Map of Indonesia showing the island of Sumatera and indicating the sampling locations.

<p>Map of Indonesia showing the island of Sumatera and indicating the sampling locations.</p

Luminex serology on seventy-one¹ sera collected from Kota Medan and Deli Serdang.

<p>Sera with an MFI over 1000 were defined as strongly positive (denoted with a <u>solid </u>underline). Sera with an MFI over 200 were defined as potentially positive (denoted with a <u>dashed</u> underline).</p>1<p>Two samples were unsuitable for testing.</p

Conventional RT-PCR gel (M = 100 bp markers, 1 = UB21 undiluted, 2 = UB21 1∶10 dilution, 3 = PU21 undiluted, 4 = PU21 1∶10 dilution, 5 = DS21 undiluted, 6 = DS21 1∶10 dilution, 7 = PU18 undiluted, 8 = PU18 1∶10 dilution, 9 = PU20 undiluted, 10 = PU20 1∶10 dilution, 11 = Positive control, 12 = No template control).

<p>Conventional RT-PCR gel (M = 100 bp markers, 1 = UB21 undiluted, 2 = UB21 1∶10 dilution, 3 = PU21 undiluted, 4 = PU21 1∶10 dilution, 5 = DS21 undiluted, 6 = DS21 1∶10 dilution, 7 = PU18 undiluted, 8 = PU18 1∶10 dilution, 9 = PU20 undiluted, 10 = PU20 1∶10 dilution, 11 = Positive control, 12 = No template control).</p

Realtime PCR results in <i>P. vampyrus</i> samples from North Sumatera, 2009¹.

1<p>A fifth sample (pooled urine sample PU21) yielded a ‘trace’ result by real-time PCR but was negative by conventional PCR, and is not included here.</p

Alignment of a 251 nt sequence region of the Nipah virus matrix (M) gene from our Indonesian bat (P. vampyrus/2013/Genbank KC903168-KC903172), the AAHL reference virus (Malaysia/human/1999/Genbank AF212302), a Malaysian bat (P. vampyrus/2010/Genbank FN869553), Bangladesh (human/2004/Genbank AY988601) and India (human/2007/Genbank FJ513078).

<p>Alignment of a 251 nt sequence region of the Nipah virus matrix (M) gene from our Indonesian bat (P. vampyrus/2013/Genbank KC903168-KC903172), the AAHL reference virus (Malaysia/human/1999/Genbank AF212302), a Malaysian bat (P. vampyrus/2010/Genbank FN869553), Bangladesh (human/2004/Genbank AY988601) and India (human/2007/Genbank FJ513078).</p

Additional file 1 of Molecular characterization of recombinant LSDV isolates from 2022 outbreak in Indonesia through phylogenetic networks and whole-genome SNP-based analysis

Supplementary Material