Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema

This study aimed to investigate antioxidant and anti-inflammatory activities of a commercial product of noni (Morinda citrifolia) juice. Carrageenan-induced rat paw edema was employed as inflammatory model. One control and three experimental groups were formed. Experimental groups were administered noni juice alone, noni juice+carrageenan, and carrageenan alone. Oxidant and antioxidant capacity were determined by d-ROMs test and BAP test, respectively. Plasma concentrations of endothelin-1 and leptin were measured by ELISA. Measurements were performed at zero time and 2nd hour of inflammation. Oxidant capacity decreased in noni-received groups at 2nd hour (p=0.019). Antioxidant capacity of the group which received noni alone was found to be higher at 2nd hour (p=0.036). Plasma concentrations of endothelin-1 and leptin were notably lower in noni-received groups (p=0.001 and p=0.021, respectively). The results show that the commercial noni juice investigated has pronounced antioxidant and anti-inflammatory activities

Anticancer effect of a novel palladium-saccharinate complex of terpyridine by inducing apoptosis on ehrlich ascites carcinoma (EAC) in balb-C mice

Background/Aim: [Pd(sac)(terpy)](sac)•4H2O (sac=saccharinate and terpy=2,2′:6′,2-terpyridine) is newlysynthesized palladium(II) (Pd) complex. We investigated the antiproliferative and apoptotic effects of this complex on Ehrlich ascites carcinoma (EAC). Materials and Methods: EAC cells were administered to 33 Balb/c mice. Mice were divided randomly into four groups: control, cisplatin, Pd(II) complex and paclitaxel. Control group animals received 0.9% NaCl; other groups received treatments cisplatin, Pd(II) complex and paclitaxel on days 7 and 12. At day 14, animals were sacrificed. Expression of active caspase-3, p53 and proliferating cell nuclear antigen (PCNA) was investigated and apoptosis was evaluated by terminal deoxynucleotidyltransferase (TdT)-mediated nick-end labelling (TUNEL) technique. Results: Expression of p53 and PCNA were found to be decreased (p<0.0001), cells with active caspase-3 and TUNEL-positive cells were found to be increased (p<0.0001) in all treatment groups. Conclusion: Like cisplatin and paclitaxel, this Pd(II) complex has a strong anticancer activity against EAC by inducing apoptosis and suppressing proliferation in vivo.Istanbul University (UDP-47082

ATP testi, MTT testinin aksine, meme kanseri hücrelerinde antrasiklin-bazlı tedavinin histon deasetilaz i̇nhibitörü ile kombinasyonunun yarattıǧı daha i̇leri düzeydeki sitotoksisiteyi tespit edebilmektedir

Purpose: It has been investigated that whether or not the combination of valproic acid (a histone deacetylase inhibitor) with anthracycline-based chemotherapy (FEC: 5-fluorouracil+epirubicine+ cyclophosphamide) would change the cytotoxic effects of FEC in breast cancer cells. Methods: The effect of valproic acid and its combination with FEC has been tested on MDA-MB-231 and MCF-7 human breast cancer cell lines. Anti-growth effects of treatments were determined by the MTT and ATP assays, while the detection of apoptosis was performed by the caspase-cleaved cytokeratin 18 assay. Results: Valproic acid treatment had anti-growth effect on the cell lines used at clinically achievable dose (0.6 mM). According to the MTT assay, the combination of valproic acid with different doses (50-200% Test Drug Concentration) of FEC did not result in any significant change over FEC-only treatment in both cell lines. However, according to the ATP assay, there has been found that the combination of 100% Test Drug Concentration FEC with valproic acid yielded more efficacy compared to FEC-alone. FEC induced the apoptosis in MCF-7 cells but the addition of valproic acid to FEC did not enhance apoptosis. Conclusion: According to the ATP assay, the use of valproic acid at the clinically achievable dose (0.6 mM) with different doses of FEC further increased the cytotoxic effect of FEC. However, this effect was not observed in the MTT assay. A caution should therefore be taken on the evaluation of the cytotoxic effect of valproic acid in cell lines