Additional file 2: Figure S1. of Seasonal variation in haematological and biochemical reference values for healthy young children in The Gambia

Median and reference intervals (2.5th-97.5th) for all haematology and biochemistry parameters over age, by gender and/or season. (PDF 1045 kb

Additional file 1: Table S1. of Seasonal variation in haematological and biochemical reference values for healthy young children in The Gambia

Proportions of observations below predicted percentiles from models with and without outliers. (DOC 64 kb

Comparison of cytokine levels between IRIS and non-IRIS patients before commencing ART. The interquartile ranges are displayed in brackets beside median values for each group and cytokine measured.

<p>Comparison of cytokine levels between IRIS and non-IRIS patients before commencing ART. The interquartile ranges are displayed in brackets beside median values for each group and cytokine measured.</p

Change in T regulatory cells in IRIS and non-IRIS patients after the initiation of antiretroviral therapy.

<p>A) Percentage of total CD3⁺CD4⁺FOXP3⁺ T cells or B) CD3⁺CD4⁺CD25⁺FOXP3⁺ after initiation of ART till six months post ART. The trend lines denote the median percent values for each group over the 6-month study period.</p

Clinical features of IRIS cases.

<p>UA = urinalysis, RLZ = right lower zone, GPC = Gram positive cocci, RUZ = right upper zone, RMZ = right middle zone, LN = lymph node, LAD = lymphadenopathy, HA = Headache.</p

Gating strategy employed to determine the percentage of CD3⁺CD4⁺FOXP3⁺ and CD3⁺CD4⁺CD25⁺FOXP3⁺ cells.

<p>A) Forward Scatter vs Side Scatter B) CD3 versus CD4 within the lymphocyte gate C) FOXP3 versus CD4 within the CD4⁺CD3⁺ gate D) FOXP3⁺ versus CD25⁺ within the CD3⁺CD4⁺ gate.</p

Classification of patients recruited to the study according to IRIS status.

<p>Classification of patients recruited to the study according to IRIS status.</p

Mean CD4% and geometric mean biomarker levels at different times before death in patients who died during follow-up compared to levels in stable subjects who were still alive at the end of follow-up.

<p>Comparisons between HIV-1 and HIV-2 patients at similar times before death are also shown. All p-values are adjusted for age of the patient.</p

Comparison of median baseline and the 5 year estimated values of CD4%, β2 m, neopterin and sUPAR for HIV-1 and HIV-2 infected subjects who were still alive after 5 years of follow up.

*<p>Median of pairwise differences (5 year estimates – baseline values).</p>**<p>Wilcoxon matched pairs signed ranks test for baseline vs 5 years.</p>***<p>Wilcoxon ranksum test comparing 5 year changes in HIV-1 patients with 5 year changes in HIV-2 patients.</p

Spearman rank correlations between the biomarkers, CD4% and viral loads (VL) for all samples in HIV-1 and HIV-2.

<p>Correlation coefficients (r_s) are shown for each comparison with the p-value and number of samples shown in brackets below.</p