Estimates of Evolutionary Divergence over Sequence Pairs between HBV/D subgenotypes (D1–D9) and Indian HBV/D4 (IndD4).

<p>Genetic distances of Indian HBV/D4 (IndD4) with other subgenotypes of D are indicated in bold and the genetic distance between IndD4 and reported D4 is enclosed within a box.</p><p>Estimates of Evolutionary Divergence over Sequence Pairs between HBV/D subgenotypes (D1–D9) and Indian HBV/D4 (IndD4).</p

Conserved amino acid residues in four open reading frames of Indian HBV/D4 (IndD4) isolates compared with the previously reported residues at the same position in different subgenotypes of D (D1–D9).

<p>Unique residues of IndD4 are underlined. The major amino acids are indicated in <b>bold</b>. ^aThe conserved domains of the open reading frame of HBV polymerase are indicated as follows: tp, terminal protein domain; sp, spacer region; rt, reverse transcriptase domain; rh, RNase H region. *, ** the overlapping surface and polymerase mutations</p><p>Conserved amino acid residues in four open reading frames of Indian HBV/D4 (IndD4) isolates compared with the previously reported residues at the same position in different subgenotypes of D (D1–D9).</p

Primers used in this study.

<p>**  =  nucleotide [nt] positions are given according to HBV sequence with accession no. AF121242 obtained from GenBank.</p>ψ<p>Gunther S <i>et al</i>. J. Virol. 1995; 69: 5437–44.</p><p>Primers used in this study.</p

Estimation of divergence times of non-recombinant HBV subgenotypes D1–D7 including Indian HBV/D4 (IndD4).

<p>The tree was constructed by the DNAMLK program of the PHYLIP package, using indel-free HBV sequences of different subgenotypes of D, derived from GenBank, along with 12 IndD4 sequences. Reference sequences are indicated by their specific subgenotypes followed by accession numbers and the IndD4 sequences are indicated by respective isolate numbers beginning with “TP.”</p