Estabelecimento de cultura de células em suspensão e identificação de flavonóides em Cordia verbenácea DC.

O trabalho teve como objetivos o estabelecimento de culturas de células em suspensão, extração, separação e identificação de flavonóides em extratos de folhas e de células em suspensão de Cordia verbenacea. Células dessa espécie, após terem sido subcultivadas três vezes no meio MS suplementado com 2,32 ?M de cinetina + 10,74 ?M de ANA a intervalos de 28 dias, apresentaram cinco estágios de crescimento: as fases lag, exponencial, linear, desaceleração e estacionária. O maior percentual de crescimento (37%) ocorreu no período exponencial entre o quarto e o décimo segundo dia e o menor (3%) na fase lag até o quarto dia. Para identificação de flavonóides, foram usados extratos submetidos à separação e purificação por CCD e CCL e os componentes obtidos submetidos à Espectroscopia de Ultravioleta, Espectrometria de Infravermelho e Massa e Ressonância Magnética Nuclear de Hidrogênio. Após as frações das amostras de folhas e células terem sido separadas pelo eluente ácido acético, foram identificados os componentes 7,4?-diidróxi-5?-carboximetóxi isoflavona e 7,4?-diidróxi-5?-metil isoflavona. Foi detectado maior concentração dessas substâncias nas células cultivadas in vitro.AMEIRA, O. A. [i.e. LAMEIRA, O. A.]. Disponível também on-line

Distillation methods affect the chemical composition of Varronia curassavica Jacq, essential oil?

The objective of this work was to evaluate the chemical composition of essential oil from Varronia curassavica Jacq. obtained by microwave (MI) and hydrodistillation (HD) extraction methods. The MI method tested three powers (500, 600, and 700W), three distillation times (20, 30, and 40 min.), and three water volumes (0, 25, and 50 mL per sample). The HD method tested three distillation times (100, 120, and 140 min.) and three water volumes (1.0, 1.5, and 2.0 L per 3-liter flask). The essential oils were analyzed by GC/MS-FID. The optimal condition for the essential oil extraction by the MI method was 700W for 40 min. (3.28%), regardless of the volume of water. In its turn, the best condition for essential oil extraction by the HD method was 120 min. with 1.0 L of water per flask (3.34%). The most abundant compounds for MI (700 W for 40 min. without water) were shyobunol (26.53%) and bicyclogermacrene (4.96%); and the most abundant compounds for HD (120 min. with 1.0 L of water/flask) were shyobunol (24.00%) and germacrene D-4-ol (10.23%). Methyl farnesoate (2E, 6E) and farnesyl acetate (2Z, 6E) were not detected in the essential oil extracted by HD; however, they were identified by the MI method. By increasing the distillation time and/or volume of water in HD, a reduction was observed for the content of the chemical compounds -elemene (from 1.23 to 0.97%), Ecaryophyllene (from 5.49 to 4.35%), -humulene (from 1.80 to 1.43%), alloaromadendrene (from 1.78 to 1.44%), bicyclogermacrene (from 5.63 to 4.55%), and germacrene D-4-ol (from 11.40 to 9.86%). Power, extraction time, and their interactions influenced the content of essential oil obtained by microwave extraction (MI). Within each power, the highest essential oil content was extracted at the longest distillation time (40 min.), except for 600W, where no significant difference was detected between 30 and 40 min. The optimal essential oil contents for both extraction methods were statically similar by the t-test for dependent samples. However, the MI method presents advantages, such as shorter distillation time and less energy and water consumption

Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers.

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Deficiências nutricionais no crescimento de Cordia verbenacea: altura e peso da matéria seca de planta.

Edição dos Resumos do 37º Congresso Brasileiro de Olericultura, Manaus, 1997. Resumo 017

Toxicity and repellency of the essential oil from Lippia gracilis to the coconut mite Aceria guerreronis (Acari: Eriophyidae).

The essential oil (EO) from different genotypes of Lippia gracilis Schauer (Verbenaceae) present two distinctive chemotypes containing either thymol or its isomer carvacrol as major compounds, both of which have proven bioactivity against several agricultural pests. Recently, we have shown that L. gracilis accession LGRA 106 and its major compound thymol are toxic and repellent against the coconut mite, Aceria guerreronis Keifer (Acari: Eriophyidae), a key pest of coconut plantations in Asia, Africa and America. Since intraspecific variation affects the chemical composition of EO and hence, its bioactivity, here we assessed the acaricidal and repellent effects of L. gracilis accession LGRA109 to A. guerreronis. Leaves of this accession contain carvacrol (49.35%) as major compound. The LC50 of the EO and of carvacrol estimated for A. guerreronis were 28.01 and 6.84 mg/mL, respectively. Carvacrol, at its LC50, as well as the EO and carvacrol at their LC99, repelled the mite. Our results indicated that carvacrol and the EO of L. gracilis accession LGRA109 were bioactive against A. guerreronis; however, the EO was less effective than its major compound

Genetic diversity of a native population of Myrcia ovata (Myrtaceae) using ISSR molecular markers.

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Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers.

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Análise molecular via ISSR de uma população nativa de Eplingiella fruticosa.

Edição Especial dos Anais do 3° Simpósio da Rede de Recursos Genéticos Vegetais do Nordeste, Aracaju, out. 2017