12 research outputs found
Plasmid patterns of MDR <i>S. flexneri</i> 2a strains isolated in Dhaka, Bangladesh in between 2006 and 2011.
<p>Plasmid patterns of MDR <i>S. flexneri</i> 2a strains isolated in Dhaka, Bangladesh in between 2006 and 2011.</p
Effect of acridine orange concentration on curing efficiency of MDR <i>S. flexneri</i> 2a<sup>*</sup>.
<p>*Five hundred colonies (at each concentration) were tested for curing of the antibiotic resistant phenotype.</p
Involvement of 4.3 MDa plasmid and the sul2 gene in the resistance of sulfamethoxazole.
<p><b>a.</b> Agarose gel electrophoresis pattern of plasmid DNA isolated from SXT<sup>R </sup><i>S. flexneri</i> 2a clinical strain KH-1559 and its plasmid cured derivative. Lanes 1 and 2, represents <i>Escherichia coli</i> V517 and PDK-9 respectively as size determination markers. Lanes 3 and 4 represent KH-1559(P) SXT<sup>R</sup> strains and its 4.3 MDa plasmid cured derivative KH-1559 (C) respectively. <b>b. </b><i>sul2 and sul</i>3 gene expressions in the representative SXT<sup>R</sup> (R) and SXT<sup>S</sup> (S) strains of <i>S. flexneri</i> 2a from 2006 to 2011.</p
PFGE patterns of representative SXT<sup>R</sup> (R) and SXT<sup>S</sup> (S) <i>S. flexneri</i> 2a strains and their plasmid cured (C) derivatives.
<p>The dendrogram was constructed with Bionumerics v.4.5 software using the unweighted pair group method with arithmetic means (UPGMA). Dendrogram analysis showed that the plasmid cured strains and the parent strains are very close (95%) in their homology.</p
Phenotypic and Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Bangladesh
<div><p>Background</p><p>Resistance to cephalosporins in Enterobacteriaceae is mainly due to the production of extended-spectrum beta-lactamase (ESBL). Little is known about ESBL-producing bacteria in Bangladesh. Therefore, the study presents results of phenotypic and molecular characterization of ESBL-producing <i>Escherichia coli</i> from hospitals in Bangladesh.</p><p>Methods</p><p>A total of 339 <i>E. coli</i> isolated from patients with urinary tract and wound infections attending three different medical hospitals in urban and rural areas of Bangladesh between 2003–2007 were screened for ESBL-production by the double disk diffusion test. Isolates with ESBL-phenotype were further characterized by antibiotic susceptibility testing, PCR and sequencing of different β-lactamase and virulence genes, serotyping, and <i>XbaI</i>-macrorestriction followed by pulsed-field gel electrophoresis (PFGE).</p><p>Results</p><p>We identified 40 <i>E. coli</i> with ESBL phenotype. These isolates were resistant to ceftriaxone, ceftazidime, cefotaxime, aztreonam, cefepime, and nalidixic acid but remained susceptible to imipenem. All but one isolate were additionally resistant to ciprofloxacin, and 3 isolates were resistant to cefoxitin. ESBL genes of blaCTX-M-1-group were detected in all isolates; blaTEM-type and blaOXA-1-type genes were detected in 33 (82.5%) and 19 (47.5%) isolates, respectively. Virulence genes that are present in diarrhoeagenic <i>E. coli</i> were not found. Class-1 integron was present in 20 (50%) isolates. All the ESBL-producing <i>E</i>. <i>coli</i> isolates harbored plasmids ranging between 1.1 and 120 MDa. PFGE-typing revealed 26 different pulsotypes, but identical pulsotype showed 6 isolates of serotype O25:H4.</p><p>Conclusion</p><p>The prevalence of multidrug-resistant ESBL-producing <i>E</i>. <i>coli</i> isolates appears to be high and the majority of the isolates were positive for <i>bla</i><sub>CTX-M</sub>. Although there was genetic heterogeneity among isolates, presence of a cluster of isolates belonging to serotype O25:H4 indicates dissemination of the pandemic uropathogenic <i>E. coli</i> clone in Bangladesh.</p></div
PFGE banding patterns of <i>Xba</i>I-digested chromosomal DNA of representative ESBL-producing <i>E. coli</i> isolates.
<p>Lane 1, <i>Salmonella enterica</i> serovar Braenderup (H9812) (marker); Lanes 2-5, PFGE type A; Lane 6, PFGE type B; Lane 7, PFGE type P; Lane 8, <i>Salmonella enterica</i> serovar Braenderup (H9812) (marker); Lane 9, PFGE type E; Lane 10, PFGE type C; Lane 11, PFGE type F; Lane 12, PFGE type G; Lane 13, PFGE type B. Four isolates belonged to PFGE pattern A were isolated from UTI patients attending SMCH hospital.</p
Characterization of ESBL-producing <i>E. coli</i>.
<p>Abbreviations: UTI: Urinary Tract Infection, WI: Wound Infection. U: Urine, SWS: Surgical Wound Swab, <sup>a</sup> Sequencing performed on representative strains, (+) indicates presence and (-) indicates absence.</p><p>Characterization of ESBL-producing <i>E. coli</i>.</p
Dendrogram generated by BioNumeric software, showing distances calculated by the dice similarity index of PFGE <i>Xba</i>I profiles for <i>S. flexneri</i> 2a isolated from Bangladesh and compared with strains isolated in China.
<p>The degree of similarity (%) is shown on the scale.</p
MIC and amino acid changes in <i>gyr</i>A and <i>par</i>C in representative <i>S</i>. <i>flexneri</i> 2a strains isolated in Bangladesh and China.
<p>Abbreviations: Na, nalidixic acid; Cip, ciprofloxacin; Nor, norfloxacin; Of, ofloxacin; Azm, azithromycin; Cro, ceftriaxone, CCCP, carbonyl cyanide-m-chlorophenylhydrazone <sup>a</sup>Wild type, P1∶140, 4, 2.7, 2.1 MDa; P2∶140, 2.7, 2.1 MDa; P3∶140, 8, 2.7, 2.1MDa; P4∶140, 36–62, 2.7, 2.1MDa.</p
Transfer of resistance plasmid to <i>E. coli</i> K-12 by conjugation.
<p>Transfer of resistance plasmid to <i>E. coli</i> K-12 by conjugation.</p