A Clinical Study on Management of Incomplete Abortion by Manual Vacuum Aspiration (MVA)

Background: Abortion is an important social and public health issue. In Bangladesh complication from unsafe abortion is one of the leading causes of maternal mortality. It is a serious health problem. World Health Organisation estimates that 14% of maternal deaths which occur every year in the countries of South Asia including Bangladesh are due to abortion. Study shows manual vacuum aspiration procedure is safe and effective in incomplete abortion. Very few clinical trials were carried out in Bangladesh to assess the safety and effectivity of manual vacuum aspiration in managing incomplete abortion. Objective: To find out the outcome of manual vacuum aspiration in the management of patients of incomplete abortion. Materials and Methods: This observational descriptive study was conducted in the department of Obstetrics & Gynaecology, Dhaka Medical College & Hospital from June to December, 2004. One hundred cases of diagnosed incomplete abortion up to 12 weeks of gestation were managed by manual vacuum aspiration during this period. A data recording sheet was designed for this purpose. Haemodynamically stable patients with no history of induced abortion and fever were enrolled. Results: Procedure time of manual vacuum aspiration was short, average duration was 7 minutes. Bleeding was minimum (20-30 mL) in 67% cases and weighted mean was 29.80 mL. Eighty three percent patients were stable during the procedure and only 3% needed blood transfusion. Nonnarcotic analgesics were used in 59% cases and 33% needed only proper counselling. Average duration of hospital stay was 2 hours. Effectiveness of the procedure was about 98% with very low post procedure complication rate (2%). Conclusion: MVA procedure is a safe and effective technique of uterine evacuation in incomplete abortion. It is quick, less expensive, effective and less painful. Hospital stay and chance of perforation of uterus is less. So this procedure should be considered by health care system in Bangladesh for improving treatment of incomplete abortion to reduce both maternal morbidity and mortality

Quality evaluation and storage stability of mixed fruit leather prepared from mango, banana and papaya

This study aimed to elucidate the formulation and quality evaluation of fruit leather prepared from Mango, Banana and Papaya, and analyzes shelf life of developed samples at different storage conditions. Three samples of fruit leather (F1=50% mango pulp+20% banana pulp+19% papaya pulp, F2= 70% mango pulp+10% banana pulp+9% papaya pulp, F3=60% mango pulp+15% banana pulp+14% papaya pulp) were developed. The analysis of different composition such as moisture, ash, TSS (Total soluble solids), total sugar, acidity, crude fiber and ascorbic acid of all fresh fruit pulps and developed fruit leathers were taken place. The moisture, ash, and total sugar content of fruit leathers were in the range of 10.99 to 11.69%, 1.13-1.36%, and 54.08-55.38%, respectively. The fiber content ranged from 1.13 to 1.5% and sample F2 contained the highest amount (1.50%). The vitamin C content was highest in F2 (17.49 mg/ 100 g), while F3 gave the lowest (7mg/100g). The acidity of F3 was highest (0.21%), followed by F2 (0.16%) and F1 (0.12%). The sensory properties like color, texture, flavor and overall acceptability of mixed fruit leather of sample F2 (mango 70%, banana 10%, papaya 9%) was more acceptable than sample F1 and F3 which indicates mango rich leathers were much better than Papaya and banana rich leathers. The mixed fruit leathers were packed in sealed low-density and high-density polyethylene, and stored both at room temperature (25±1°C) and refrigerated temperature (4±1°C). Products were acceptable up to 4 months of storage and remained better in high-density polyethylene at room temperature than other conditions. [J Bangladesh Agril Univ 2022; 20(3.000): 323-332

An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity.

