RegFus: A toolbox for distributed multi-view image registration and fusion

Multi-view and multi-tile light (sheet) microscopy techniques produce large image datasets that are unaligned in space, time and channels. We present a collection of algorithm implementations and scripts to register and fuse such datasets in 2-5d, including e.g. group registration and multi-view deconvolution. Based on the scientific python stack and leveraging dask for chunked and distributed image processing, RegFus enables the efficient reconstruction of large multi-view datasets with low memory requirements, allowing to make use of GPUs and distributed computing. Visualization in napari helps to guide and troubleshoot the reconstruction process.</p

Photoresponsive and Magnetoresponsive Graphene Oxide Microcapsules Fabricated by Droplet Microfluidics

Fluid compartmentalization by microencapsulation is important in scenarios where protection or controlled release of encapsulated species, or isolation of chemical transformations is the central concern. Realizing responsive encapsulation systems by incorporating functional nanomaterials is of particular interest. We report here on the development of graphene oxide microcapsules enabled by a single-step microfluidic process. Interfacial reaction of epoxide-bearing graphene oxide sheets and an amine-functionalized macromolecular silicone fluid creates a chemically cross-linked film with micronscale thickness at the surface of water-in-oil droplets generated by microfluidic devices. The resulting microcapsules are monodisperse, mechanically resilient, and shape-tunable constructs. Ferrite nanoparticles are incorporated via the aqueous phase and enable microcapsule positioning by a magnetic field. We exploit the photothermal response of graphene oxide to realize microcapsules with photoresponsive release characteristics and show that the microcapsule permeability is significantly enhanced by near-IR illumination. The dual magnetic and photoresponsive characteristics, combined with the use of a single-step process employing biocompatible fluids, represent highly compelling aspects for practical applications

Photoresponsive and Magnetoresponsive Graphene Oxide Microcapsules Fabricated by Droplet Microfluidics

Fluid compartmentalization by microencapsulation is important in scenarios where protection or controlled release of encapsulated species, or isolation of chemical transformations is the central concern. Realizing responsive encapsulation systems by incorporating functional nanomaterials is of particular interest. We report here on the development of graphene oxide microcapsules enabled by a single-step microfluidic process. Interfacial reaction of epoxide-bearing graphene oxide sheets and an amine-functionalized macromolecular silicone fluid creates a chemically cross-linked film with micronscale thickness at the surface of water-in-oil droplets generated by microfluidic devices. The resulting microcapsules are monodisperse, mechanically resilient, and shape-tunable constructs. Ferrite nanoparticles are incorporated via the aqueous phase and enable microcapsule positioning by a magnetic field. We exploit the photothermal response of graphene oxide to realize microcapsules with photoresponsive release characteristics and show that the microcapsule permeability is significantly enhanced by near-IR illumination. The dual magnetic and photoresponsive characteristics, combined with the use of a single-step process employing biocompatible fluids, represent highly compelling aspects for practical applications