Climate Change (UNFCCC) inventory since 2000, by 60% more in recent years (2009–2012). Apart from these decadal emission constraints, we also quantify recent seasonal emission patterns showing that summertime emissions of HCFC-22 and HFC-134a are two to three times higher than wintertime emissions. This unforeseen large seasonal variation indicates that unaccounted mechanisms controlling refrigerant gas emissions are missing in the existing inventory estimates. Possible mechanisms enhancing refrigerant losses in summer are (i) higher vapor pressure in the sealed compartment of the system at summer high temperatures and (ii) more frequent use and service of refrigerators and air conditioners in summer months. Our results suggest that engineering (e.g., better temperature/vibration-resistant system sealing and new system design of more compact/efficient components) and regulatory (e.g., reinforcing system service regulations) steps to improve containment of these gases from working devices could effectively reduce their release to the atmosphere

Effectiveness of percutaneous laser disc decompression versus conventional open discectomy in the treatment of lumbar disc herniation; design of a prospective randomized controlled trial

Background. The usual surgical treatment of refractory sciatica caused by lumbar disc herniation, is open discectomy. Minimally invasive procedures, including percutaneous therapies under local anesthesia, are increasingly gaining attention. One of these treatments is Percutaneous Laser Disc Decompression (PLDD). This treatment can be carried out in an outpatient setting and swift recovery and return to daily routine are suggested. Thus far, no randomized trial into cost-effectiveness of PLDD versus standard surgical procedure has been performed. We present the design of a randomized controlled trial, studying the cost-effectiveness of PLDD versus conventional open discectomy in patients with sciatica from lumbar disc herniation. Methods/design. The study is a randomized prospective multi-center trial, in which two treatment strategies are compared in a parallel group design. Patients (age 18-70 years) visiting the neurosurgery department of the participating hospitals, are considered for inclusion in the trial when sciatica due to lumbar disc herniation has lasted more than 8 weeks. Patients with disc herniation smaller than 1/3 of the spinal canal diameter, without concomitant lateral recess stenosis or sequestration, are eligible for participation, and are randomized into one of two treatment arms; either Percutaneous Laser Disc Decompression or conventional discectomy. The functional outcome of the patient, as assessed by the Roland Disability Questionnaire for Sciatica at 8 weeks and 1 year after treatment, is the primary outcome measure. The secondary outcome parameters are recovery as perceived by the patient, leg and back pain, incidence of re-intervention, complications, quality of life, medical consumption, absence of work and secondary costs. Discussion. Open discectomy is still considered to be the golden standard in the surgical treatment of lumbar disc herniation. Whether Percutaneous Laser Disc Decompression has at least as much efficacy as the standard surgical procedure, and is more cost-effective, will be determined by this trial. Trial registration. Current Controlled Trials ISRCTN25884790