Genetıc Characterızatıon Of Pepper (Capsicum Annuum L.) Genotypes From Central Anatolıa Wıth Ssr And Scar Markers

The major objective in pepper breeding programs is to generate high yielding novel varieties resistant to pests and diseases, tolerant to abiotic stress conditions with improved fruit quality traits including capsaicin content. Germplasm collections are important sources of variability for breeding studies. Molecular markers are important tools to evaluate genetic relationships among germplasm collections. Moreover, markers are used to select the genotypes conferring the desired traits via marker-assisted selection (MAS) as a powerful approach accelerating breeding programs. In the current study, 56 pepper genotypes selected among 313 pepper genotypes collected from Kırşehir province in the Central Anatolian region of Turkey according to their agronomic and morphological characteristics were used for molecular assays. Six SSR markers two of which were linked to fruit morphology were selected to characterize pepper genotypes according to their high polymorphism information content. Three SCAR markers associated with capsaicinoid synthesis and resistance to Phytophtora capsici (Phyto.5.2) in pepper were used to assess pungency and resistance among genotypes. According to the results obtained with SSR markers, the total number of alleles ranged from 1 to 8 among genotypes. The most polymorphic SSR markers were CaeMS015 and CAMS452 within the pepper population. The genetic distance among genotypes was determined ranging between 0.75-1.00. The segregation of the SCAR marker BF6-BF8 linked to pungency in pepper was determined relative to pungent and sweet reference cultivars. The OP004.717 SCAR marker linked to Phytophtora capsici was tested among genotypes relative to CM334 pepper variety, known as a source of resistance to Phytophtora. © 2022, Genetika. All Rights Reserved

Effects of pacemaker and implantable cardioverter defibrillator electrodes on tricuspid regurgitation and right sided heart functions

Background: The aim of this study was to assess the effect of trans-tricuspid placement of permanent pacemaker (PPM), implantable cardioverter defibrillator (ICD) and cardiac resynchronization therapy (CRT) leads prospectively on tricuspid valve and right-sided heart functions using two-dimensional echocardiography. Methods: A total of 41 patients (31 male, mean age: 63.6 ± 12.2 years) were included in this prospective study. Initial echocardiographic evaluation was performed before cardiac device implantation and re-evaluation by echocardiography was performed immediately after the procedure and at 1st, 6th and 12th months. In addition to standard echocardiographic examinations, vena contracta (VC), proximal isovelocity surface area (PISA), and tissue Doppler evaluations were also performed in the study population. Results: Tricuspid regurgitation (TR) is worsened by 1 grade in 70.8% of the patients and 2 grades in 17.1% of the patients in the follow-up. Eight patients without baseline TR developed new-onset TR (9.8% mild, 9.8% moderate) after lead implantation. In the follow-up period, 41.5% of the patients who had mild TR before lead implantation developed moderate TR and 7.3% developed severe TR, whereas 19.5% of the patients with moderate TR developed severe TR during the follow-up. In the follow-up period, VC of TR was increased [median: 0.32 (0.16–0.60) cm in pre-implantation period, and 0.41 (0.18–0.80) cm at 12th month, p = 0.001]. Similarly PISA value of TR was also increased [median: 0.46 (0.15–1.10) cm in pre-implantation period and 0.52 (0.28–1.20) cm at 12th month, p = 0.001]. However, there is not a significant difference between PPMs/ICDs and CRTs regarding the effects on TR (p < 0.05). In addition, right ventricular dimensions and right atrial volumes were increased during the follow-up. Conclusions: Implantation of permanent transvenous right ventricular electrode is associated with worsening of TR, right atrial and right ventricular dimensions. Further studies are needed in order to both outline the effect of those findings on outcomes and clarify the time dependent changes in those functions

The Moderating Role of Job Satisfaction on the Relationship between Servant Leadership and Turnover Intention

One of the aims of this study is to determine how the servant leadership will affect turnover intention and job satisfaction in tourism sector. Another aim is to discover whether job satisfaction has an indirect effect on the relationship between servant leadership and turnover intention. The data used in this study is gathered through survey in November 2016, from 191 participants employed in accommodation facilities in Belek-Antalya. Cronbach’s Alpha coefficients of the scales used in the survey are calculated and they are proved to be rather reliable for this sample. The results of regression analyses show that there is a positive relationship between servant leadership and job satisfaction however there is a negative relationship between servant leadership and turnover intention. There is a negative relationship between job satisfaction and turnover intention and there is a partial moderating role of job satisfaction on the relationship between servant leadership and turnover intention

Plasma Chemerin Levels Are Increased in ST Elevation Myocardial Infarction Patients with High Thrombus Burden

