Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application

<p>Abstract</p> <p>Background</p> <p>Nanoparticle-metal oxide and gold represents a new class of important materials that are increasingly being developed for use in research and health related activities. The biological system being extremely critical requires the fundamental understanding on the influence of inorganic nanoparticles on cellular growth and functions. Our study was aimed to find out the effect of iron oxide (Fe₃O₄), gold (Au) nanoparticles on cellular growth of <it>Escherichia coli </it>(<it>E. coli</it>) and also try to channelize the obtained result by functionalizing the Au nanoparticle for further biological applications.</p> <p>Result</p> <p>Fe₃O₄and Au nanoparticles were prepared and characterized using Transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS). Preliminary growth analysis data suggest that the nanoparticles of iron oxide have an inhibitory effect on E. coli in a concentration dependant manner, whereas the gold nanoparticle directly showed no such activity. However the phase contrast microscopic study clearly demonstrated that the effect of both Fe₃O₄and Au nanoparticle extended up to the level of cell division which was evident as the abrupt increase in bacterial cell length. The incorporation of gold nanoparticle by bacterial cell was also observed during microscopic analysis based on which glutathione functionalized gold nanoparticle was prepared and used as a vector for plasmid DNA transport within bacterial cell.</p> <p>Conclusion</p> <p>Altogether the study suggests that there is metal nanoparticle-bacteria interaction at the cellular level that can be utilized for beneficial biological application but significantly it also posses potential to produce ecotoxicity, challenging the ecofriendly nature of nanoparticles.</p

Burkitt's Lymphoma as Primary Adrenal Lymphoma Diagnosed on Fine Needle Aspiration Cytology : A Rare Case Report

Introduction :Primary adrenal lymphoma presenting as Burkitt’s lymphoma  is an extremely rare entity. Although biopsy or excision is often needed for definitive diagnosis computed tomography guided fine needle aspiration has great diagnostic value in detection of primary adrenal lymphoma.Presentation of case : A case  of a 20 yr old male with a right adrenal mass diagnosed as Burkitts lymphoma on Fine Needle Aspiration Cytology (confirmed by bone marrow and cytogenetics) has been reported.Conclusion : Fine Needle Aspiration Cytology  as an initial investigation tool can be very effective for detection of unusual presentation like an adrenal mass in a case of Burkitt's lymphom

Secondary Signet Ring Cell Carcinoma of Prostate

True metastases to prostate from solid tumors are reported only in 0.2% of all surgical prostatic specimens and 2.9% of all male postmortems. Clinical context, morphological features, and immunohistochemical localization of prostate specific antigen (PSA) are supposed to clarify the differential diagnosis between a secondary and a primary tumor. We report an unusual and rare case of secondary signet ring cell carcinoma (SRCC) of prostate in which the clinical data and signet ring cell morphology pointed toward the diagnosis of a primary SRCC. Immunohistochemistry (IHC) for PSA not only proved the case to be a secondary SRCC but also initiated the process for diagnosis of the occult primary malignancy in the patient&#8217;s stomach.Keywords: Prostate specific antigen, prostate, signet ring cell carcinomaNigerian Journal of Surgery, Jan-Jun 2012 | Volume 18 | Issue

A study of the waves and boundary layers due to a surface pressure on a uniform stream of a slightly viscous liquid of finite depth

The 2D problem of linear waves generated by an arbitrary pressure distribution p0(x,t) on a uniform viscous stream of finite depth h is examined. The surface displacement ζ is expressed correct to O(ν) terms, for small viscosity ν, with a restriction on p0(x,t). For p0(x,t)=p0(x)eiωt, exact forms of the steady-state propagating waves are next obtained for all x and not merely for x≫0 which form a wave-quartet or a wave-duo amid local disturbances. The long-distance asymptotic forms are then shown to be uniformly valid for large h. For numerical and other purposes, a result essentially due to Cayley is used successfully to express these asymptotic forms in a series of powers of powers of ν1/2 or ν1/4 with coefficients expressed directly in terms of nonviscous wave frequencies and amplitudes. An approximate thickness of surface boundary layer is obtained and a numerical study is undertaken to bring out the salient features of the exact and asymptotic wave motion in question

Juvenile xanthogranuloma-diagnostic challenge on fine-needle aspiration cytology

Juvenile xanthogranuloma (JXG) is a rare cutaneous lesion with paucity of literature on its cytological features. We report one such case which on fine-needle aspiration cytology yielded a mixed population of foamy histiocytes, multinucleated giant cells and variable admixture of lymphocytes and eosinophils causing diagnostic dilemma with other differentials, especially Langerhans cell histiocytosis. However, clinical correlation followed by histopathology confirmed the diagnosis. Hence, JXG has characteristic and diagnostic cytologic features

Role of fine needle aspiration cytology in primary tubercular epididymo-orchitis

Tubercular epididymo-orchitis in not uncommon in a highly tuberculosis-prevalent developing country like India. However, it closely mimics a testicular tumor clinically, radiologically, and even serologically. This may lead to an unnecessary orchidectomy. Nevertheless, fine needle aspiration cytology can provide adequate material for cytological and microbiological examination and it helps to avoid unnecessary orchidectomy when there is suspicion of a testicular tumor