Spontaneous Rotation of a Toric Implantable Collamer Lens

We present a case of toric implantable collamer lens (TICL) spontaneous rotation in a patient with myopic astigmatism. A 23-year-old female underwent TICL implantation. Preoperative uncorrected visual acuity (UCVA) was 20/800 and 20/1200, respectively, with −7.75 −4.25 × 0° and −8.25 −5.25 × 180°. The left eye achieved an UCVA of 20/30. After 3 months of successful implantation of TICL in the left eye, the patient presented with a sudden decrease in visual acuity in the left eye. UCVA was 20/100 with a refraction of +2.50 −4.50 × 165°. We observed the toric marks with a 30° rotation from the original position and decided to reposition the TICL, obtaining a final UCVA of 20/25, which remained stable at 6 months' follow-up. TICL can present a considerable rotation that compromises visual acuity. The relocation of TICL is a safe and effective procedure to recover visual acuity due to significant spontaneous TICL rotation

Gasificación de carbones colombianos en lecho fluidizado

IP 1118-06-192-95gasificacion de carbon / Alonso Ocampo O., Jaime Aguirre C. yCarlos Londoño G. -- En: Carbon : factor de;desarrollo integral siglo XXI. Memorias / Congreso Nacional yInternacional de Ciencia y Tecnologia del;Carbon. (4 y 2 : 1998 mayo 19-22 : Paipa (Boyaca) Colombia. --[s.l. : s.n.], 1998. -- p. -- Balance de masa y;energia de un gasificador basado en equilibrio quimico / E. Arenas ... [etal.]. -- En: Carbon : factor de;desarrollo integral siglo XXI. Memorias / Congreso Nacional yInternacional de Ciencia y Tecnologia del;Carbon. (4 y 2 : 1998 mayo 19-22 : Paipa (Boyaca) Colombia. --[s.l. : s.n.], 1998. -- p. -- Effect of;pyrolysis conditions on the CO2 catalytic gasification ofchar/A. Molina... [et al.]. -- En: Proceedings /;International Conference on Coal Science (9 : 1997 Sept. 7-12:Essen, Germany). -- Essen : [s.n.], 1997. --;p. -- ARTICULO(S) EN REVISTA: Reactivity of coal gasificationwith steam and CO2 / Alejandro Molina and Fanor;Mondragon. -- En: Fuel. -- Vol. 77, no. 15 (1998); p. 1831-1839.-- ISSN 00162361 -- CO2 strong chemisorption;as an estimate of coal char gasification reactivity / AlejandroMolina, Alejandro Montoya, Fanor Mondragon.; En: Fuel. -- Vol. 78 (1999); p. 971-977. -- ISSN 001623-61-Prediccionde los principales productos de la;gasificacion del carbon / Erika Arenas Castiblanco ... [etal.].'-- en: energia y computacion. -- Vol. 8, no.;1 (ene.-jun. 1999); p. 62-66. -- ISSN 01215299.;and Alejandro Molina. -- En: Prospects for coal science in21stcentury ed. Pekin (Beijing) : Shanxi Science &;Technology Press, 1999. -- p. -- PONENCIA(S) EN CONGRESO:Actividad catalitica de hierro y niquel en la;gasificacion con CO2 de semicoques de Amaga y Titiribi / A. Montoya ... [et al.]. -- En: Carbon : factor de;desarrollo integral siglo XXI. Memorias / Congreso Nacional yInternacional de Ciencia y Tecnologia del;Carbon. (4 y 2 : 1998 mayo 19-22 : Paipa (Boyaca) Colombia. --[s.l. : s.n.], 1998. -- p. -- Efecto de las;condiciones de pirolisis en la gasificacion de CO2 de semicoquescon K como catalizador / A. Molina, F.;Mondragon. -- En: Carbon : factor de desarrollo integral sigloXXI. Memorias / Congreso Nacional y;Internacional de Ciencia y Tecnologia del Carbon. (4 y 2 :1998mayo 19-22: Paipa (Boyaca) Colombia. -- [s.l.;: s.n.], 1998. -- p. -- Utilizacion de la quimisorcion deCO2,en el estudio de la reactividad de semicoques;durante la gasificacion con CO2 / A. Molina, F. Mondragon.--En: Carbon :factor de desarrollo integral siglo;XXI. Memorias / Congreso Nacional y Internacional de Ciencia yTecnologiadel Carbon. (4 y 2 : 1998 mayo 19-22;: Paipa (Boyaca) Colombia. -- [s.l. : s.n.], 1998. -- p. -'- Simulacion y diseño de una planta piloto de;CAPITULO(S) EN LIBRO: Evolution of pore structure and surfacearea duringgasification of coal char with CO2;and H2O / Alejandro Montoya, Elizabeth Florez and Fanor Mondragon. -- En:Prospects for coal science in 21st;century ed. Pekin (Beijing) : Shanxi Science & TechnologyPress,1999. --p. -- CO2 chemisorption on chars;loaded with K, Fe and Ni and its relation to CO2 gasificationreactivity /Fanor Mondragon, Alejandro Montoy

Follow-up of <i>T. cruzi</i> infected patients using qPCR.

