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    Additional file 4: Figure S2. of Marked differences in tight junction composition and macromolecular permeability among different intestinal cell types

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    Top row: Immunofluorescence image of MUC2 showing the same organoid with different focus levels to show that goblet cells may appear filled with mucus or appear empty depending on the focus level (arrows). Bottom row: Images depicting organoid paraffinized sections incubated with the secondary antibody only, to show potential autofluorescence in the green (488) or red (546) spectrum. (PDF 755 kb
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