Additional file 1: of A lifelong study of a pack Rhodesian ridgeback dogs reveals subclinical and clinical tick-borne Anaplasma phagocytophilum infections with possible reinfection or persistence

Table S1. Tabulated quarter year life events and serological, molecular, haematological and clinical data from six dogs, 1998 throughout 2009. Life histories of the six Rhodesian ridgeback dogs described per quarter year from 1998 through 2009. For each dog 8 columns were annotated: a Life events and surgical interventions, medications; b Rectal temperature taken in the veterinary clinic; c Clinical observations especially bleeding tendencies and immunological derived symptoms; d Other clinical observations as lameness, pain, fatigue, tumors; e Thrombocyte counts; f Erythrocyte counts or form observations; g White blood cell counts; h Serological and molecular (DNA) detection of infection. Abbreviations: N, neutrophilia; n, neutropenia; L, lymphocytosis; l, lymphopenia; M, monocytosis; m, monocytopenia; E, eosinophilia; e, eosinopenia; Morula, morulae detected in neutrophils; P/P both positive; P/N, serological positive/molecular negative; N/P, serological negative/molecular positive; N/N, both negative; -/N, -/P, N/-, P/-, -/-, either one or both not performed. Note: No abbreviation is given for normal cell count. (XLS 176 kb

Percentage of positive samples for <i>C</i>. <i>burnetii</i> DNA per week from March–September 2011.

<p>Percentage of positive samples for <i>C</i>. <i>burnetii</i> DNA per week from March–September 2011.</p

Univariable associations between the presence of <i>C</i>. <i>burnetii</i> in PM10 and spatial, temporal and spatial-temporal characteristics.

<p>Univariable associations between the presence of <i>C</i>. <i>burnetii</i> in PM10 and spatial, temporal and spatial-temporal characteristics.</p

Descriptive statistics of the spatial, temporal and spatial-temporal characteristics.

<p>Descriptive statistics of the spatial, temporal and spatial-temporal characteristics.</p

Overview of monthly averaged outdoor temperature and pattern of kidding season (estimated monthly percentages of goats born in the Netherlands based on the paper of Hermans et al [22]).

<p>Overview of monthly averaged outdoor temperature and pattern of kidding season (estimated monthly percentages of goats born in the Netherlands based on the paper of Hermans et al [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151281#pone.0151281.ref022" target="_blank">22</a>]).</p

Significant temporal and spatial associations to the presence of <i>C</i>. <i>burnetii</i> in PM10, mutually adjusted in the multivariable model.

<p>Significant temporal and spatial associations to the presence of <i>C</i>. <i>burnetii</i> in PM10, mutually adjusted in the multivariable model.</p

Estimated temperature profiles inside the dunghill at Farm A on 4th November 2009.

<p>Cases for Q_prod = 0 (only conduction, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121355#pone.0121355.e008" target="_blank">Equation 3</a>) and for Q_prod = 50 W/m³ and λ = 2 W/m∙K (conduction and heat production, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121355#pone.0121355.e003" target="_blank">Equation 2</a>) are shown. The λ value of wet soil is taken [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121355#pone.0121355.ref020" target="_blank">20</a>], the value for Q_prod was estimated based on heat transfer calculations using the outdoor air temperature on 4^th November 2009.</p

<i>Coxiella burnetii</i> PCR results in manure from two dairy goat farms.

<p>Number of <i>C</i>. <i>burnetii</i> positive samples categorized in manure location per farm. The category ‘Not determined’ reflects samples for which no signals were observed in the internal control, or <i>C</i>. <i>burnetii</i> targets.</p><p><i>Coxiella burnetii</i> PCR results in manure from two dairy goat farms.</p

Outside and dunghill temperatures during the experiment.

<p>In Fig 3a, temperatures in the core (dark blue) and shell (red) of the dunghill on farm A are presented. In Fig 3b, temperatures in the core (dark blue) and shell (red) of the dunghill on farm B are presented. For both farms, the average (yellow), the minimum (turquoise) and the maximum (purple) outside air temperature in Eindhoven, the Netherlands (<a href="http://www.knmi.nl" target="_blank">www.knmi.nl</a>) during the experiments are shown. All temperatures are in degree Celsius.</p

Distribution of manure and incidences of human Q fever patients.

<p>In Fig. 2a, twelve dairy goat farms with abortion waves caused by <i>Coxiella burnetii</i> in 2008 and/or 2009 (case farms; red dots), and 24 dairy goat farms without notified abortion waves caused by <i>C</i>. <i>burnetii</i>, bulk tank milk (BTM) PCR negative results between 2009 and 2014, and BTM ELISA negative results in 2008 from which records of manure distribution were available (controls; green dots), as well as incidences (number of cases per 100,000 residents) of human Q fever patients (the darker area, the more human Q fever patients) are presented. In Fig. 2b, distributions of manure from case farms outside a radius of ten km around case farms to four-digit postcode areas (dark colored) are presented. In Fig. 2c, distributions of manure from control farms outside a radius of ten km around case farms to four-digit (dark colored) are presented.</p