Cats blood types: Distribution of blood types in respect of cats breed and geographical location

WOS: 000254709600014Cats have three blood types called as A, B and AB. Blood typing has been performed in both non-pedigree and purebred cat populations in various parts of the world. There are noticeable geographical variation in distribution of blood types, which is important in clinical practice in preventing neonatal isoerythrolysis (NI) and acute hemolytic transfusion reactions. This reaction associates with severe hemolytic anemia, anaphylactic shock and even death. Severity of reactions associate with titres of naturally occurring alloantibodies, found in cat plasma, against the heterelogous blood types. Type A is the most common blood group observed in cats. In contrast, type AB cats are rare, with a frequency of less than one per cent in most of the non-pedigree and purebred cat populations studied worldwide. Present paper reviews current understanding of blood types, role of geographic localization in the distribution of blood types and their importance in feline transfusion medicine

Clinical aspects of blood transfusion in cats

WOS: 000265357000014The safe use of transfusion therapy requires proper knowledge on blood groups, blood typing, donor selection, blood collection, transfusion protocols and adverse transfusion reactions. Type B cats have high titres of naturally occurring anti-A isoagglutinins and type A cats have generally low titred anti-B isoagglutinins in their plasma. The severity of incompatibility reactions in blood transfusion is proportional to the alloantibody titres of the recipient blood. Transfusion of type B blood to type A cats may develop a mild transfusion reactions. In contrast, administration of type A blood to type B cats can cause potentially fatal acute hemolytic transfusion reactions. Adverse transfusion reactions can be avoided by typing donor and recipient cats before transfusion and by administering only matched blood. Due to improvement in feline blood typing, collecting and administration procedures, there has been a notable increase in the blood transfusion therapy in these animals. Present paper reviews current understanding of the pactical aspects of transfusion therapy in cats

The Effects of Low Doses of FSH and Season on the in Vitro Maturation of Bovine Oocytes

KARASAHIN, Tahir/0000-0003-2358-0389WOS: 000360810100290

The effect of oleic and linoleic acids on in vitro bovine embryonic development and embryo quality

KARASAHIN, Tahir/0000-0003-2358-0389WOS: 000353338100006The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 mu M). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 mu M) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morulablastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.TAGEM, TurkeyGida Tarim Ve Hayvancilik BakanligiThis study was financed under a project supported by TAGEM, Turkey. This manuscript was prepared as a PhD thesis accepted by the Kirikkale University Graduate School of Health Sciences

Vitrification of Bovine Embryos Developed in Vitro in Culture Medium Containing Oleic and Linoleic Acids

KARASAHIN, Tahir/0000-0003-2358-0389WOS: 000360810100289

The hair characteristics of Turkish Angora and Van cats

WOS: 000302964900001The primary aim of this study was to determine the hair characteristics of Turkish Angora and Van cats, and also to see if there were seasonal, sex, age, or eye color effects on the traits measured. A total of 41 cats, 26 Turkish Angora and 15 Turkish Van, were used. The ages of the cats ranged from 1 to 8 years old. Analyzed traits included fiber diameter (FD), fiber lengths of hauteur (H) and barbe (B), fiber tenacity (T), and elongation (EL). Terms included in the statistical models were breed, season, sex, age, and eye colors of the cats and 2-way interactions that had P-values of less than 0.2 among the main effects. The difference in the FD of the Turkish Angora (23.5 mu m) and Van (25.6 mu m) cats was not significant (P = 0.0579). The H (27.5 versus 20.1 mm), B (33.1 versus 23.4 mm), and EL (33.8% versus 24.0%) values were all greater (P < 0.0001) for the Turkish Angora cats, while only the T value (12.1 versus 7.6 g/den) was greater (P = 0.0001) for the Turkish Van cats. Significant phenotypic correlations were found between FD and T (0.36, P = 0.0191), between H and B (0.98, P < 0.0001), between H and T (-0.58, P < 0.0001), between H and EL (0.31, P = 0.0480), between B and T (-0.56, P = 0.0001), and between B and EL (0.34, P = 0.0315). The present study defines the hair characteristics of the Turkish Angora and Van cats. These results may help to understand the phenotypes of these cats better and could also be a source for further studies

Body Mass Index and Different Body Measurements of Turkish Angora and Van Cats in Two Different Locations

