Effect of gibberellic acid on growth and flowering attributes of African marigold (Tagetes erecta) in inner terai of Nepal

A field experiment was conducted at Bangaun, Lamahi-3, Dang, Nepal to study the effect of GA3 on growth and flowering attributes of African marigold (Tagetes erecta) in Inner Terai of Nepal. The experiment consists of three replications and 8 treatments and laid out in a randomized complete block design- consisting of various concentrations of GA3 viz. 0ppm, 50ppm, 100ppm, 150ppm, 200ppm, 250ppm, 300ppm, and 350ppm. Kolkata local variety of African marigold was tested. The study revealed that among different concentrations of GA3, 300ppm showed the tallest plant height (72.93cm) and the highest basal diameter (1.49cm). Maximum numbers of primary branches (3.11) and the greatest plant spread (32.11cm) were obtained from 250ppm; similarly, maximum numbers of secondary branches (13.80) were recorded in 350ppm. In the case of floral parameters both 100ppm and 350ppm recorded earlier days to 50% flowering (44.00 days each), days for 100% flowering was recorded almost similar in every treatment that sticks around 54 and 55 days, maximum diameter (5.370cm) of flowers were obtained from 50ppm, the greatest fresh weight (6.180g) was recorded in 350ppm, 250ppm showed a maximum number of flower per plant (104.13), similarly, a longer duration of flowering (58 days) was recorded in 300ppm. Among all treatments, the 250ppm level of GA3 was found to be most suitable in terms of production perspective

Analysis of Methanol Content in Different Varieties of Traditionally Fermented Alcohol Found in Chandannath Municipality of Jumla District, Nepal

Background: Alcohol poisoning associated with traditionally fermented alcoholic beverages have been reported in Nepal with the constituents of these beverages not being quantified usually. In traditionally fermented alcoholic beverages, methanol, due to its cheaper cost and similar physiochemical properties with ethanol, is adulterated most commonly and readily. The objective of the present study was to quantify four different varieties of traditionally fermented alcoholic beverages available in Chandannath, Jumla for methanol level. Methods: The present study is a cross-sectional study in which four different types of traditionally fermented alcoholic beverages were collected from Chandannath, Jumla and they were analyzed for methanol content. From Chandannath municipality, the ward number two was selected for collection of samples. Further, from this ward, four different households regularly involved in brewing local alcohol were also randomly selected. These households belonged to three different ethnic groups (Indo-Aryan, Tibeto-Burman and Newar) and these samples were chhyang (sample- 1), local raksi (sample- 2), nigar (sample- 3) and local apple cider (sample- 4). In air tight containers, the samples were sealed and transported to Zest laboratory, Bhaktapur, Nepal for quantification of methanol using Gas Chromatography. Results: The residents in half of these four households belonged to the Tibeto-Burman ethnic groups. Methanol was detected in two samples among the four analyzed beverages. These samples, sample- 2 and sample- 4, on quantification showed the concentrations of 10.050ppm and 13.721ppm of methanol respectively. Conclusion: Among four samples of locally brewed alcoholic beverages, methanol was detected in two samples, but at a concentration below the level that would be considered toxic. This finding emphasizes the importance of conducting larger-scale quantification of traditionally fermented alcohol to mitigate the various health risks associated with its potential toxicity, as these beverages are currently not being quantified

The Immunoreactivity of Calbindin on Cerebellum Cortex and Hippocampus of Mice Brain After One Week Exposure To Radiofrequency Radiation.

Background &amp; Objectives:Radiofrequency radiation affects the calcium permeability and accordingly this induces pathophysiological changes in the brain and its subsequent output in the brain as tumor genesis, neural degeneration, and cognition or behavior changes. Hence, we investigated the effects of radiofrequency (RF) radiation on calcium binding proteins, reactive gliosis, and tumor genesis in mouse brain. Materials &amp; Methods:Mice were exposed to radiofrequency radiation in the cage chamber with average specific absorption rate (SAR) of 1.6 W/kg and 4.0 W/Kg for one hour per day for five days. For histochemical studies, radiofrequency exposed brains were compared with sham control using calbindin D-28k antibody.Results:The immunoreactivity of calbindin D-28k, a marker for calcium homeostasis was found to increase in dose-response manner in hippocampus and cerebellar cortex.Conclusion:The short-term exposure to 835 MHz RF could induce disruption of calcium homeostasis and resulting neurobehavioral changes or brain tumor. </p

The impact of climate change on insect pest biology and ecology: Implications for pest management strategies, crop production, and food security

The explosive expansion of the global population and technological progress has greatly influenced agriculture and food production. However, this progress is threatened by climate change, which unleashes a slew of issues like carbon dioxide (CO2) increases, frequent droughts, and temperature shifts that present a substantial obstacle to crop yields and food security. The ramifications of these climatic factors on insect pest biology and ecology are profound, given that these pests depend heavily on these factors. Since crop productivity is tightly connected to both insect pests and these climate variables, changes in these factors can significantly impact crop yields. Therefore, it is imperative to comprehend the impact of climate change on insect pests to manage them effectively and ensure sufficient food production. This review examines the climate change effect on insect pest biology and ecology and explores the potential use of modern pest monitoring technologies and prediction tools to devise effective pest management strategies to improve crop production and food security

A comparative study of the pharmacokinetics of traditional and automated dosing/blood sampling systems using gabapentin

