Structural characterization of the DRF1 gene of Aegilops speltoides and comparison of its sequence with those of B and other Triticeae genomes

AbstractThe genus Aegilops L. has been intensively investigated due to its close relationship with wheat (Triticum L.) as contributor of B and D subgenomes. Because of their vast genetic diversity, Aegilops species represent a rich source of alleles of agronomic interest, which could be used to widen the wheat gene pool and improve tolerance to diseases, pests, drought, cold and other environmental stresses. We report the isolation and characterization of the Dehydration Responsive Factor 1 (DRF1) gene in three accessions of Ae. speltoides coming from different regions of the Fertile Crescent. The DRF1 gene belongs to the DREB gene family and encodes transcription factors which play a key role in plant response to water stress. As in other cereals, the DRF1 gene in Aegilops speltoides consists of four exons and three introns and undergoes alternative splicing. A processed pseudogene was also identified and compared with the sequence of an actual mRNA transcript, breaking new ground in the understanding of the complex regulation mechanism of this gene. The genetic diversity was evaluated by comparison of inter- and intra-species variation among some Aegilops and Triticeae, by considering both the whole gene and exon 4 sequences. The phylogenetic analyses were able to cluster the sequences in well-supported clades attributable to the genomes analysed. The overall results suggest that there is a high similarity between the B and S genome copies of the DRF1 gene but also features indicating that the two genomes have evolved independently

Triticale for Bioenergy Production

Abstract The promotion of renewable energy represents a target of the European 2020 strategy for growth. Plant biomass and organic wastes from agriculture represent an effective resource to be exploited for a sustainable rural development, optimizing the land use, diversifying rural entrepreneurship. Cereals are considered a promising biomass producing crop in temperate regions of Europe to be used for both fuel alcohol and biogas production. In particular, triticale shows a number of advantages such as high grain yield even in marginal environments, tolerance to drought, tolerance to more acid soils, low susceptibility to biotic stresses and is known to have reduced production costs. The characteristics of triticale were reviewed, focusing on bio-energy applications. Furthermore, data from a two-year experiment carried out in Italy using nine triticale lines grown in marginal areas close to Bracciano, Italy, were reported. A bread wheat variety selected for bio-energy application, EW9, were also included for a more complete analysis. Traits such as day-to-heading, plant height, number of plants, number of spikes, grain yields were analysed. Preliminary results concerning biogas potential of biomass consisting of triticale hay harvested at milky-dough phase were also measured and results are reported

Identifying Strategies for Energy Consumption Reduction and Energy Efficiency Improvement in Fruit and Vegetable Producing Cooperatives: A Case Study in the Frame of TESLA Project☆

Abstract TESLA (Transfering Energy Save Laid on Agroindustry) is a EU project pointing to the reduction of energy consumption and the improvement of energy efficiency in key agro-food sectors' cooperatives, as those processing fruit and vegetables. After a general analysis of energy consumptions during the first phase of the project, the processes responsible for the higher energy consumptions in these fruit and vegetable industries, as cold storage, have been identified. In the second phase of the project, a few case studies aimed at proposing customized solutions for reducing energy wastage and for improving energy efficiency in specific selected cooperatives have been performed. In this manuscript we report preliminary results of a case study carried out in an Italian horticulture cooperative having several production lines for fresh (1 st range) and minimally processed (4 th range) fruit and vegetable products. In this cooperative, an in-depth energy audit has been performed, and additionally a process simulation software has been applied to model, evaluate and improve the operations in this processing centre and in the supply chain from the primary production sites. Such case study may be used as an example for similar cooperatives of the fruit and vegetables sector, thus contributing in making this sector more economically and energetically sustainable

Soy-Based Infant Formula: Are Phyto-Oestrogens Still in Doubt?

Although Scientific Societies have stated that there are very few indications for the use of soy-based formula (SF) in infant nutrition, their utilization rates have been repeatedly found to be higher than expected. It is likely that a significant role in this regard is played by the belief that the use of SF during infancy can reduce the risk of the development of several diseases later in life. Although no definitive data that can substantiate these claims have been collected, many people perceive soy consumption to confer significant health benefits and might also use soy for infant nutrition. However, not all the problems regarding safety of SF in infants have been definitively solved. Among risks, the potentially toxic role of the phyto-oestrogens contained in SF is not definitively established. In vitro and animal studies have raised suspicions that SF could have potentially negative effects on sexual development and reproductive function, neurobehavioral development, immune function, and thyroid function. Several studies in humans have aimed to assess whether the results of animal studies can be applied to humans and whether SF can be used in infants following the official recommendations. The results are somewhat conflicting. The aim of this narrative review is to discuss what is presently known regarding the impact of phyto-oestrogens in SF on early and late child development. PubMed was used to search for the studies published from January 1980 to June 2017 using the keywords: “soy,” “soy formula,” “child,” “phytoestrogens.” Analysis of the literature showed that a global evaluation of the impact of modern SFs on human development seems to suggest that their use is not associated with relevant abnormalities. Only children with congenital hypothyroidism need adequate monitoring of thyroid function

