Isolasi Dan Karakterisasi Lipase Dari Rebung Bambu Betung Dendrocalamusasper

This research was conducted to get the extract of rough lipase produced by bamboo shoots (Dendrocalamus asper). Lipase isolation from bamboo shoots was done by blending bamboo shoots to produce the crude extract of the enzyme. Lipase crude extracts that occurred at centrifugation at 12000 rpm for 30 minutes at 4°C. The crude extracts obtained were then tested using quantitative pH 7, temperature 30ºC and substrate 1% (v/v). Based on research there is activity of crude extract of lipase equal to 2,24 U/ml

Respon Vasodilatasi Ekstrak Metanol Daun Macaranga lamellata Whitmore Secara In Vitro

The research about in vitro vasodilatation response of methanol extract of Macaranga lamellata whitmore leaves was carried out. This research was conducted to determine the vasodilatation response of methanol extract of Macaranga lamellata Whitmore leaves on changes in aortic tone of  Wistar rat. The methods used include the in vitro methods by using separated aortic organ of  Wistar rat. Methanol extract were given cumulatively in bath organs. Positive control is used methacholine and negative control is used DMSO solvent. T-test statistical was done to determine the significant differences between groups. Phytochemical test results showed that total methanol extract contained alkaloids, polyphenol, steroids, flavonoids and saponins. Percentage change in aortic tone by using methanol extract concentration of 0.3; 1 and 3 mg/mL respectively were -7.422%; -9.641% and -10.593%. Methanol extract of Macaranga lamellata Whitmore leaves have antihypertensive potential.   Keywords: Antihypertensive, Aortic, In vitro, Macaranga lamellata Whitmore, Vasodilatatio

ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM LIPASE DARI PERKECAMBAHAN BIJI DURIAN (Durio zibethinus L)

This research was conducted to isolate and to know the optimum lipase working conditions (pH, temperature and concentration of substrate), and to know lipase activity from germination of durian seed (Durio zibethinus L.). Isolation is done by 2 stages namely, homogenization and centrifugation. Centrifugation is done at a speed of 12000 rpm with a temperature of 4°C for 30 minutes. Activity by using titration method and protein concentration using method. The optimum lipase temperature obtained was 60°C, optimum pH was 7, and the optimum substrat concentration was 1 % v/v. The lipase specific activity yielded 0,221 U/mg.   Keywords: lipase, Durio zibethinus L., Lipase specific activity

POTENSI ANTIBAKTERI EKSTRAK ETANOL MENIRAN (Phyllanthus niruri L.) TERHADAP BAKTERI Streptococcus sobrinus DAN Salmonella typhi

Meniran (Phyllanthus niruri L.) is one of the herb plants used as traditional medicine, such as toothache medicine and diarrhea medicine. The purposed of this study was to determined the type of secondary metabolites in the ethanol extract of meniran herb and to determined the minimum inhibitory concentration (MIC) value of the ethanol extract of meniran (Phyllanthus niruri L.) to Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422. The ethanol extract of meniran was tested for phytochemicals. The MIC value of the ethanol extract of meniran herb for the test bacteria was obtained by varying the concentration of the ethanol extract of meniran herbs. Phytochemical test results showed that the ethanol extract of meniran herbs contains alkaloids, phenolics and steroids. The minimum inhibitory concentration value of the ethanol extract of meniran herb at the bacteria Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 each was 0.625%. Keywords: Phyllanthus niruri L, minimum inhibitory concentration (MIC), Streptococcus sobrinus KCCM 11898, Salmonella typhi  ATCC 42

UJI FITOKIMIA DAN ANTIBAKTERI EKSTRAK ETANOL BIJI MAHONI (Swietenia mahagoni (L.) Jacq)

Phytochemical test and antibacterial activity of ethanol extract of Mahoni seed (Swietenia mahagoni (L.) Jacq) have been done. The extraction of Mahoni seed samples was carried out by the maceration method using ethanol as a solvent. An antibacterial activity test was carried out using the agar diffusion method.  Phytochemical tests results show that ethanol extract of Mahoni seeds has secondary metabolites of alkaloid, triterpenoid, and phenolic compounds. The antibacterial activity test results obtained MIC values for Streptococcus mutans bacteria were 1.25-2.5% and for Salmonella typhi bacteria at 0.625-1.25%. Ethanol extract of Mahoni seeds has broad-spectrum antibacterial activity.Uji fitokimia dan uji aktivitas antibakteri ekstrak etanol biji Mahoni (Swetenia mahagoni (L.) Jacq) telah dilakukan. Ektraksi sampel biji Mahoni dilakukan dengan metode maserasi menggunakan pelarut etanol. Uji aktivitas antibakteri dilakukan menggunakan metode difusi agar. Hasil uji fitokimia menunjukkan ekstrak etanol biji Mahoni mengandung senyawa metabolit sekunder alkaloid, triterpenoid dan fenolik. Hasil uji aktivitas antibakteri diperoleh nilai MIC untuk bakteri Streptococcus mutans   sebesar 1,25-2,5% dan untuk bakteri Salmonella typhi sebesar 0,625-1,25%. Ekstrak etanol biji Mahoni memiliki aktivitas antibakteri dengan spektrum luas

PHYTOCHEMICAL TEST AND ANTIBACTERIAL ACTIVITY OF METHANOL EXTRACT OF MENTAWA LEAF (Artocarpus anisophyllus Mig.)

Phytochemical test and antibacterial activity of methanol extract of Mentawa leaf (Articarpus anisophyllus Mig.) have been done. The extraction of Mentawa leaf samples was carried out by the maceration method using methanol as a solvent. An antibacterial activity test was carried out using the agar diffusion method. Phytochemical tests results show that methanol extract of Mentawa leaf has secondary metabolites of flavonoid, steroid, triterpenoid, phenolic, and quinone compounds. The antibacterial activity test results obtained MIC values for Salmonella typhi bacteria were 1.25-2.5%, Streptococcus mutans bacteria were 0-0,625%, Streptococcus sobrinus bacteria were 1.25-2.5% and for Propionibacterium acnes bacteria were 0.625-1.25%. Methanol extract of Mentawa leaf has broad-spectrum antibacterial activity

TEST OF AMYLASE INHIBITION ACTIVITY OF Melicope PLANTS WHICH POTENTIAL AS ANTIDIABETIC AGENTS

The test of amylase inhibition activity of the leaves of Melicope lunu-ankenda and bark of Melicope glabra (BI.) T.G. Hartley extracts were done. The objective of this study was to determine in-vitro α-amylase inhibition activity against two samples by DNS method. The result showed that two samples of Melicope lunu-ankenda leaves and bark of Melicope glabra (BI.) TG Hartley displayed the enzyme inhibitory effect with percent inhibitions of 48.71% and 46.36%. Two sample extracts can inhibit amylase. It can be suggested that about sample were potential as antidiabetic agents