Bland-Altman bias plots comparing Bam-W repeated- and LMP2 single- sequence qPCRs on whole blood extracts of clinical samples.

<p>Values having EBV DNA load equal or higher than LOD of LMP2 (A) and Bam-W (B) in-house qPCR assays were compared with the initial clinical values (equal or higher than LOD) performed by EBV R-gene commercial kit, and with each other (C). Dotted lines represent the borders of 95% CI and 0 point.</p

Bland-Altman bias plots for three different quantitative EBV DNA real-time PCR assays.

<p>Ten serial dilutions of Raji and B95.8 cell line supernatants were tested for EBV DNA quantification by LMP2 and EBV R-gene qPCRs (A). The same samples were tested by Bam-W qPCR compared with R-gene (B) and LMP2 qPCRs (C).</p

Biases, standard deviations and comparison of biases (P values) of Bland-Altman curves on different EBV strains.

<p>Biases, standard deviations and comparison of biases (P values) of Bland-Altman curves on different EBV strains.</p

Cohen’s kappa agreements of EBV DNA between qualitative results obtained from whole blood clinical sample of R-gene commercial kit and laboratory in-house Bam-W and LMP2 qPCR assays.

<p>Cohen’s kappa agreements of EBV DNA between qualitative results obtained from whole blood clinical sample of R-gene commercial kit and laboratory in-house Bam-W and LMP2 qPCR assays.</p

Probit regression analysis determining the limit of detection (the lowest viral load detectable in 95% of the time, corresponding to probit value 6.65) of Bam-W repeated- and LMP2 single- sequence qPCRs.

<p>A series of 7 and 6 EBV concentrations for Bam-W and LMP2 qPCRs, respectively, were prepared by spiking 1st WHO international standard in EBV DNA negative plasma. Each dilution was tested in 4 to 62 replicates. The number of replicates, positivity rate and corresponding probit value of each dilution is presented in supporting <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183856#pone.0183856.s002" target="_blank">S2 Table</a>.</p