Traceability environmental effects of microfabric in leaves of Cupressus dupreziana plant and Soil Surrounding it given the rise in COVID19

Background:The high death rate from COVID-19, which can increase mineral pollution in cemetery soils and necessitates future cemetery projects that are able to counteract these environmental effects, highlights the significance of studying metal concentrations in cemetery soils. The research analyzed the quantities of minerals in the cemetery soil in Najaf Governorate, Iraq, taking into account the high mortality during the sampling period in order to identify future projects for cemeteries targeted at mitigating environmental consequences.Methods: Using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM), the researchers investigated the leaf features of the soils in three cemeteries. soil samples were taken three times from a depth of 10 to 30 cm Cupressus dupreziana plants considering the analysis in triplicate with averaging, analytical deviation and used in the autoregressive integrated moving averages model, this curve had 50% traceability to bioavailability to reduce contamination risk.Results: The findings revealed elevated amounts of iron and lead (Pb) (Fe), tomb soil analysis. Take into account the rise in fatalities and the associated funerals. According to COVID-19, the estimated daily mortality toll is between (20-50); however, metal pollution may be more severe. In order to lessen the effects on the environment, the facility was expressed as a gas and effluent processor at 80% in soil of the proportion of the curve in the spatial analysis for mineral contamination.Conclusion: The SEM and EDX can serve as reliable indicators, while the morphometric approach offers a holistic understanding of the soil and its associated plant life by analyzing elemental compositions. Employing these methods allows for quantitative assessment and comparison against the control, aiding in the thorough characterization of Cupressus dupreziana. Furthermore, these analyses shed light on the various organism clusters found within the study area's soil

Analytical quality by design-based RP-HPLC method for quantification of pioglitazone and candesartan cilexetil in bilayer tablet and its forced degradation studies

Aim: The current project involves developing an RP-HPLC method for simultaneous quantification of Candesartan Cilexetil and Pioglitazone based on analytical quality by design (AQbD). Materials and methods: When analysed in the Design Expert application, the critical method parameters were systematically refined using Central Composite Design and contours were derived for significant variables. A contour plot has been used to discover the technique operable design region that governs response variation, which is then empirically tested. Results: Successful chromatographic separation of title analytes was achieved on kromasil C18 (150 × 4.6 mm, 5 µm) column at 30 °C with mobile phase comprising 60% 20 Mm Potassium dihydrogen orthophosphate and 40% acetonitrile (v/v), isocratic elution pattern, 0.9 mL/min flow rate, and UV detection at 220 nm. The linear model for Candesartan Cilexetil was from 4 to 24 µg/ mL and Pioglitazone at 7.5–45 µg/ mL, respectively. Conclusion: The method met all the ICH Q2 (R1) validation criteria. The current approach aided for analysing simultaneous drugs can be expanded into quantifying drugs in biological matrix predominance with maximum recovery