THE INCREASE OF TOOTH ENAMEL SURFACE HARDNESS AFTER APPLICATION BLOOD COCKLE SHELLS (ANADARA GRANOSA) PASTE AS REMINERALIZATION AGENT

Objective: To determine the increase of tooth enamel surface hardness after application hydroxyapatite paste that was synthesized from blood cockle shells (Anadara granosa) as a remineralization agent. Methods: Laboratory experimental study using twenty-seven maxillary first premolar and randomly divided into 3 groups. All of the samples were immersed in the non-cola carbonated drink (2 min). Thereafter, samples in each group were treated (6 min) with application of blood cockle shells paste that has been synthesized (group 1), casein phosphopeptide-amorphous calcium phosphate paste (GC Tooth Mousse®) (group 2) as a positive control, and stored in saline solution (NaCl) (group 3) as a negative control. Vickers Hardness Number (VHN) measurement was performed at baseline, after immersing in non-cola carbonated drink and after completing of the respective treatment. Results: Immersion in non-cola carbonated drink reduced the enamel surface hardness significantly. Significant re-hardening after treated occurred in group 1 and 2 also baseline hardness of both groups were achieved. But statistically no significant differences between group 1 and 2 in re-hardening enamel surface hardness (final hardness-hardness after immersion). Conclusion: Application of blood cockle shells paste as a remineralization agent could increase tooth enamel surface hardness which is nearly the same effective as CPP-ACP paste

Viabilities of Odontoblast Cells Following Addition of Haruan Fish in Calcium Hydroxide

Background: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing and anti-inflammation. This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. Materials and Methods: Firstly, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by MTT assay on 24 h after the last treatment. While cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 µg/mL). Untreated cells were used as control. Result: Adding of HFE at range 25.0 till 100 µg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p<0.05). Conclusion: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent. BACKGROUND: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing, and anti-inflammation. AIM: This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. MATERIALS AND METHODS: First, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay on 24 h after the last treatment, while cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 μg/mL). Untreated cells were used as control. RESULTS: Adding of HFE at range 25.0 until 100 μg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p < 0.05). CONCLUSION: HFE effectively increased the viability of odontoblast MDPC-23 cells and has the potency to be used together to avoid the negative side effect of (CaOH)2 as a capping agent

The Potential of Trigona spp. Propolis as an Antioxidant Agent to Reduce Residual Peroxide after Intra-Coronal Bleaching Treatments

The present study aimed to determine the effectiveness of Trigona spp. propolis as an antioxidant to reduce residual peroxide after intra-coronal bleaching treatments. Thirty-five maxillary central incisors were divided into seven groups: five samples without antioxidants; sodium ascorbate 10% combined with Tween 80 0.2%; and Trigona spp. propolis 10%. The lengths of the application time were 1 h, 24 h, and 48 h. Each application time consisted of five samples. Root resection followed by artificial discoloration was performed in the samples. Then, intra-coronal bleaching using 35% hydrogen peroxide was applied. After the tooth color changed, the bleaching material was cleared, and this was followed by the applications of sodium ascorbate 10% combined with Tween 80 0.2% and Trigona spp. propolis 10%. The peroxide residue was measured by assessing dissolved oxygen using a titration analysis with either the Winkler or iodometric method. Data were analyzed using the ANOVA test and Tukey’s HSD test. The lowest peroxide residue amount was found with the application of antioxidants for 48 h after the intra-coronal bleaching treatment using 35% hydrogen peroxide. However, there was no significant difference between sodium ascorbate 10% combined with Tween 80 0.2% and Trigona spp. propolis 10% to reduce peroxide residues after the intra-coronal bleaching treatment (p > 0.05). Therefore, these findings indicate that Trigona spp. propolis 10% effectively reduces peroxide residues after intra-coronal bleaching treatments, which can interfere with the bond of the composite to the tooth surface and shorten the wait time for composite restorations after bleaching treatments

Treatment of internal resorption with MTA: A Case Report

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Internal Bleaching of discolored tooth with calcific metamorphosis abnormality in radiographic finding

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Penanganan Kedaruratan Endodontik Pada Pulpis Ireversibel

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APIKAL SEALING SEALER BERBAHAN DASAR EPOXY RESIN DAN SEALER BERBAHAN DASAR MINERAL TRIOXIDE AGGREGATE (MTA)

XIX,53

EFEKTIFITAS EKSTRAK DAUN JAMBU BIJI ( Psidium guajava) TERHADAP BAKTERI Enterococcus faecalis SEBAGAI SALA SATU BAHAN ALTERNATIF IRIGASI SALURAN AKAR

X,35

PERBANDINGAN BOND STRENGHT CORE BUILD UP PADA GIGI INSISIVUS DENGAN INTACT DENTIN DAN SKLEROTIK DENTIN

core build-up adalah restorasi yang ditempatkan pada struktur gigi yang rusak untuk mengembalikan sebagian besar bagian koronal gigi.prosedur klinis bahan adesif melibatkan dentin sklerotik atau karies yang melibatkan dentin sklerotik atau karies yang melibatkan dentin.xviii,77