Expression of GFP beyond the final extent of the retinal detachment.

<p>Dog 1 OD – this eye received an injection of AAV2(quadY-F). A. Color fundus image following retinal reattachment after the subretinal injection. The final extent of the detachment can be seen as a color change in the tapetal fundus as indicated by arrows. B. The same eye imaged to show GFP expression. C. An overlay of the fundus images in A and B. D. An enlarged view of the region in C that is within the white box. The white arrows correspond to the arrows in A. The black arrow heads point out the extent of the GFP expression which extends slightly beyond the final boundary of the retinal detachment.</p

A. Low power retinal section from eye 8-OD through the optic nerve (ON).

<p>Green shows GFP expression and blue is DAPI staining. Note that there is GFP expression in axons within the optic nerve and also within the part of the inner retina corresponding to the nerve fiber layer. The section is taken through the dorsal vasculature radiating from the optic nerve head and the blood vessels are the reason that the nerve fiber layer is not a continuous layer in this section. This region is away from the subretinal injection bleb which is why only the nerve fiber layer is expressing GFP and the outer retina is not. Inl = inner nuclear layer; onl = outer nuclear layer. Size bar 100 µm. B. Section through the optic nerve from eye 2-OD. This eye received an injection of AAV2(quadY-F). Green is GFP expression, blue DAPI staining. GFP expression is seen in the axons of the optic nerve. Size bar 250 µm.</p

Results of spread of injection bleb and extent of final GFP expression.

*<p>- indicates that bleb contacted edge of ONH following injection.</p><p>Key: NFL = Nerve fiber layer; OD = right eye; OS = left eye; ONH = optic nerve head.</p