3 research outputs found
Antimicrobial Silver Nanoclusters Bearing Biocompatible Phosphorylcholine-Based Zwitterionic Protection
Infection
is one of the most serious issues in medical treatments
leading to the development of several antimicrobial agents. In particular,
silver ions released from silver substrates is well-known as a reliable
antimicrobial agent that either kills the microorganisms or inhibits
their growth. Unfortunately, many reports have shown that silver-based
antimicrobial agents are toxic for human cells as well. To improve
the biocompatibility of silver antimicrobial agents, we have synthesized
thiol-terminated phosphorylcholine (PC-SH)-protected silver nanoclusters
(PC–AgNCs) via strong thiol–metal coordination with
controlled ultrasmall size of the clusters. A change in plasmon-like
optical absorption was studied to affirm the successful synthesis
of small thiolated AgNCs through the absorption spectra that become
molecular-like for the AgNCs. We observed that PC–AgNCs were
spherical with an average diameter of <2 nm. The ultrasmall size
clusters were exceedingly immobilized by the PC-SH on the surface,
resulting in excellent biocompatibility and antibacterial activity
simultaneously. The biocompatible, antimicrobial PC–AgNCs exhibit
interesting advantages compared with other silver antimicrobial agents
for medical applications
Patterned Poly(acrylic acid) Brushes Containing Gold Nanoparticles for Peptide Detection by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry
Patterned polyÂ(acrylic acid) (PAA)
brushes was successfully generated
via photolithography and surface-initiated reversible addition–fragmentation
chain transfer (RAFT) polymerization of acrylic acid as verified by
water contact angle measurements and FT-IR analysis. The carboxyl
groups of PAA brushes can act as reducing moieties for in situ synthesis
of gold nanoparticles (AuNPs), without the use of additional reducing
agent. The formation of AuNPs was confirmed by transmission electron
microscopy and X-ray photoelectron spectroscopy. The glass surface-modified
by PAA brushes and immobilized with AuNPs (AuNPs-PAA) can be used
as a substrate for SALDI-MS analysis, which is capable of detecting
both small peptides having <i>m</i>/<i>z</i> ≤
600 (glutathione) and large peptides having <i>m</i>/<i>z</i> ≥ 1000 (bradykinin, ICNKÂQDCPÂILE) without
the interference from matrix signal suggesting that AuNPs were stably
trapped within the PAA brushes and the carboxyl groups of PAA can
serve as internal proton source. By employing AuNPs as the capture
probe, the AuNPs-PAA substrate can selectively identify thiol-containing
peptides from the peptide mixtures with LOD as low as 0.1 and 0.05
nM for glutathione and ICNKÂQDCPÂILE, respectively. An ability
to selectively detect ICNKÂQDCPÂILE in a diluted human serum
is also demonstrated. The patterned format together with its high
sensitivity and selectivity render this newly developed substrate
a potential platform for high-throughput analysis of other biomarkers,
especially those with low molecular weight in complex biological samples
Optical Properties of 2‑Methacryloyloxyethyl Phosphorylcholine-Protected Au<sub>4</sub> Nanoclusters and Their Fluorescence Sensing of C‑Reactive Protein
We present the solution synthesis
of thiolated 2-methacryloyloxyethyl
phosphorylcholine (MPC)-protected Au nanoclusters (NCs). This water-soluble
lipid-mimetic MPC was first used for the size focusing synthesis of
thiolate (SR)-protected Au<sub><i>n</i></sub>(SR)<sub><i>m</i></sub> NCs. Au<sub>25</sub>(MPC)<sub>18</sub> and Au<sub>4</sub>(MPC)<sub>4</sub> NCs are selectively synthesized, without
the need for electrophoretic or chromatographic isolation of size
mixed products, by including ethanol or not in the solvent. The Au<sub>4</sub>(MPC)<sub>4</sub> NCs emit at yellow wavelengths (580–600
nm) with a quantum yield (3.6%) and an average lifetime of 1.5 ÎĽs.
Also for the first time, we report C-reactive protein (CRP) sensing
using Au NCs, with a detection limit (5 nM) low enough for the clinical
diagnosis of inflammation. This is based on the quenching effect of
specific CRP–MPC interactions on the fluorescence of the Au<sub>4</sub>(MPC)<sub>4</sub> NCs