Clonagem molecular da proteína core do vírus da hepatite c em sistemas de expressão procarionte e eucarionte / Molecular cloning of the hepatitis c virus core protein in procaryionte and eukaryonite expression systems

A maioria dos casos de Hepatite C são assintomáticos e os sintomas aparecem quando a doença já apresenta comprometimento hepático. O presente trabalho objetivou a produção e purificação da proteína Core do vírus da Hepatite C (HCV) em sistema procarionte e sua subclonagem em vetores de expressão eucarionte. A bactéria E. coli foi selecionada para realizar a síntese da proteína de interesse, sendo esta purificada por cromatografia de afinidade e detectada por western blotting. Além disso, a sequência de interesse foi recombinada em plasmídeos para direcionamento a subcompartimentos da célula vegetal, sendo estes confirmados por PCR. Os processos realizados tiveram êxito e a posterior expressão nos diferentes compartimentos celulares possibilitará o melhor rendimento para a produção de imunógenos e viabilizar a produção de kit diagnóstico

Prevalence of COVID-19 in children, adolescents and adults in remote education situations in the city of Fortaleza, Brazil

Objectives: A retrospective study was conducted to identify the prevalence of COVID-19 through serology and RT-PCR in children, adolescents and adults. A database of the COVID-19 Tracking Program in school children was used. Methods: The data comprised sociodemographic and clinical variables, results of serological tests (IgM and IgG), and real-time-polymerase chain reaction (RT-PCR) results of IgM-positive individuals. The statistical analysis was performed with a 5% significance level. Results: Among 423 children, 107 (25.3%) exhibited seroprevalence with IgG, IgM or IgG/IgM; among 854 adolescents, 250 (29.2%) had positive serology; and among 282 adults, 59 (20.9%) were positive. The frequency of positivity on RT-PCR for SARS-CoV-2 was 3.5%, 3.6% and 6.0% in children, adolescents and adults, respectively. Children had a lower incidence of symptoms than adolescents (p = 0.001) and adults (p = 0.003); the most frequent were fever, ageusia, anosmia, headache, dry cough, sore throat, muscle pain, runny nose, dyspnoea, and diarrhoea. Conclusions: The prevalence rate for all groups was 26.7% in serology and 4.04% in RT-PCR. Children had lower rates of IgM and fewer symptoms compared with adolescents and adults. The data suggest the potential for transmissibility in all age groups