Objective. To investigate plasma chemerin levels in ST elevation myocardial infarction (STEMI) patients and find out possible relationships between plasma chemerin levels and angiographic characteristics. Patients and Methods. Ninety-seven consecutive patients who presented with STEMI and underwent primary percutaneous coronary intervention (PCI) with coronary stents were enrolled, and 30 age- and sex-matched patients with stable angina pectoris who underwent coronary angiography formed the control group. Angiographic characteristics of the patients including thrombolysis in myocardial infarction (TIMI) thrombus and Gensini scores were noted. Blood samples were taken to detect several biochemical markers including plasma chemerin levels at the admission to hospital. Results. Serum chemerin and C-reactive protein (CRP) levels were significantly increased in patients with STEMI. Among STEMI patients, serum chemerin levels were significantly higher in patients with high thrombus burden (581.5 ± 173.7 versus 451.3 ± 101.2 mg/dL, p<0.001). CRP levels and peak creatine kinase-MB (CK-MB) levels were higher, and left ventricular ejection fraction and post-PCI TIMI flow were lower in patients with high thrombus burden. After multivariate analysis, serum chemerin levels were also higher in patients with high thrombus grade (odds ratio: 1.009 (1.005–1.014), p<0.001). Besides, serum chemerin levels were also found to be significantly correlated with CRP r=0.47,p<0.001 and peak CK-MB r=0.376,p<0.001 levels. Conclusions. Results from our study have demonstrated for the first time that chemerin levels were higher in STEMI patients with greater thrombus burden and higher level of inflammation

Complications of Robotic Surgery in Urology: Our Experience of 342 Procedures Including the Learning Curve

Objective:The aim of this study is to analyze the complications of all robot-assisted surgeries performed in our clinic and to investigate the effect of the learning curve on complications occurred.Materials and Methods:Data on a total of 342 robotic surgeries performed in our clinic between March 2015 and February 2018 was retrospectively analyzed. Two surgeons, who performed the surgery, were experienced in urological laparoscopic procedures. Intraoperative and postoperative complications were evaluated. According to the experience of robotic surgery, the complications were divided into two groups as those occurred in the first 18 months (March 2015-August 2016) and in the second 18 months (September 2016-February 2018). The complications were classified according to the Clavien-Dindo classification.Results:A total of 32 complications occurred in 31 of 342 patients undergoing robot-assisted surgery. The overall complication rate was 9.4%. The number of minor complications was 20 (62.5%), and the number of major complications was 12 (37.5%). Among all, 6.2% were intraoperative complications, 62.5% were postoperative complications, and 31.3% were medical complications. It was observed that the number of complications was plateaued after August 2016 and there was a statistically significant difference between the first and the second 18 months (p<0.05).Conclusion:Laparoscopic surgery experience alone is not enough to reduce complications in robot-assisted surgery and learning continues with every case. An experienced surgeon and robotic surgery team are needed for complex robotic surgeries

Aggressive Treatment of Refractory Coronary Artery Vasospasm in a Patient with Malignant Ventricular Tachyarrhythmia and Cardiac Arrest

Coronary artery vasospasm (CAVS) is a clinical entity that can cause angina, but also unstable angina pectoris, acute myocardial infarction, fatal arrhythmias, and sudden death. Although it is a condition that is usually controlled with medical treatment, more aggressive treatments may rarely be required. In this case, the patient with a known diagnosis of CAVS had multiple arrests despite optimal medical treatment. We observed that fatal arrhythmias persisted in the Implantable Cardioverter Defibrillator (ICD) records, even though we implanted a stent and gave the patient maximal medical treatment. We performed sympathectomy as a last resort and we did not detect any recurrence in the 6-month follow-up of the patient. ICD implantation and sympathectomy should always be considered in resistant CAVS cases

Using complementary and alternative medicine in patients with breast cancer diagnosis at Afyon Kocatepe University