WOS: 000274143000024The aims of the present study were to compare the body mass index and some other body measurements of the Turkish Angora (n = 20) and Van (n = 16) cats in two different locations (Ankara Zoo = AOC and the Faculty of Veterinary Medicine, Kirikkale University = KKU) and also to determine the prevalence of overweight or obesity in this feline population. The effects of gender, age, location and eye colors were also investigated on the traits measured. The age of the cats ranged from 1-8 years old. Analyzed traits included Body Mass Index (BMI), live Body Weight (BW), Wither Height (WH), Body Length (BL), Head Circumference (HC), Body Condition Score (BCS). None of the cats in this study were found overweight or obese. The BMI of Turkish Van cats was greater than the BMI of Turkish Angora cats. The BW of Turkish Van cats tended to heavier than the Turkish Angora cats but the difference was not significant. The WH, BL and the HC values for both cats were similar. The BCS of Turkish Van cats was significantly greater than the BCS of Turkish Angora cats in KKU. Significant phenotypic correlations were found between the BW and the WH and the BL and the HC and the BMI and the BCS; between the WH and the BL and the HC; between the BL and the HC and the BCS; between the HC and the BCS; between the BMI and the BCS. The present study more clearly defines some body traits of the Turkish cats and may help to understand these invaluable cats better

Effects of cadmium, lead and mercury on progesterone synthesis in cultured bovine luteal cells

WOS: 000379514000276

Effects of cholesterol and cAMP on progesterone production in cultured luteal cells isolated from pseudopregnant cat ovaries

WOS: 000269418700024PubMed: 19135321The present study was designed to incubate luteal cells isolated from pseudopregnant cats and to investigate the effects of cholesterol and cAMP on luteal progesterone production. Corpora lutea were collected from the cats on days 10 and 15 of pseudopregnancy. Luteal cells were isolated from the ovaries by collagenase digestion. Steroidogenic luteal cells were stained for 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity. Cells (2 x 10(4)) staining positive for 3 beta-HSD were cultured for up to 7 days. The cells were treated with 22(R)-hydroxycholesterol (22R-HC) and dibutyryl cyclic AMP (dbcAMP) on days 1, 3 and 7. Treatment of cells with 22R-HC resulted in a dose-dependent increase (p < 0.001) in progesterone production. When 22R-HC was used at a concentration of 10 mu g/ml, it resulted in 2.7- and 5.1-fold increases in progesterone production on days 3 and 5, respectively. When the dose was doubled (20 mu g/ml), treated cells produced four times more progesterone on days 3 and 7, and three times more on day 5. By day 7, progesterone production increased up to 9.1 times more than the control. Incubation of cells with both concentrations of dbcAMP (0.1 mM and 1 mM) resulted in significant stimulations of progesterone on days 5 and 7 (p < 0.001). However, on day 3, only higher doses of dbcAMP (1 mM) resulted in significant stimulation (p < 0.05). Progesterone production was increased up to 2- and 2.9-fold of the control when cells were treated with lower concentration of dbcAMP (0.1 mM) on days 5 and 7. respectively. Incubation of cells with 1 mM concentrations of dbcAMP induced a 3.2-fold increase on day 5 and a 5-fold increase on day 7. In conclusion, a successful incubation was performed for long-life culturing of luteal cells collected from pseudopregnant cats. The method works well and allows for optimal growth and development of cells in the culture. The present study also demonstrated that incubating cat luteal cells with 22R-HC and dbcAMP induces a significant increase in luteal progesterone synthesis. (C) 2008 Elsevier B.V. All rights reserved.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-1050129]Financial support for the present project was provided by the Scientific and Technological Research Council of Turkey (TUBITAK). Project No.: TOVAG-1050129. The authors would like to thank Dr. Hakan Kalender for his assistance in collecting the ovaries from the cats

Evaluation of the Pre-Hemolytic Concentrations of beta-, Methyl-beta- and Dimethyl-beta-Cyclodextrin on Dog and Goat Erythrocytes

WOS: 000262560800010beta-Cyclodextrin (beta-CD) is a cage-like molecule consisting of seven glucose units and have appropriate size to form inclusion complexes with many hormones, vitamins and drugs. Due to having very poor aqueous solubility, beta-cyclodextrin is modified chemically for various applications. Experiments were conducted to examine effects of pre-hemolytic concentration of beta-cyclodextrin and its modified derivatives, methyl-beta-cyclodextrin (M beta-CD) and dimethyl-beta-cyclodextrin (DM beta-CD), on the osmotic fragility of dog and goat erythrocytes. Blood samples collected from healthy 10 dogs and 10 goats were analyzed within 5 h of collection. Erythrocyte suspensions were mixed with various concentrations of beta-CD, M beta-CD and DM beta-CD. The mixtures were then incubated for 30 min at 37 degrees C and osmotic fragility of erythrocytes was measured. Incubation of all 3 Cyclo Dextrins (CDs) with erythrocyte suspensions of both species, dog and goat, induced a dose dependent increase in the erythrocyte osmotic fragility (pM beta-CD>beta-CD. Hemolytic doses of these CDs were lower for goat erythrocytes than those of the dog erythrocytes. When beta-CD and its methylated derivatives are even used in pre-hemolytic concentrations, they might also reduce life span of erythrocytes. Due to importance of scientific knowledge on the cellular activity of cyclodextrins and growing number of its potential applications on diagnosis food preparations, our results may help to deal with the hemolytic activity of CDs for in vivo studies