Analisis Zerumbone Dalam Zingiber Zerumbet Dan Aktivitas Penghambatannya Terhadap Bakteri Mycobacterium Tuberculosis: Analysis of Zerumbone in Zingiber Zerumbet and Inhibitory Activity Against Mycobacterium Tuberculosis

Zerumbone has been reported for their several biological activities. In our interest to this compound, we have identified and analyzed its content in Zingiber zerumbet, a medicinal plant from Indonesian traditional medicine and investigated its inhibitory activity against Mycobacterium tuberculosis, a known infection bacteria of tuberculosis. Analysis of zerumbone was performed with densitometry to leave, rhizome, flower, and stem of Z. zerumbet which was extracted with various solvent system and extraction methods to determine the best method to isolate zerumbone from Z. zerumbet. Result showed that the highest zerumbone was in rhizome while was not observed in other part. Analysis with various solvent and extraction methods showed the highest yield of zerumbone can be extracted by n-hexane (maceration) and reflux extraction method (methanol). Furthermore, inhibitory activity of zerumbone against M. tuberculosis was tested using Lowenstein Jensen medium by counting the number of M. tuberculosis colony growth in medium. Resulted inhibitory activity of zerumbone at all test concentration (0.5, 0.1, 0.05, 0.01, 0.005%) with the growth of 10, 12, 14, 15, and 50 colonies of M. tuberculosis was observed, respectively. This is indicate that zerumbone can be used as an alternative choice for treatment tuberculosis in the future

Peningkatan Kualitas Guru Bimbingan dan Konseling melalui Penguasaan Teori Realita

IMPROVING THE QUALITY OF GUIDANCE AND COUNSELING TEACHERS THROUGH REALITY THEORY SKILLS. The importance of an effective quality counselor is the whole person of the counselor. This is indicated by the ability of the counselor's performance through the approach or theory of guidance and counseling. It aims to improve the quality of the counselor's performance through the reality therapy that can bring the full direction of the counselor's personality. Reality therapy directs the counselor's personality to be responsibility, honest (reality), sincere (right) to facilitate counselors in conducting guidance and counseling services. The embodiment of this is carried out through community service activities. The method of activity used is an online workshop (webinar) with steps, the first is the initial step (observation), the planning stage, the socialization stage, and the ongoing evaluation stage of the program. The community service activities were attended by 255 participants. The results obtained in community service activities are that 55% have clear abilities, 43% have sufficient abilities, and 2% have less abilities. Thus, the form of contribution to the science of guidance and counseling and education is expected to be implemented (reality therapy) together with stakeholders in order to achieve optimal and comprehensive success