3 research outputs found
Detection of Nicotine Effect on Colon Cells in a Plasmonic Platform
Introduction: Smoking as one of the causes of various diseases has encouraged worldwide studies on its adverse pharmacological effects on different organs. Nicotine may influence the smooth muscles of the colon and subsequently the gut motility, which leads to a change in the moving rate of digested material through the gastrointestinal tract.Methods: Among various techniques, optical detection methods benefit from non-contact and high-sensitivity for studying the early effect of nicotine on the cells. Thus, we used an optically ellipsometric method to get the fast and sensitive nicotine effect on the colon cell. Two-dimensional plasmonic platforms by gold deposition onto the polydimethylsiloxane polymer (PDMS) patterned substrate were used as the guest medium of the cell and the sample was excited by all of the visible region wavelengths at different exposure time and maintenance time.Results: Our results showed that the phase difference between each polarization increased by augmenting the exposure time of smoke over the cell at a fixed maintenance time and there was a general red-shift by increasing the maintenance time at a fixed exposure time.Conclusion: Using different exposure time to cigarette smoke, we optically showed that the cigarette containing the addicting chemical of nicotine had a direct effect on the cultured colon cells on our 2D biocompatible plasmonic chip. It demonstrated considerable changes in the amplitude and phase of the interacted light by injecting nicotine into the system with the aid of the label-free and non-invasive plasmonic technique
Optical Fiber Biosensor toward E-coli Bacterial Detection on the Pollutant Water
In this study, Zinc oxide (ZnO) nanorods based fiber optic biosensor has been reported for rapid and sensitive detection of Escherichia Coli (E-coli). A thin layer of Gold nanoparticles (Au) (around 50 nm) is coated on the tip of a multimode plastic optical fiber. ZnO Nanorods are grown on Au layer thorough hydrothermal technique. This sensor showed a very fast response within the first 10 second of contacting the present of polluted water with E-coli Different concentrations of E. coli from (1000 to 4000 CFU/ml) have been tested and a sharp trend of sensitivity was observed. This sensing platform shows promising potential for regular water and food quality monitoring of various pathogenic microorganisms
Effect of Methadone and Tramadol Opioids on Stem Cells Based on Integrated Plasmonic-Ellipsometry Technique: Effect of Opioids on Stem Cells Based on plasmonics
Introduction: Plasmonic biosensors provide high sensitivity in detecting the low amount of biomarkers and pharmaceutical drugs. We studied the mesenchyme cell activity under the treatment of common sedative drugs of methadone and tramadol using the integrated plasmonic-ellipsometry technique.Methods: Mesenchymal stem cells were cultured on patterned plasmonic chips under the treatment of methadone and tramadol drugs. Three cultured chips were kept non-treated as the control ones. The plasmonic-ellipsometry technique was applied to study the signaling characteristic of the cells affected by these two drugs. In this technique, optical information regarding the amplitude ratio and phase change between p- and s-polarized light was recorded.Results: This drug treatment could affect the spectral plasmonic resonance and subsequently the phase shift (Δ) and the amplitude ratio (Ψ) values under p- and s-polarized impinging light. A more significant Δ value for tramadol treatment meant that the phase split was larger between p- and s-polarized light. Tramadol also had more prominent absolute Δeff and Ψeff values in comparison with methadone.Conclusion: We showed that tramadol caused more contrast in phase shift (Δ) and amplitude ratio (Ψ) between p- and s-polarized impinging light for cultured stem cells in comparison with methadone. It means that tramadol differentiated more the optical responses for p- and s-polarized lights compared to methadone. Our proposed technique possesses the potential of quantitative and qualitative analysis of drugs on humans even on a cell scale.
DOI:10.34172/jlms.2021.4