1 research outputs found
Development of recombinant sialidase (NanH) protein-based Indirect-ELISA for epidemiological survey of anti-Pasteurella multocida antibodies in bovines
697-704Haemorrhagic septicaemia (HS) is a highly contagious and fatal disease of cattle and buffaloes and causes major
economic losses to farmers. Though indirect hemagglutination (IHA) test has been used to detect a specific antibody against
P. multocida, it has low specificity for sero-diagnosis of HS. Therefore, development of a rapid, highly sensitive and
specific serological test is a prerequisite for detection of antibodies against HS. In this context, we explored an in-house
ELISA method using recombinant antigens for detection of antibodies against P. multocida in bovines. nanH gene from
P. multocida B:2 strain P52 was cloned and the recombinant mature protein with a C- and N-terminal truncation was
produced as a fusion protein (∼63 kDa) in Escherichia coli. The immunogenic potential of purified rNanH-Tr was assessed
by the Western blot method using specific anti-rNanH-Tr antibody responses in sera collected from immunized rabbits. An
indirect-ELISA based on rNanH-Tr was developed and optimized. Furthermore, the rNanH-Tr ELISA was applied to screen
bovine serum samples (n=250). The receiver operating characteristic curve analysis for the detection of anti P. multocida
specific antibodies indicated a diagnostic sensitivity of 86.2 (CI 73.26-96.80%) and specificity of 80.0 (63.06- 91.56%). No
cross reactivity was noted with antibodies against other bovine diseases (e.g., foot-and-mouth disease and brucellosis).
Screening of random bovine serum samples showed a 22% sero-positivity for anti P. multocida specific antibodies