IntroductionThe liver, the most important metabolic organ of the body, performs a wide variety of vital functions. Hepatic cell injury occurs by the activation of reactive oxygen species (ROS) that are generated by carbon tetrachloride (CCl4), xenobiotics, and other toxic substances through cytochrome P450-dependent steps resulting from the covalent bond formation with lipoproteins and nucleic acids. Observing the urgent state of hepatotoxic patients worldwide, different medicinal plants and their properties can be explored to combat such free radical damage to the liver. In vivo and in silico studies were designed and conducted to evaluate the antioxidant and hepatoprotective properties of Gynura procumbens in rats.Materials and methodsGynura procumbens leaves were collected and extracted using 70% ethanol. The required chemicals CCl4, standard drug (silymarin), and blood serum analysis kits were stocked. The in vivo tests were performed in 140 healthy Wister albino rats of either sex under well-controlled parameters divided into 14 groups, strictly maintaining Institutional Animal Ethics Committee (IEAC) protocols. For the histopathology study, 10% buffered neutral formalin was used for organ preservation. Later the specimens were studied under a fluorescence microscope. In silico molecular docking and absorption, distribution, metabolism, excretion, and toxicity (ADMET) studies were performed, and the results were analyzed statistically.Results and discussionGynura procumbens partially negate the deleterious effect of carbon tetrachloride on normal weight gain in rats. The elevated level of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), creatinine, LDH, total cholesterol (TC), low-density lipoprotein (LDL), triglycerides (TG), malondialdehyde (MDA), deoxyribonucleic acid (DNA) fragmentation ranges, gamma-glutamyl transferase (γ-GT) in CCl4 treated groups were decreased by both standard drug silymarin and G. procumbens leaf extract. We have found significant & highly significant changes statistically for different doses, here p<0.05 & p<0.01, respectively. On the other hand, G. procumbens and silymarin displayed Statistically significant (p<0.05) and high significant(p<0.01) increased levels of HDL, CAT SOD (here p<0.05 & p<0.01 for different doses) when the treatment groups were compared with the disease control group. Because the therapeutic activity imparted by plants and drugs accelerates the movement of the disturbed pathophysiological state toward the healthy state. In the molecular docking analysis, G. procumbens phytoconstituents performed poorly against transforming growth factor-beta 1 (TGF-β1) compared to the control drug silymarin. In contrast, 26 phytoconstituents scored better than the control bezafibrate against peroxisome proliferator-activated receptor alpha (PPAR-α). The top scoring compounds for both macromolecules were observed to form stable complexes in the molecular dynamics simulations. Flavonoids and phenolic compounds performed better than other constituents in providing hepatoprotective activity. It can, thus, be inferred that the extract of G. procumbens showed good hepatoprotective properties in rats

Effect of various treatment specimens on the Triglyceride level of CCl4- treated rats.

Comparison of triglyceride (mg/dl) of rats, belonged to 14 groups just before sacrifice. Each group consist of 10 rodents each with equal body mass index. The data were expressed as mean±standard deviation. X-axis represents the group distribution and y-axis represents triglyceride level of different groups. All abbreviation of different groups has been mentioned in Table 1. (*indicates statistically significant change where p<0.05, correlation is significant at a 95% confidence interval and **indicates highly significant change where p<0.01, correlation is significant at a 99% confidence interval).</p

Interactions of compounds 44, 48, 50, and 51 with TGF-β type I receptor.

Interactions of compounds 44, 48, 50, and 51 with TGF-β type I receptor.</p

ADME properties of the phytoconstituents of interest.

ADME properties of the phytoconstituents of interest.</p

Interactions of the top 10 binding affinity phytoconstituents with PPAR-α.

Interactions of the top 10 binding affinity phytoconstituents with PPAR-α.</p

Effect of various treatment specimens on the total cholesterol level of CCl4- treated rats.

Comparison of total cholesterol (mg/dl) level of rats, belonged to 14 groups just before sacrifice. Each group consist of 10 rodents each with equal body mass index. The data were expressed as mean±standard deviation. X-axis represents the group distribution and y-axis represents total cholesterol level of different groups. All abbreviation of different groups has been mentioned in Table 1. (*indicates statistically significant change where p<0.05, correlation is significant at a 95% confidence interval and **indicates highly significant change where p<0.01, correlation is significant at a 99% confidence interval).</p

Effect of various treatment specimens on the HDL level of CCl4- treated rats.

Comparison of HDL level (mg/dL) of rats, belonged to 14 groups just before sacrifice. Each group consist of 10 rodents each with equal body mass index. The data were expressed as mean±standard deviation. X-axis represents the group distribution and y-axis represents HDL level of different groups. All abbreviation of different groups has been mentioned in Table 1. (*indicates statistically significant change where p<0.05, correlation is significant at a 95% confidence interval and **indicates highly significant change where p<0.01, correlation is significant at a 99% confidence interval).</p

RMSD, solvent accessible surface area, polar surface area, molecular surface area, and radius of gyration of the free and compound 8-bound form of PPAR-α.

RMSD, solvent accessible surface area, polar surface area, molecular surface area, and radius of gyration of the free and compound 8-bound form of PPAR-α.</p