Anatomy of the <i>C. elegans</i> as revealed by visOCM.

<p><b>(A, B)</b><i>En face</i> projections at two different depths, and <b>(C)</b> side view at the location highlighted in <b>(B)</b>. Scale bars indicate 50 μm. <b>(D)</b> Top: A 3D rendered model of the head with the pharynx highlighted in green. Bottom: Maximum-intensity projection through the entire animal’s head. <b>(E)</b> <i>En face</i> view (top) and corresponding transverse sections (bottom), with the lumen of the intestine highlighted in yellow. <b>(F)</b> Zoom regions of the reproductive system showing germ cells, oocytes, spermatheca, embryos and the vulva. The 3D sub-micrometer resolution and the intrinsic contrast of our technique enable a clear and detailed visualization of tissue structures down to the sub-cellular level (see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181676#pone.0181676.s003" target="_blank">S2 Video</a>).</p

Schematic of the visOCM setup for <i>C. elegans</i> imaging.

<p>Light from a laser source with a broad spectrum in the visible range (<b>A</b>, inset) is collimated by lens L1 and split by beam-splitter BS1 into a sample (green) and reference (blue) arm. In the sample arm, the axicon lens generates a Bessel-like illumination beam which is then guided to the tube lens (TL) and objective by the X-Y galvo-scanner unit. The back-reflected light (red) from the sample (<b>B</b>, inset) is recombined with the reference arm by beam-splitter BS2 and focused by L2 into the detection fiber. Finally, the spectrometer (<b>C</b>, inset), records the interference pattern which is processed to yield a depth profile of the <i>C. elegans</i> structure. The data processing steps are illustrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181676#pone.0181676.s001" target="_blank">S1 Fig</a>. Scale bars: 25 μm.</p

Visualization 3: Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Scan in depth of a brain slice acquired with visOCM containing amyloid plaques. Scalebar: 50 um Originally published in Biomedical Optics Express on 01 July 2017 (boe-8-7-3343

Visualization 2: Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Scan in depth of a brain slice acquired with visOCM containing a large vessel caliber. Scalebar: 50 um Originally published in Biomedical Optics Express on 01 July 2017 (boe-8-7-3343

Media 1: Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Originally published in Optics Express on 10 September 2012 (oe-20-19-21385