Demographic and obstetric characteristics of the study population.

<p>HW. Healthy women.</p><p>HP. Healthy pregnant women.</p><p>ILI. Pregnant women with influenza-like illness.</p><p>PH1N1. H1N1pdm2009 virus-infected pregnant women.</p><p>Gw. Gestational weeks.</p><p>n/a not applicable.</p><p>*Fisher’s exact test.</p><p>Demographic and obstetric characteristics of the study population.</p

Serum cytokine concentrations in HW, HP, ILI and PH1N1 women.

<p>Pro-inflammatory TNF-α (a), IL-1β (b) and IL-6 (c) and anti-inflammatory IL-10 (d) cytokines were quantified using a CBA system with flow cytometry. The Kruskal-Wallis test with Dunn’s multiple comparison post-test was performed using the GraphPad Software. The significance values were *p<0.05, **p<0.01, ***p<0.001.</p

Clinical manifestations in the ILI and PH1N1 women.

<p>ILI: Pregnant women with influenza-like illness.</p><p>PH1N1: Confirmed H1N1pdm2009 virus-infected pregnant women.</p><p>*Fisher’s exact test.</p><p>Clinical manifestations in the ILI and PH1N1 women.</p

Higher percentage of CD69+ lymphocytes in H1N1pdm2009 virus-infected pregnant women.

<p>The peripheral blood leukocytes from the HW, HP, ILI and PH1N1 women were immunostained with CD3-, CD19-, CD14- and CD69-specific antibodies and analyzed by flow cytometry. Representative data of each group are shown in the dot plots for CD69+CD3+ cells (a). The distribution of CD69 expression on CD3− (b), CD19− (c) or CD14− (d) gated cells. The Kruskal-Wallis test with Dunn’s multiple comparison post-test was performed using the GraphPad Software. The significance values were *p<0.05, **p<0.01, ***p<0.001.</p

Distribution of leukocytes in the blood.

<p>HW. Healthy women.</p><p>HP. Healthy pregnant women.</p><p>ILI. Pregnant women with influenza-like illness.</p><p>PH1N1. H1N1pdm2009 virus-infected pregnant women.</p><p>a. HW vs ILI.</p><p>b. ILI vs PH1N1.</p><p>c. HW vs PH1N1.</p><p>d. HP vs PH1N1.</p><p>e. HW vs HP.</p><p>f. HP vs ILI.</p>±<p>Kruskal-Wallis test with Dunn’s multiple comparison post-test.</p><p>P values: * = p<0.05; ** = p<0.01; *** = p<0.001.</p><p>Distribution of leukocytes in the blood.</p