Optimatization in Bioconversion of Quercetin Glucosides Using Aspergillus Acueletus LS04-3

Microbial transformation is known to be useful for obtaining more active or less toxic compounds and achieving selective compounds conversion to more useful derivatives. The objective of this study is to explore several factors that affect the optimization of bioconversion of quercetin glucosides using Aspergillus acueletus LS04-3. To obtain the optimum condition of biotransformation the fungus was cultured under several conditions by varying the number of days of fermentation, the concentration of substrate, carbon, and nitrogen source. The transformation product was analyzed using HPLC and LCMS-MS. The results revealed that on the third-day biotransformation process, quercetin production reached the highest amount and the optimum concentration was at 50 ppm of quercetin glucoside based on HPLC analysis. In addition, from various carbon sources, glucose yielded the highest biotransformation product, while nitrogen accelerated the reaction. In this research, media of A. acueletus LS04-3 containing carbon and nitrogen could increased quercetin production. This research finding provided several factors for optimizing quercetin biotransformation by A. acueletus LS04-3. These factors were important in scaling up biotransformation even in other biotransformation reactions

The Evaluation of Substrates and Trichoderma SP. Isolates for Cellulase Production

As higher interest was on the lignocellulose-based or second generation bioethanol production, the research was then more focused on the production of cellulase, especially on the domestic enzyme. Trichoderma sp. is considered as one of the most efficient producer of cellulase. This study was conducted to investigate the performance of Trichoderma sp. on a variety of substrates to produce cellulase. Three types of substrate variations and three types of Trichoderma sp. were used in this experiment. The substrate used were wheat bran, rice bran and oil palm empty fruit bunches (EFBs), whereas Trichoderma sp. isolates were encoded as T004, T051 and T063. Production of cellulase was made by solid fermentation for 7 days. The analysis of cellulase activity was done by National Renewable Energy Laboratory (NREL) method for filter paper assay. The results showed that the type of substrate affected the performance of Trichoderma sp. All types of fungus produced cellulase on wheat bran substrate with activity of 0.52 FPU /ml for T004, 0.23 FPU/ml for T051 and 0.27 FPU /ml for T063. With the rice bran substrate and EFBs, only T004 could produce cellulase and the enzyme activity analyzed were 0.08 FPU /ml and 0.008 FPU/ml respectively. Optimation of the buffer addition on enzyme extraction process produces the highest activity 0.85 FPU/mL for T004 with wheat bran substrate.

Metode Hplc-dad Untuk Analisis Glukosamin Pada Teripang Kering Dengan Derivatisasi Prekolom (Hplc-dad Method for Glucosamine Analysis in Dried Sea Cucumber with Precolumn Derivatization)

Glukosamin dikenal sebagai suplemen untuk mengobati osteoartritis dan nyeri sendi. Baru-baru ini masyarakat pesisir Indonesia telah mengembangkan teripang yang diperkaya glukosamin sebagai alternatif pengganti krustasea yang merupakan sumber glukosamin yang banyak dikonsumsi. Tujuan dari penelitian ini adalah melakukan pengembangan dan validasi metode high performance liquid chromatography dengan diode array detector (HPLC-DAD) untuk penentuan glukosamin pada produk teripang. Glukosamin diekstraksi dari sampel teripang kering melalui hidrolisis menggunakan asam klorida 5 M, dilanjutkan dengan derivatisasi menggunakan larutan Fmoc-Cl (9-Fluorenylmethyl chloroformate) dan buffer borat sebelum dilakukan analisis menggunakan HPLC-DAD. Semua langkah preparasi sampel dan standar dilakukan dengan menggunakan metode pengenceran gravimetri. Pemisahan kromatografi Fase terbalik dilakukan di kolom C18 dengan menerapkan elusi gradien menggunakan air dan asetonitril sebagai Fasa gerak. Hasil penelitian menunjukkan linieritas yang baik dengan koefisien determinasi sebesar 0,999 pada rentang konsentrasi 1 sampai 220 mg/kg. Metode ini memiliki LOD dan LOQ masing-masing 0,16 dan 0,53 mg/kg, sedangkan nilai perolehan kembali berada di kisaran 98-99%. Nilai presisi intraday ditemukan lebih rendah dari 2% untuk empat konsentrasi spiking yang berbeda (10, 60, 100 dan 200 mg/kg). Metode ini kemudian berhasil diterapkan untuk menganalisis glukosamin pada dua puluh sampel teripang kering lokal. Hasilnya menunjukkan bahwa sebelas sampel mengandung glukosamin dalam rentang 100 hingga 2700 mg/kg

Proceedings of the 3rd International Conference on Community Engagement and Education for Sustainable Development

This proceeding contains articles on the various ideas of the academic community presented at The 3rd International Conference on Community Engagement and Education for Sustainable Development (ICCEESD 2022) organized by the Universitas Gadjah Mada, Indonesia on 7th-8th December 2022.  ICCEESD is a biannual forum for sharing, benchmarking, and discussing HEI’s activities in developing Education for Sustainable Development towards community engagement. Education for Sustainability as a teaching strategy for resolving community challenges through formal, informal, or non-formal education is expected to benefit from various community service best practices by academics, researchers, and students. The 3rd ICCEESD has “Strengthening Education for Sustainability Towards Better Community Engagement” as its theme this year. It is expected that the 3rd ICCEESD will provide a forum for the presenters and participants to exchange best practices, policies, and conceptual implementation of Education for Sustainability towards better community engagement and explore ideas to address community needs.  Conference Title: 3rd International Conference on Community Engagement and Education for Sustainable DevelopmentConference Theme: Strengthening Education for Sustainability Towards Better Community EngagementConference Acronyms: ICCEESD 2022Conference Date: 7th-8th December 2022Conference Location: Grand Rohan Jogja Yogyakarta, IndonesiaConference Organizer: Universitas Gadjah Mada, Indonesi