ISOLASI DAN KARAKTERISASI BAKTERI PROTEOLITIK DARI LIMBAH CAIR INDUSTRI TAHU

Abstrak Penelitian ini bertujuan untuk mengisolasi bakteri proteolitik dari limbah cair industri tahu, mengetahui karakter dan indeks protease bakteri proteolitik terpilih dari limbah cair industri tahu. Produksi enzim protease oleh bakteri proteolitik diketahui dengan adanya zona bening di sekitar koloni bakteri pada media Skim Milk Agar (SMA). Perhitungan indeks proteolitik dilakukan pada bakteri yang memiliki aktivitas protease. Hasil penelitian menunjukkan terdapat 13 isolat yang memiliki aktivitas protease. Satu isolat (LTN 19) dengan indeks protease tertinggi sebesar 3,15 dipilih untuk dikarakterisasi morfologi, mikroskopik, dan biokimia. Berdasarkan karakterisasi yang telah dilakukan, isolat LTN 19 memiliki kemiripan dengan genus Staphylococcus dengan persentase kemiripan sebesar 85,7%. Kata kunci: bakteri proteolitik, enzim protease, limbah cair industri tahu Abstract The aims of this study were to do proteolytic bacteria isolation, to determine the character and protease index of selected proteolytic bacteria from tofu industry liquid waste. The production of protease enzyme by proteolytic bacteria is known by the presence of clear zone around bacterial colonies on Skim Milk Agar (SMA) media. Proteolytic index calculations are performed on bacteria that have protease activity. The results showed that there were 13 isolates that had protease activity. One isolate (LTN 19) with the highest protease index of 3.15 was selected for morphological, microscopic, and biochemical characterization. Based on the characterization that has been done, isolate LTN 19 has similarities with the genus Staphylococcus with a similarity percentage of 85.7%. Keywords: proteolytic bacteria, protease enzyme, tofu industry liquid wast