<p>A. Follow-up of orally infected cases from Chacao, Caracas, Venezuela. Years pos-treatment (ys pos-T) are represented in the <i>x</i>-axis. Parasite equivalents (par. eq.) were estimated using a Silvio X-10 (TcI) calibration curve. Case 1- Pre-T: 5.23 log₁₀ par. eq./10 mL; 2 ys pos-T: 1.88 log₁₀ par. eq./10 mL. Case 2- Pre-T: 3.78 log₁₀ par. eq./10 mL; 2 ys pos-T: 1.83 log₁₀ par. eq./10 mL. Case 3- Pre-T: 2.94 log₁₀ par. eq./10 mL; 2 ys pos-T: 1.88 log₁₀ par. eq./10 mL. B. A 42 year-old seronegative man received kidney transplantation from a seropositive cadaveric donor. Progression of parasitic load after transplantation is shown as well as post-treatment follow-up. The quantification was estimated using a Cl-Brener (TcVI) calibration curve. Days pos-Transplantation (Tx) are represented in the <i>x</i>-axis. The number of par. eq./10 mL of blood is represented in the <i>y-</i>axis, in a log-scale. Arrow marks initiation of Benznidazole treatment. ND: not detectable. The line indicates LOQ (1.185 log₁₀ par. eq./10 mL) derived from analysis of CL-Brener (TcVI) spiked samples. Discontinued line: parasitic loads in Chacao patients were estimated with Silvio X-10 (TcI) calibration curves.</p

Parasitic loads in Chagas disease clinical groups.

<p>CD: Chagas disease; Cong: Congenital; Pos: Positivity; Per: Percentile; par. eq./10 mL: parasite equivalents in 10 mL of blood.</p>a<p>Three out of 27 newborns to seropositive mothers were diagnosed as congenitally infected by means of conventional diagnosis.</p

Estimation of Precision of the qPCR assay.

<p>S_r: estimate of repeatability standard deviation (within-run precision); B: standard deviation of the daily means; N: number of replicate analyses per run; S_t: estimate of within-device or within-laboratory precision standard deviations (S_t = [B²+(N−1)/N*S_r²]^1/2); CV: coefficient of variation; log₁₀ par. eq./10 mL: logarithmic values of parasite equivalents in 10 mL of blood.</p

Exclusivity assay with other Trypanosomatids.

<p>Results are shown as mean Ct obtained from duplicates of each DNA concentration.</p>a<p>Only one replicate was detected. Ct: threshold cycle; Undet.: not detectable.</p

Estimation of the Limit of quantification of qPCR method.

<p>The LOQ was derived from a 20% threshold value for the coefficient of variation (CV) of measurements obtained in the precision experiments reported in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002000#pntd-0002000-t004" target="_blank">Table 4</a>. Linear least squares curve fit for relationship between CV and parasite concentration (log₁₀ par. eq./10 mL) using SigmaPlot 10.0 for Windows (SPSS, Chicago, IL). The derivation of LOQ_20%CV is illustrated by dotted lines.</p

Anticipated reportable range and linearity of qPCR method.

<p>Multiplex TaqMan qPCR strategy was carried out with spiked GEB samples containing parasite stocks belonging to TcI and TcVI in ten concentrations spanning 10⁶ to 0.625 par. eq./10 mL, tested in triplicate. Assigned values were plotted on the <i>x</i> axis versus measured values (converted to log₁₀) on the <i>y</i> axis using SigmaPlot 10.0 for Windows (SPSS, Chicago, IL). Linear regression analysis rendered the equation y = 1.013x+0.058 (R² = 0.992) for TcI, and y = 1.001x+0.005 (R² = 0.998) for TcVI.</p

Estimation of IAC amplification in blood specimens from different clinical groups.

a<p>Sample PCC 331: qPCR positive, 0.69 log₁₀ par. eq./mL, IAC Ct 19.20.</p><p>Nb: Newborn; UCB, umbilical cord blood; PB: peripheral blood.</p