AMAÇ: Bu çalışmada Afyon Kocatepe Üniversitesi’nde meme kanseri nedeniyle takip edilmekte olan hastalarda tamamlayıcı ve alternatif tedavi (TAT) kullanım sıklığının ve TAT ile ilgili uygulamaların belirlenmesi ve incelenmesi amaçlanmıştır. GEREÇ VE YÖNTEM: Çalışmamıza Afyon Kocatepe Üniversitesi Tıp Fakültesi Hastanesi, Tıbbi Onkoloji Polikliniği ile Fizik Tedavi ve Rehabilitasyon Ana Bilim Dalı’na başvuran toplam 70 meme kanserli hasta dahil edilmiştir. Hastaların sosoyodemografik özellikleri ile, kullanılan TAT yöntemlerine yönelik sorgulamayı içeren 44 soruluk bir form doldurulmuştur. BULGULAR: Ortalama yaşları 57.5 ± 11.2 yıl olan hastaların en az bir TAT yöntemi kullanma oranı %85.7 (60 kişi) olarak belirlenmiştir. TAT yöntemi olarak en çok dua etmenin (%85.7) ve bitkilerin (%30) tercih edildiği saptanmıştır. TAT yöntemi kullanan meme kanserli hastaların %55’i TAT kullanmaya kendisi karar verirken, %13.3’ü ailesinin etkisiyle TAT kullanmaya başlamış. Çalışmamızda TAT kullanan hastaların sadece %35’inin bu konuda doktoruna bilgi verdiği görülmüştür. TAT kullanan hastaların %32.9’u çok fayda gördüğünü belirtirken, bu hastaların %44.3’ü tanı aldıktan hemen sonra tedavi süreci başlamadan bu yöntemlere başvurduğunu ifade etmiştir. SONUÇ: Hastalarımızın TAT yöntemlerini sık olarak kullandıkları, çoğunlukla da dua ve bitkisel yöntemleri tercih ettikleri saptanmıştır. Ayrıca meme kanserli kadınların TAT kullanımı ile ilgili sağlık çalışanlarına bilgi vermediği, özellikle kemoterapi, radyoterapi/ hormon tedavisi devam edenlerin büyük çoğunluğunun beraberinde TAT kullandığı belirlenmiştir. Ayrıca; sağlık çalışanlarının hastaları TAT kullanma yönünden sorgulamaları, böylelikle bu yöntemlerin kullanımı ile ilgili oluşabilecek yan etkilerin önlenmesine de yardımcı olunabileceğini düşünmekteyiz.OBJECTIVE: We aimed to determine the frequency of complementary and alternative therapy (CAM) usage and CAM related applications in patients with breast cancer at Afyon Kocatepe University. MATERIAL AND METHODS: Our study included a total of 70 patients with breast cancer who applied to the Afyon Kocatepe University Medical Faculty Hospital, Medical Oncology and Physical Medicine and Rehabilitation outpatient clinics. A 44-question form including questionnaires on the sociodemographic characteristics of the patients and the CAM methods used were filled out. RESULTS: Patients with a mean age of 57.5 ± 11.2 years were assigned at least one CAM method as 85.7% (60 people). It was determined that; praying (85.7%) and plants (30%) were as the most preferred CAM method. While 55% of breast cancer patients using the CAM method decided to use oneself, 13.3% of them started to use CAM due to the influence of their family. In our study, only 35% of the patients using CAM were informed about this issue by their doctor. While 32.9% of patients using CAM indicated that they were very beneficial, 44.3% of the patients stated that they applied to these methods immediately after the diagnosis was started. CONCLUSIONS: It has been determined that our patients use CAM methods frequently, mostly they prefer prayer and herbal methods. In addition, breast cancer patients do not inform health workers about the use of CAM, especially the majority of those continuing with chemotherapy, radiotherapy / hormone therapy were using CAM together. As a result; we also think that health professionals can also interrogate patients in terms of using CAM so that they can also help prevent side effects from using these methods

Saanen tekesinde rete mirabile epidurale rostrale’nin morfolojik görünümü

The rostral epidural rete mirabile in small ruminants is bilaterally located next to the hypophysis in the cranial cavity, lies in the cavernous sinus, and regulates arterial blood flow of the brain. In this study, structural peculiarity of the rostral epidural rete mirabile in the ten male mature Saanen goats (11 months old, approximately 42 kg in weight, with a known pedigree, and obtained from Bolu region) was examined by corrosion cast technique using Takilon. Dissections have demonstrated that general architecture of the rostral epidural rete mirabile in the Saanen goat agrees with that in other small ruminants. It is structured by the branches from the arteria maxillaris only. The triangular shaped right and left components (the right and left parts) anastomose, forming a rostrally V-shaped structure. This anastomose was not observed in one of the materials. Thus, gross views and diameters of the retial arteries were not markedly different on the left and right sides. The maxillary artery, the rostral and caudal rami, and the rostral epidural rete mirabile were visualized by the corrosion cast technique. The caudal epidural rete mirabile and the chiasmatic rete were observed to lack in the Saanen goat.Rete mirabile epidurale rostrale küçük ruminantlarda cavum cranii içinde, hipofiz bezinin her iki yanında, sinus cavernosus içinde yer alan ve beynin arteriel kan akışını düzenleyen bir yapıdır. Bu çalışmada, Takilon ile korozyon kast tekniği kullanılarak, Bolu bölgesinden temin edilmiş 10 adet Saanen tekesinde (11 aylık, yaklaşık 42 kg ağırlığında ve şecereli) rete mirabile epidurale rostrale’nin yapısal özellikleri gözlemlenmiştir. Yapılan diseksiyonda bu anatomik yapının, genel özellikleri itibariyle diğer küçük ruminant türlerinde gözlemlenen yapı ile benzerlik gösterdiği belirlenmiştir. Bu atardamar ağının sadece arteria maxillaris’ten köken alan dallar tarafından oluşturulduğu ortaya konmuştur. Sağ ve sol tarafta yer alan ve üçgen görünümlü olan ağ bölümlerinin (pars dextra ve pars sinistra) ağızlaşarak ön tarafta ‘’V’’ şeklinde bir yapı oluşturduğu gözlemlenmiştir. Fakat bu ağızlaşma bir bireyde görülmemiştir. Ayrıca, atardamar ağının sağ ve sol yarımının makroskopik görünümleri arasında bir farklılık bulunmamıştır. Arteria maxillaris, ramus caudalis ad rete mirabile epidurale rostrale, rami rostrales ad rete mirabile epidurale rostrale adlı dallar korozyon kast tekniği ile net bir şekilde ortaya çıkartılmıştır. Yine, diseke edilen hayvanlarda rete mirabile epidurale caudale ve rete chiasmaticum’un şekillenmediği ortaya konulmuştur