Objective: The present study was undertaken to investigate the pharmacokinetics (PKs) of gabapentin as determined by traditional manual blood sampling and by using an automated dosing/blood sampling technique in awake and freely moving rats using combined liquid chromatography tandem mass-spectrometry (LC-MS/MS). Materials and Methods: PK comparisons were conducted by allocating rats into two groups; an automated dosing/blood sampling (ADI/ABS) group (IV study, n = 6 and intragastric study, n = 6) and a manual group (IV study, n = 6 and oral study, n = 6). A series of blood samples from carotid artery were taken at specified times and analyzed using a validated LC-MS/MS method. Various PK parameters like area under curve (AUCinf), maximum concentration, time to reach maximum concentration, terminal half life, distribution volume at the steady state, and total clearance were calculated and the two study groups were compared with respect to these parameters. Results: Significant differences in PK parameters were observed between the manual group and the ADI/ABS group and respective bioavailability were measured (46.82 ± 19.45% and 61.54 ± 21.23%, respectively) which is 1.31-fold difference (P = 0.0051, P<0.05). Conclusion: The described ADI/ABS method was found to be a useful drug development tool for accelerating the pace of preclinical in vivo studies and for obtaining reliable and accurate PK parameters even from single animals as it minimized interanimal and physiological variations

Effect of Gibberellic Acid on Growth and Flowering Attributes of African Marigold (Tagetes Erecta) in Inner Terai of Nepal

A field experiment was conducted at Bangaun, Lamahi-3, Dang, Nepal to study the effect of GA3 on growth and flowering attributes of African marigold (Tagetes erecta) in Inner Terai of Nepal. The experiment consists of three replications and 8 treatments and laid out in a randomized complete block design- consisting of various concentrations of GA3 viz. 0ppm, 50ppm, 100ppm, 150ppm, 200ppm, 250ppm, 300ppm, and 350ppm. Kolkata local variety of African marigold was tested. The study revealed that among different concentrations of GA3, 300ppm showed the tallest plant height (72.93cm) and the highest basal diameter (1.49cm). Maximum numbers of primary branches (3.11) and the greatest plant spread (32.11cm) were obtained from 250ppm; similarly, maximum numbers of secondary branches (13.80) were recorded in 350ppm. In the case of floral parameters both 100ppm and 350ppm recorded earlier days to 50% flowering (44.00 days each), days for 100% flowering was recorded almost similar in every treatment that sticks around 54 and 55 days, maximum diameter (5.370cm) of flowers were obtained from 50ppm, the greatest fresh weight (6.180g) was recorded in 350ppm, 250ppm showed a maximum number of flower per plant (104.13), similarly, a longer duration of flowering (58 days) was recorded in 300ppm. Among all treatments, the 250ppm level of GA3 was found to be most suitable in terms of production perspective

Chronic 835-MHz radiofrequency exposure to mice hippocampus alters the distribution of calbindin and GFAP immunoreactivity

Exponential interindividual handling in wireless communication system has raised possible doubts in the biological aspects of radiofrequency (RF) exposure on human brain owing to its close proximity to the mobile phone. In the nervous system, calcium (Ca ) plays a critical role in releasing neurotransmitters, generating action potential and membrane integrity. Alterations in intracellular Ca concentration trigger aberrant synaptic action or cause neuronal apoptosis, which may exert an influence on the cellular pathology for learning and memory in the hippocampus. Calcium binding proteins like calbindin D28-K (CB) is responsible for the maintaining and controlling Ca homeostasis. Therefore, in the present study, we investigated the effect of RF exposure on rat hippocampus at 835 MHz with low energy (specific absorption rate: SAR = 1.6 W/kg) for 3 months by using both CB and glial fibrillary acidic protein (GFAP) specific antibodies by immunohistochemical method. Decrease in CB immunoreactivity (IR) was noted in exposed (E1.6) group with loss of interneurons and pyramidal cells in CA1 area and loss of granule cells. Also, an overall increase in GFAP IR was observed in the hippocampus of E1.6. By TUNEL assay, apoptotic cells were detected in the CA1, CA3 areas and dentate gyrus of hippocampus, which reflects that chronic RF exposure may affect the cell viability. In addition, the increase of GFAP IR due to RF exposure could be well suited with the feature of reactive astrocytosis, which is an abnormal increase in the number of astrocytes due to the loss of nearby neurons. Chronic RF exposure to the rat brain suggested that the decrease of CB IR accompanying apoptosis and increase of GFAP IR might be morphological parameters in the hippocampus damages

Effect of 835\ua0MHz radiofrequency radiation exposure on calcium binding proteins in the hippocampus of the mouse brain

Worldwide expansion of mobile phones and electromagnetic field (EMF) exposure has raised question of their possible biological effects on the brain and nervous system. Radiofrequency (RF) radiation might alter intracellular signaling pathways through changes in calcium (Ca ) permeability across cell membranes. Changes in the expression of calcium binding proteins (CaBP) like calbindin D28-k (CB) and calretinin (CR) could indicate impaired Ca homeostasis due to EMF exposure. CB and CR expression were measured with immunohistochemistry in the hippocampus of mice after EMF exposure at 835\ua0MHz for different exposure times and absorption rates, 1\ua0h/day for 5\ua0days at a specific absorption rate (SAR) = 1.6\ua0W/kg, 1\ua0h/day for 5\ua0days at SAR = 4.0\ua0W/kg, 5\ua0h/day for 1\ua0day at SAR = 1.6\ua0W/kg, 5\ua0h/day for 1\ua0day at SAR = 4.0\ua0W/kg, daily exposure for 1\ua0month at SAR = 1.6\ua0W/kg. Body weights did not change significantly. CB immunoreactivity (IR) displayed moderate staining of cells in the cornu ammonis (CA) areas and prominently stained granule cells. CR IR revealed prominently stained pyramidal cells with dendrites running perpendicularly in the CA area. Exposure for 1\ua0month produced almost complete loss of pyramidal cells in the CA1 area. CaBP differences could cause changes in cellular Ca levels, which could have deleterious effect on normal hippocampal functions concerned with neuronal connectivity and integration