Escherichia coli as uropathogen: antibiotic susceptibility profiling

Background: Empirically chosen antibiotics based on the local resistance pattern of uropathogens remain the principle treatment of urinary tract infections (UTI).Methods: Antibiogram of most frequent uropathogen was determined. Based on the antibiogram result, authors compared effectiveness of drugs recommended for UTI by National centre for disease control (NCDC), India, and assessed age and gender based variability in the effectiveness of these drugs.Results: 1278 urine samples were accounted, of which 405 samples showed significant growth. E. coli was the most common uropathogen (n=146, 36%) followed by enterococcus species (31%) and Klebsiella pneumoniae (10%). Using McNemar’s test authors found that nitrofurantoin (90% sensitivity) was statistically the most effective drug among drugs recommended by NCDC for uncomplicated cystitis. Furthermore, authors used Fisher’s exact test on adults and paediatrics and found that significant difference in effectiveness was observed for nitrofurantoin (p-value <0.001) and cotrimoxazole (p-value 0.034). Using logistic regression, authors found that with age, effectiveness of ciprofloxacin and cotrimoxazole deteriorate significantly (p-value 0.021 and 0.002 respectively). Additionally, authors observed that cotrimoxazole has significantly better efficacy in males compared to females (p-value 0.022).Conclusions: In accordance with present study, nitrofurantoin can be used as first line treatment for uncomplicated cystitis. Age and gender should be considered while prescribing empirical treatment for UTI. Periodic surveillance should be carried out to identify the on-going pattern of antibiogram to update the guideline for empirical therapy

Genotypic and Serotypic confirmations of Bacterial community to Kotumsar cave for occupational safety of cave workers and visitors from pathogenic threats

Background: Bacterial communities exist everywhere in the universe so in the caves. Kotumsar cave is the one of the renowned tourist place in India therefore high risk of anthropogenic pressure in this     ecological niche. Objective: In the present study some pathogenic and nonpathogenic soil bacterial communities earlier isolated and characterized from different microhabitats of Kotumsar cave have been further confirmed by using serological and molecular technique i.e. applying Amplified Ribosomal DNA Restriction Analysis (ARDRA). Methods: Identified bacterial cultures were re-cultured for 16SrDNA   analysis. Cluster analysis was used to analyze the restriction pattern generated by the enzyme by the using NTSYS-pc and UPGMA software and as well serotyping is done by Prescott’s method. Result: Total twelve bacteria were selected for serological and molecular characterization. There were two most suitable enzymes EcoRI and AluI which shows typical molecular characteristics between the similar  genuses. Conclusion: This piece of work confirms some pathogenic bacteria in Kotumsar cave’s     habitat. Though they are not highly pathogenic but repeated visiting in this cave environment may cause infectivity accordingly proper safety precaution must followed after visiting the cave.

Escherichia coli as uropathogen: antibiotic susceptibility profiling

Background: Empirically chosen antibiotics based on the local resistance pattern of uropathogens remain the principle treatment of urinary tract infections (UTI).Methods: Antibiogram of most frequent uropathogen was determined. Based on the antibiogram result, authors compared effectiveness of drugs recommended for UTI by National centre for disease control (NCDC), India, and assessed age and gender based variability in the effectiveness of these drugs.Results: 1278 urine samples were accounted, of which 405 samples showed significant growth. E. coli was the most common uropathogen (n=146, 36%) followed by enterococcus species (31%) and Klebsiella pneumoniae (10%). Using McNemar’s test authors found that nitrofurantoin (90% sensitivity) was statistically the most effective drug among drugs recommended by NCDC for uncomplicated cystitis. Furthermore, authors used Fisher’s exact test on adults and paediatrics and found that significant difference in effectiveness was observed for nitrofurantoin (p-value &lt;0.001) and cotrimoxazole (p-value 0.034). Using logistic regression, authors found that with age, effectiveness of ciprofloxacin and cotrimoxazole deteriorate significantly (p-value 0.021 and 0.002 respectively). Additionally, authors observed that cotrimoxazole has significantly better efficacy in males compared to females (p-value 0.022).Conclusions: In accordance with present study, nitrofurantoin can be used as first line treatment for uncomplicated cystitis. Age and gender should be considered while prescribing empirical treatment for UTI. Periodic surveillance should be carried out to identify the on-going pattern of antibiogram to update the guideline for empirical therapy