RAGE signaling contributes to neuroinflammation in infantile neuronal ceroid lipofuscinosis

AbstractPalmitoyl-protein thioesterase-1 (PPT1) deficiency causes infantile neuronal ceroid lipofuscinosis (INCL), a devastating childhood neurodegenerative storage disorder. We previously reported that neuronal apoptosis in INCL is mediated by endoplasmic reticulum-stress. ER-stress disrupts Ca2+-homeostasis and stimulates the expression of Ca2+-binding proteins. We report here that in the PPT1-deficient human and mouse brain the levels of S100B, a Ca2+-binding protein, and its receptor, RAGE (receptor for advanced glycation end-products) are elevated. We further demonstrate that activation of RAGE signaling in astroglial cells mediates pro-inflammatory cytokine production, which is inhibited by SiRNA-mediated suppression of RAGE expression. We propose that RAGE signaling contributes to neuroinflammation in INCL

Surface and Ground Water Pollution in Bangladesh: A Review

Environmental analysis does not become properly meaningful unless follow the standard procedure in sampling, preserving and also in analysis. This study investigates different studies on surface and groundwater pollution and discusses their findings along with impacts on environment, human health and aquatic ecosystem demolition. Fresh water is a vital need for drinking, household, agricultural and industrial use. Fresh water availability is shrinking worldwide due to imprudent use. Abound availability of fresh water in Bangladesh is now polluting due to unwise dumping of industrial, household, agricultural and municipal wastes. Water body in urban arena is severely polluted rather than rural part. The over concentration of DO, BOD, COD, EC, TDS, Cr, Cd, Pb was observed in Dhaka region. The highest arsenic concentration was found in the ground water of Lakshimpur. The main sources of pollution in industrial cities are anthropogenic sources like untreated industrial effluent and municipal wastes whereas in rural part the main sources of pollution are agricultural and naturogenic. Transboundary impact also intensifies some river water pollution. Government approved the policies, ordinance, acts and laws however, due to lack of proper implementation and monitoring water pollution problem increasing day by day

THE SPLICEOSOMAL PROTEIN SnRNP F BINDS TO BOTH U3 AND U14 CLASS OF snoRNA IN Giardia lamblia

Small nuclear Ribonucleo Protein F (snRNP F) is a spliceosomal protein that binds with U1, U2, U4/U6 and U5 small nuclear RNA (snRNA) to form spliceosomal complexes responsible for pre mRNA processing. This study reports the unusual interaction of giardial snRNP F with small nucleolar RNAs (snoRNA) that are responsible for pre rRNA processing. Electrophoretic Mobility Shift Assay was used to demonstrate the interaction of this protein with U3 and U14 class snoRNA of the early branching eukaryote Giardia lamblia. It was also evident from our study that snRNP F in Giardia is evolutionary distinct from its other eukaryotic orthologues

Non-Lorentzian Kač-Moody Algebras

International audienceWe investigate two dimensional (2d) quantum field theories which exhibit Non- Lorentzian Kač-Moody (NLKM) algebras as their underlying symmetry. Our investigations encompass both 2d Galilean (speed of light $c \rightarrow \infty$) and Carrollian ($c \rightarrow 0$) CFTs with additional number of infinite non-Abelian currents, stemming from an isomorphism between the two algebras. We alternate between an intrinsic and a limiting analysis. Our NLKM algebra is constructed first through a contraction and then derived from an intrinsically Carrollian perspective. We then go on to use the symmetries to derive a Non-Lorentzian (NL) Sugawara construction and ultimately write down the NL equivalent of the Knizhnik Zamolodchikov equations. All of these are also derived from contractions, thus providing a robust cross-check of our analyses

Evaluation of Micromechanical Properties of Carbon/Carbon and Carbon/Carbon-Silicon Carbide Composites at Ultralow Load

2D carbon fiber (C-fiber)-reinforced carbon/carbon (C/C) composites were prepared by vacuum infiltration with coal-tar pitch followed by carbonization and graphitization in inert atmosphere. Liquid silicon infiltration was done in controlled atmosphere at 1600 degrees C to convert the matrix carbon of the C/C composites into silicon carbide. The 2D C/C and carbon/carbon-silicon carbide (C/C-SiC) composites had density of similar to 1.65 and similar to 2.32 g/cm3, respectively with corresponding flexural strength of similar to 70 and similar to 169 MPa, respectively. The local mechanical properties like hardness, Young's modulus, contact pressure, relative stiffness, relative spring back, and indentation energies of the two composites under different loading conditions were measured at an ultra low load of 10 mN using a nanoindentation instrument with a Berkovich indenter. The scatter in the data was treated in terms of the two-parameter Weibull statistical analysis. The maximum characteristic Young's modulus (similar to 16 GPa) and hardness (1.20 GPa) was obtained for the C/C-SiC composites in parallel direction of fabric stacking. The elastic rebounce was also the maximum (0.77) for the C/C-SiC composites when loaded in parallel direction of fabric stacking. The extent of structural anisotropy was higher in the C/C-SiC composite than that of the C/C composite

THE SPLICEOSOMAL PROTEIN SnRNP F BINDS TO BOTH U3 AND U14 CLASS OF snoRNA IN Giardia lamblia

Small nuclear Ribonucleo Protein F (snRNP F) is a spliceosomal protein that binds with U1, U2, U4/U6 and U5 small nuclear RNA (snRNA) to form spliceosomal complexes responsible for pre mRNA processing. This study reports the unusual interaction of giardial snRNP F with small nucleolar RNAs (snoRNA) that are responsible for pre rRNA processing. Electrophoretic Mobility Shift Assay was used to demonstrate the interaction of this protein with U3 and U14 class snoRNA of the early branching eukaryote Giardia lamblia. It was also evident from our study that snRNP F in Giardia is evolutionary distinct from its other eukaryotic orthologues

Anatomy of the dorsal default-mode network in conduct disorder:Association with callous-unemotional traits

We recently reported that emotional detachment in adult psychopathy was associated with structural abnormalities in the dorsal 'default-mode' network (DMN). However, it is unclear whether these differences are present in young people at risk of psychopathy. The most widely recognised group at risk for psychopathy are children/adolescents with conduct disorder (CD) and callous-unemotional (CU) traits. We therefore examined the microstructure of the dorsal DMN in 27 CD youths (14-with/13-without CU traits) compared to 16 typically developing controls using DTI tractography. Both CD groups had significantly (p < 0.025) reduced dorsal DMN radial diffusivity compared to controls. In those with diagnostically significant CU traits, exploratory analyses (uncorrected for multiple comparisons) suggested that radial diffusivity was negatively correlated with CU severity (Left: rho = -0.68, p = 0.015). These results suggest that CD youths have microstructural abnormalities in the same network as adults with psychopathy. Further, the association with childhood/adolescent measures of emotional detachment (CU traits) resembles the relationship between emotional detachment and network microstructure in adult psychopaths. However, these changes appear to occur in opposite directions - with increased myelination in adolescent CD but reduced integrity in adult psychopathy. Collectively, these findings suggest that developmental abnormalities in dorsal DMN may play a role in the emergence of psychopathy