Additional file 4: Figure S3. of Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers

Kinetics of osteogenic gene expression during osteogenic differentiation of S27 hfMSC in PCL-TCP scaffold cultures. (A) Cultures seeded with 2D monolayer-harvested (2D MNL-harv) and microcarrier-harvested (3D MC-harv) cells, (B) Cultures seeded with microcarrier-harvested (2D MNL-harv) and microcarrier-bound (3D MC-bound) cells. Two-way repeated measures ANOVA with post-hoc Tukey correction was performed between 3D MC-harv, 2D MNL-harv and 3D MC-bound cells using Graphpad, (*p < 0.05 and at least a 2-fold difference in the means). Of the multiple comparisons performed, data representing a single comparison is shown: (A) 3D MC-harv vs 2D MNL-harv, (B) 3D MC-bound vs 2D MNL-harv. (TIFF 544 kb

Additional file 5: Table S1. of Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers

List of Taqman Gene Expression Assay IDs for genes investigated by qPCR in this study. (DOCX 13 kb