A MULTIDISCIPLINARY APPROACH TO CHARACTERIZE TRITICALE ELITE LINES FOR INDUSTRIAL USES

Plant biomass and organic wastes from agriculture represent an effective resource to be exploited fora sustainable rural development, optimizing the land use, diversifying rural entrepreneurship, producing energyand new income.Among crops, triticale is considered particularly interesting, showing several advantages such as highgrain yield even in marginal environments, tolerance to drought, tolerance to more acid soils, lower productioncosts and lower susceptibility to biotic stresses.In the frame of a long collaboration with CIMMYT (International Maize and Wheat Improvement Center-MX), eight triticale elite lines from Mexico were grown in Italy using marginal lands. An Italian triticalevariety and a bread wheat line, specifically bred for bioenergy applications, were used for comparison. Overthree growing seasons, different agronomic evaluations were obtained harvesting at milk phase. In the thirdgrowing season, a set of parcels was harvested also at maturity, to evaluate grain yields and quality parametersfrom seeds.Milk phase biomass were properly dried and characterized following reference procedures, then used assubstrate for anaerobic digestion to produce biogas and methane in lab-scale experiments.Seeds from interesting lines were used to test different quality parameters including falling number inorder to evaluate the fermentation ability. RAPD technique by two different panels of primers was used togenotype a selection of lines

How future surgery will benefit from SARS-COV-2-related measures: a SPIGC survey conveying the perspective of Italian surgeons

COVID-19 negatively affected surgical activity, but the potential benefits resulting from adopted measures remain unclear. The aim of this study was to evaluate the change in surgical activity and potential benefit from COVID-19 measures in perspective of Italian surgeons on behalf of SPIGC. A nationwide online survey on surgical practice before, during, and after COVID-19 pandemic was conducted in March-April 2022 (NCT:05323851). Effects of COVID-19 hospital-related measures on surgical patients' management and personal professional development across surgical specialties were explored. Data on demographics, pre-operative/peri-operative/post-operative management, and professional development were collected. Outcomes were matched with the corresponding volume. Four hundred and seventy-three respondents were included in final analysis across 14 surgical specialties. Since SARS-CoV-2 pandemic, application of telematic consultations (4.1% vs. 21.6%; p < 0.0001) and diagnostic evaluations (16.4% vs. 42.2%; p < 0.0001) increased. Elective surgical activities significantly reduced and surgeons opted more frequently for conservative management with a possible indication for elective (26.3% vs. 35.7%; p < 0.0001) or urgent (20.4% vs. 38.5%; p < 0.0001) surgery. All new COVID-related measures are perceived to be maintained in the future. Surgeons' personal education online increased from 12.6% (pre-COVID) to 86.6% (post-COVID; p < 0.0001). Online educational activities are considered a beneficial effect from COVID pandemic (56.4%). COVID-19 had a great impact on surgical specialties, with significant reduction of operation volume. However, some forced changes turned out to be benefits. Isolation measures pushed the use of telemedicine and telemetric devices for outpatient practice and favored communication for educational purposes and surgeon-patient/family communication. From the Italian surgeons' perspective, COVID-related measures will continue to influence future surgical clinical practice

Echium vulgare and Echium plantagineum: A Comparative Study to Evaluate Their Inclusion in Mediterranean Urban Green Roofs

Green roofs (GRs) are proposed to offset against numerous environmental and socio-economic concerns associated with climate change and urban sprawl. In Mediterranean urban areas, to protect and conserve biodiversity through GRs, the use of native plant species from arid environments and with shallow roots is generally recommended. In north Europe, Echium vulgare L. is widely used on GRs for its tolerance to abiotic stresses and its attractiveness for bees; unfortunately, since this species requires cold winters to induce flowering and warm wet summers for vegetative growths, its adaptability to Mediterranean GRs has been questioned. The current study is based on the hypothesis that Echium plantagineum L. can adapt better to the Mediterranean environment than E. vulgare and offer blooms to pollinators, thus providing the important urban ecosystem service (UES) of protecting entomofauna biodiversity. To compare the adaptability of E. plantagineum vs. E. vulgare, both Echium species were grown and studied on the extensive GR installed at ENEA Casaccia Research Center, in the north of Rome, Lazio, Italy. The comparative analysis of the GR performance of the two species was based on several plant-related traits, including seed morphology, rosette stage, inflorescence, flower and root-related traits, and their biological life cycle, most of them showing significant differences (for example, rosette area was 1.42-fold major in E. plantagineum than in E. vulgare). The information provided in this manuscript will be useful to update the herbarium records for conservation biology. A dramatic water stress was purposely applied in the GR before the end of the hot summer season, and while E. plantagineum faced with success the imposed dehydration (88.4% vegetation cover), E. vulgare did not (7.5% vegetation cover), presumably because of its biennial life cycle which did not allow it to complete seed maturation (only 46.9% percentage mature seeds in E. vulgare respect to 89.5% in E. plantagineum). In summary, as the main result, this work shows that in Mediterranean areas, the inclusion of E. plantagineum in seed mixes for flower meadow GRs could represent a valuable alternative to E. vulgare in temperate areas, providing a safeguard for pollinators and allowing water and energy saving