Needs and readiness assessments: Tools for promoting community-university engagement with Aboriginal communities

Community-based participatory research (CBPR) is an important means of connecting the perspectives of community members with critical social issues, such as health and wellness. As beneficial as CBPR can be, effective engagement with community members remains a difficult goal to achieve. In this article, we draw on the international literature around needs and readiness assessments to explore their potential for establishing solid foundations for engaged research. We examine the stages and dimensions identified in the literature, and use these as a framework for a needs and readiness assessment project undertaken with a Métis Settlement community in Alberta, Canada. We share how the needs and readiness assessments helped to foster the emergence of community priorities, informing the next steps in research design, program content and evaluation methods, and heightening community-university engagement. It is our hope that our example of engagement, which focuses on the role of needs and readiness assessments in strengthening community-university partnerships, will better inform engagement approaches so that they become relevant, culturally appropriate and community specific. Keywords: Métis, Aboriginal, community-based participatory research, needs assessment, readiness assessment, community-university partnershi

Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo

Abstract Introduction Macrophages comprise an essential component of the mammary microenvironment necessary for normal gland development. However, there is no viable in vivo model to study their role in normal human breast function. We hypothesized that adding primary human macrophages to the murine mammary gland would enhance and provide a novel approach to examine immune-stromal cell interactions during the humanization process. Methods Primary human macrophages, in the presence or absence of ectopic estrogen stimulation, were used to humanize mouse mammary glands. Mechanisms of enhanced humanization were identified by cytokine/chemokine ELISAs, zymography, western analysis, invasion and proliferation assays; results were confirmed with immunohistological analysis. Results The combined treatment of macrophages and estrogen stimulation significantly enhanced the percentage of the total gland humanized and the engraftment/outgrowth success rate. Timecourse analysis revealed the disappearance of the human macrophages by two weeks post-injection, suggesting that the improved overall growth and invasiveness of the fibroblasts provided a larger stromal bed for epithelial cell proliferation and structure formation. Confirming their promotion of fibroblasts humanization, estrogen-stimulated macrophages significantly enhanced fibroblast proliferation and invasion in vitro, as well as significantly increased proliferating cell nuclear antigen (PCNA) positive cells in humanized glands. Cytokine/chemokine ELISAs, zymography and western analyses identified TNFα and MMP9 as potential mechanisms by which estrogen-stimulated macrophages enhanced humanization. Specific inhibitors to TNFα and MMP9 validated the effects of these molecules on fibroblast behavior in vitro, as well as by immunohistochemical analysis of humanized glands for human-specific MMP9 expression. Lastly, glands humanized with macrophages had enhanced engraftment and tumor growth compared to glands humanized with fibroblasts alone. Conclusions Herein, we demonstrate intricate immune and stromal cell paracrine interactions in a humanized in vivo model system. We confirmed our in vivo results with in vitro analyses, highlighting the value of this model to interchangeably substantiate in vitro and in vivo results. It is critical to understand the signaling networks that drive paracrine cell interactions, for tumor cells exploit these signaling mechanisms to support their growth and invasive properties. This report presents a dynamic in vivo model to study primary human immune/fibroblast/epithelial interactions and to advance our knowledge of the stromal-derived signals that promote tumorigenesis

If a tree falls, for whom does it make a sound? Multilocality and multivocality in Yukon Forests

This article explores how forests in the Yukon Territory of Canada are experienced and understood by residents in divergent ways. Through a focus onalternative interpretations and versions of forest history, I explore some of the implications of diverse forest perception for understanding multiple use issues in natural areas, as well as what can be learned about possible forest futures. As both multivocal and multilocal, many such forests have numerous individuals and groups who vie to define and articulate meanings about them. As a consequence, many meanings overlap in a single locality, the result being that a forest is not always the same place for all individuals. It also means that forest labels such as ‘used’ and ‘pristine’ are not necessarily exclusive. In the context of forest planning in the Yukon Territory, I argue that it is necessary to embrace the contested nature of place in order to understand local land values and inter-group relations