Derivatisasi Morfin Menggunakan Dansil Klorida untuk Meningkatkan Kepekaan Deteksi Morfin pada Metode Klt- Spektrofotodensitometer

Fluorescence detection could be used for enhance the sensitivity of morphine detection by TLC-Spectrophotodensytometry. Morfin is a compound that fluoresces intrinsically incapable. ence, morphine was derivatized using dansyl chloride. Morphine was derivatized by two methods, derivatization in solution and overspotting method. The derivatization reaction was kept at pH 9 and 450 C for 20 minute. The ratio between dansyl chloride and analyte concentration was 6:1. The derivatization result was separated from the interference using TLC. The TLC method was using Al-TLC silica G60 plate as a stationary phase and etyl acetate : methanol : amonia (85:10:5) as the mobile phase. The dansyl derivative was detected at ?eks 365 nm and K540 filter for fluoresence. Derivatization of morphine-dansyl chloride capable to enhancing the sensitivity of morphine detection using TLC-Spectrophotodensytometry. Derivatization in solution and overspotting method took the same time in procedure but they gave different sensitivity. Derivatization in solution method gave more quantity than derivatization of overspotting