Effectiveness of Health Education on Knowledge and Practice Regarding the Importance of Well-Balanced Nutrition Among School Students

Introduction: Students are the future of the nation they spend more time in school then home. They need to know about the importance of a balanced diet. Balanced nutrition is important for the students, basis for lifetime health, strength, and intellectual abilities in the students. Balanced nutrition in school students give information, and skills for healthy food selection which enhance the health and prevent diseases. Methods: Quasi-experimental study was conducted to assess the effectiveness of health education on knowledge and practice regarding the importance of well-balanced nutrition among school students with (n=70) in the school of Ali Raza Abad Raiwind Road Lahore, Pakistan. The educational interventional program involves four weeks of intervention about the importance of well-balanced nutrition. Results: The results of the study showed that there is significance effectiveness of health education on knowledge and practice regarding the importance of well-balanced nutrition among school students. A total of (n=70) students participated in this study, the mean score of the pre-data collection was 17.17 with the standard deviation of 3.526, and after the educational interventional program the mean score of post data collection was 27.07 with the standard deviation 2.845, and the difference between pre and post mean score was 9.900 which was a statistically significant difference pre and post data collection. Conclusions: The study findings revealed that health education on the importance of well-balanced nutrition was effective by improving the knowledge and practice of school students. Keywords- Effectiveness. Health education. Knowledge. Practice. Well-balanced nutrition. School students. DOI: 10.7176/JHMN/69-10 Publication date: December 31st 201

Cushing syndrome in children: Pathophysiology, clinical features, diagnostic and therapeutic strategies

A rare condition called Cushing disease causes increased morbidity or mortality. Therapeutic methods such as anti-cortisol medications, bilateral adrenalectomy, or radiation procedures may therefore be required to prevent long-term dangers of hypercortisolism, such as hirsutism, moon face, facial plethora, and obesity. Endogenous hypercortisolism increases the risk of cardiovascular metabolic symptoms, osteoporosis, respiratory diseases, psychological difficulties, and infections while also having a high rate of morbidity or mortality. Significant fetal and mental complications during pregnancy are linked to Cushing syndrome. Early detection and treatment are essential. Except in the late trimester, surgery is the preferred method of treatment for Cushing syndrome during pregnancy, with medication therapy as a backup. Cushing syndrome is an endocrine illness that presents differently from other endocrine disorders, making it difficult for doctors to control

3D-Bioprinting: A stepping stone towards enhanced medical approaches

In the past few decades, tissue engineering has been seen unprecedented escalation driving the field of artificial tissue and organ construct and brought metamorphosis in regenerative medicine. Prime advancement has been attained through the expansion of novel biomanufacturing approaches to devise and convene cells in three dimensions to fabricate tissue contrive. Accompaniment manufacturing differently known as 3D bioprinting is leading prime innovation in a number of applications in life sciences such as tissue and organ construct, personalized drug dosing, cancer model and heart tissue engineering. Overall, this review summarizes most prevalent bioprinting technologies; including laser-based bioprinting, extrusion bioprinting, injection bioprinting, stereolithography as well as biomaterial such as bioink. It also explores 3D industries, approaches such as Biomimicry, autonomous self-assembly, mini tissues and biomedical applications. Existing challenges that impede clinical mileage of bioprinting are also discussed along with future prospective

A highly efficient ligation-independent cloning system for CRISPR/Cas9 based genome editing in plants

Abstract Background Most current methods for constructing guide RNAs (gRNA) for the CRISPR/Cas9 genome editing system, depend on traditional cloning using specific type IIS restriction enzymes and DNA ligation. These methods consist of multiple steps of cloning, and are time consuming, resource intensive and not flexible. These issues are particularly exacerbated when multiple guide RNAs need to be assembled in one plasmid such as for multiplexing or for the paired nickases approach. Furthermore, identification of functional gRNA clones usually requires expensive in vitro screening. Addressing these issues will greatly facilitate usage and accessibility of CRISPR/Cas9 genome editing system to resource-limited laboratories. Results To improve efficiency of cloning multiple guide RNAs for the CRISPR/Cas9 system, we developed a restriction enzyme- and ligation-independent strategy for cloning gRNAs directly in plant expression vectors in one step. Our method relies on a negative selection marker and seamless cloning for combining multiple gRNAs directly in a plant expression vector in one reaction. In addition, using the Agrobacterium-mediated transient assays, this method provides a simple in planta procedure for assaying the effectiveness of multiple gRNAs very rapidly. Conclusions For a fraction of resources used in the type IIS restriction enzyme-based cloning method and in vitro screening assays, the system reported here allows efficient construction and testing several ready-to-transfect gRNA constructs in < 3 days. In addition, this system is highly versatile and flexible, and by designing only two additional target-specific primers, multiple gRNAs can be easily assembled in any plasmid in a single reaction