Analysis of Capsaicinoids in Chilli Sauce with Ultra Fast Liquid Chromatography

Objective:In current study, quantification of the capsaicinoids in chilli sauces based on a sensitive ultra fast liquid chromatography method and derivatization with dansyl chloride (DNS-Cl) was described. Capsaicinoids are biosynthesized as secondary metabolites by chilli sauces. The major components of capsaicinoids are capsaicin (CPS) and dihydrocapsaicin (DCPS).Methods:Phenol groups within the CPS and DCPS are suitable for derivatization reaction using DNS-Cl (chemically named as 5-(dimethylamino) naphthalene-1-sulfonyl chloride) at pH 10 with 0.5 M sodium bicarbonate which leads formation of a derivative highly fluorescent properties that can be measured at 520 nm following excitation at 360 nm wavelength. Separation of the compounds was conducted on a chromatographic system having a mobile phase formed by a combination of acetic acid (0.5 M, pH 7.0 with NaOH) solution and acetonitrile under solvent programming on a consistent flow rate of 0.4 mL. min-1 using a C18 column.Results:Method validation was evaluated as per the regulations described in International Conference on Harmonization Guidelines. The calibration graph for CPS and DCPS was linear between 0.2 and 200 µg mL-1.Conclusion:The proposed analytical procedure represents a simple, time and cost effective method with a suitable selectivity regarding quantification of capsaicinoids in chilli sauces

Analysis of Racecadotril in Pharmaceutical Formulations Using Ultra Performance Liquid Chromatography (UPLC) Method

Objective:In this study, a simple and selective ultra-performance liquid chromatography method for the determination of racecadotril in pharmaceutical dosage forms has been developed.Methods:The method is in reverse phase C18 column; based on the chromatographic analysis of racecadotril with acetonitrile-water (70: 30, v/v) mobile phase at a flow rate of 0.7 mL/minimum.Results:The most suitable wavelength for racecadotril has been determined 220 nm. The linearity range for racecadotril was found 5-100 μg/mL.Conclusion:This method was applied to the analysis of racecadotril drug substance in tablets. The results were evaluated statistically. The developed method is easy, precise and repeatable and can be used safely in racecadotril pharmaceutical dosage forms analysis