Spearman’s correlation between circulation level of ANGPTL7 and TG in the non-obese and the obese adjusted for age, gender and ethnicity.

<p>Spearman’s correlation between circulation level of ANGPTL7 and TG in the non-obese and the obese adjusted for age, gender and ethnicity.</p

ANGPTL7 level after exercise.

<p><b>A</b>: Plasma level of ANGPTL7 in non-obese subjects (n = 82) before and after three months of physical exercise adjusted for age, gender and ethnicity. <b>B</b>: Plasma level of ANGPTL7 in obese subjects (n = 62) before and after three months of physical exercise adjusted for age, gender and ethnicity. <b>C</b>: Gene expression level of ANGPTL7 in adipose tissue in non-obese subjects before and after three months of physical exercise expressed as fold changes. <b>D</b>: Gene expression level of ANGPTL7 in adipose tissue in obese (n = 8) subjects before and after three months of physical exercise expressed as fold changes. * <i>P-Value</i> < 0.05 as determined using student’s t-test.</p

An Immunohistochemical staining for subcutaneous adipose biopsies from non-obese (n = 10) and obese (n = 10) non-diabetic participants using ANGPTL7 antibody.

<p><b>B</b> Immunohistochemical staining of adipose tissue from obese subjects before exercise (n = 10) and after 3 months of exercise using ANGPTL7 antibody. Arrows indicate the positive staining. Aperio software was used for quantification and the values are illustrated at the bottom as fold changes after exercise. Student <i>t-test</i> for two group analysis was done to compare ANGPTL7 expression ^between non-obese, Paired t-test between obese before and after exercise. * <i>P</i>-Value < 0.05.</p

Characteristics of the obese subjects before and after undertaking physical exercise.

<p>Characteristics of the obese subjects before and after undertaking physical exercise.</p

Characteristics of the non-obese and obese subjects included in this study.

<p>Characteristics of the non-obese and obese subjects included in this study.</p

Immunoblotting data from HEPG2 and 3T3-L1 cells showing increased ANGPTL7 protein expression and phospho-JNK after 125 μM palmitate treatment overnight compared to untreated controls.

<p>Immunoblotting data from HEPG2 and 3T3-L1 cells showing increased ANGPTL7 protein expression and phospho-JNK after 125 μM palmitate treatment overnight compared to untreated controls.</p