1 research outputs found
Comparative Nontargeted Profiling of Metabolic Changes in Tissues and Biofluids in High-Fat Diet-Fed Ossabaw Pig
Typical
clinical biomarker analyses on urine and plasma samples
from human dietary interventions do not provide adequate information
about diet-induced metabolic changes taking place in tissues. The
aim of this study was to show how a large-scale nontargeted metabolomic
approach can be used to reveal metabolite groups for generating new
hypotheses of obesity-related metabolic disturbances produced in an
animal model. A large spectrum of metabolites in the semipolar region,
including small water-soluble molecules like betaine and dihydroxyindole,
and a wide range of bile acids as well as various lipid species were
detected. The high-fat diet influenced metabolic homeostasis of Ossabaw
pigs, especially the lipid metabolome, throughout all the analyzed
sample types, including plasma, urine, bile, liver, pancreas, brain
cortex, intestinal jejunum and proximal colon. However, even dramatic
metabolic changes in tissues were not necessarily observed in plasma
and urine. Metabolite profiling involving multiple sample types was
shown to be a feasible method for the examination of a wide spectrum
of metabolic species extending from small water-soluble metabolites
to an array of bile acids and lipids, thus pointing to the pathways
of metabolism affected by the dietary treatment