Profile and Evaluation of Dermatophytosis

Background: Dermatophytosis is an infection of the tissues, for example, the stratum corneum of the epidermis, hair, and nails, which is caused by dermatophyte fungi group. This retrospective study has never been done before and the 2011-2013 election was conducted due to changes in the health insurance system at RSUD Dr. Soetomo. Purpose: To evaluated patient management of dermatophytosis in Mycology Division Outpatient Clinic Dr. Soetomo General Hospital 2011-2013. Methods: A descriptive retrospective study the aim to evaluated general description, epidemiology, clinical manifestation, establisment diagnosis, management and repeated control new patient dermatophytosis. Results: Percentage new case  dermatophytes infection increased, but the number of visits to URJ for 3 years decreased. The most diagnosis is  tinea corporis. Most cases come from Surabaya. The most patient complaints are itching. The most efflorescence is polycyclic. Griseofulvin is the most therapeutic choice given in the case of tinea corporis. Conclusions: In this study most cases of dermatophytosis is tinea korporis. The diagnosis approach of dermatophytosis diagnosis was obtained by anamnesis, physical examination, and additional examination. Most of dermatophytosis  treated with antifungal griseofulvin

Artikel asli: Profil Kadar CXCL 10 Serum pada Pasien Vitiligo Di Unit Rawat Jalan Kesehatan Kulit Dan Kelamin RSUD Dr Soetomo Surabaya

Latar Belakang: Vitiligo adalah penyakit depigmentasi yang paling sering dijumpai dengan manifestasi klinis berupa makula berwarna putih susu berbatas tegas, patogenesis kompleks yang belum dipahami dengan baik sehingga evolusi penyakit tidak dapat diprediksi. Peran kemokin CXCL 10 pada vitiligo masih belum banyak diketahui dan dipelajari. Penelitian mengenai kadar CXCL 10 serum pada vitiligo belum pernah dilakukan di Indonesia. Tujuan: Untuk mengetahui profil kadar CXCL 10 serum pada pasien vitiligo. Metode: Rancangan penelitian ini deskriptif cross-sectional yang bertujuan untuk mengevaluasi kadar CXCL 10 serum pada pasien vitiligo, 16 sampel yang didiagnosis vitiligo dilakukan pengambilan darah pada pasien untuk mengukur kadar serum CXCL 10. Hasil: Kadar CXCL 10 serum pada penelitian ini lebih tinggi pada pasien vitiligo, tidak ada perbedaan bermakna antara laki laki dan perempuan dan distribusi proporsi tipe vitiligo tertinggi adalah generalisata. Simpulan: Profil kadar serum CXCL 10 pada pasien vitiligo berdasarkan jenis kelamin, usia, dan lama menderita vitiligo

The Role of Stem Cell Metabolites Derived From Placenta for Skin Regeneration An In Vitro Study

Background: The role of stem cells in skin aging is to repair injured tissue or replace other cells in programmed cell death. Stem cell metabolites are rich in growth factors including IL-10, IL-4, EGF, GM-CSF, and TGF-β that can induce the skin production of protein and elastic fbers, leading to the improvement of skin appearance. Aim: This study aimed to assess the characteristics of stem cell metabolites in vitro. Methods: Cytotoxicity assay was performed using MTT reagents and optical cell densities were determined using ELISA reader to fnd the percentage of living cells. Cytokine detection assay was performed by analyzing the cytokine levels in the peripheral blood mononuclear cell (PBMC) and mesenchymal stem cell (MSC) using ELISA. Apoptosis assay was performed using the double staining method with the markers identifed were Hsp70, p53, and caspase-3. Results: All samples showed the percentage of living cells that exceed 70%. Cytokine detection assay showed a decrease of IL- 12 and IFN-γ in both PBMC and MSC groups. The apoptosis assay of human adipose mesenchymal stem cells using a fluorescence microscope showed most of the green light was lost in control cells without metabolites. We found that the expressions of Hsp70 were increased while the expression of p53 and caspase-3 were decreased in the stem cell metabolites samples. Conclusion: These results showed that stem cell metabolites are non-toxic, do not cause a systemic immune response to surrounding tissue, and able to inhibit the occurrence of apoptosis

Pre-Clinical Trial Stem Cell Metabolites Derived From Placenta For Wound Healing

Previous research focuses on in vitro study of stem cell metabolites derived from placenta for wound healing. This study, however, is an advanced stage which focuses on testing the efficiency and efficacy of stem cell metabolites in rats (Rattus novergicus). The tests carried out examined the blood levels with ELISA instruments and integument histology by observing the activity of polymorph nuclear and monocyte cells in the control and treatment groups. In the control group, the rats were injured in the anterior and posterior back skin with a 1×1 cm incision wound, (only antibiotics), while the treatment group uses antibiotics and 4 mL injections of stem cell metabolites. Each group was repeated three times with the samples observed for blood levels using ELISA Interleukin-4, Interleukin-10 and Tumor Necrosis Factor-α, with integument histology at pre-injection, in days 1, 3 and 6. These were used to compare the development of inflammatory cells, polymorphonuclear and monocytes between the control and treatment groups. Stem cell metabolites are significantly effective and efficient with the ability to inhibit the inflammatory process in tissues in terms of examining the blood levels of rats using ELISA Interleukin-4, Interleukin-10 and Tumor Necrosis Factor-α. Interleukin-4 and Interleukin-10 (anti-inflammatory) tend to significantly increase the treatment group, while Tumor Necrosis Factor-α (pro inflammation) increases the control group. Histology results showed a decrease in the activity of polymorphonuclear and monocytes inflammatory cells in the treatment group compared to the control, which indicated that the stem cell metabolites were able to significantly inhibit the inflammatory process. It is concluded that stem cell metabolites derived from placenta are effective and efficient for wound healing in rats. Clinical study is needed for further research for it to be used on humans

Clinical Outcomes of Stem Cell Metabolites Formula Derived from Placenta for Skin Regeneration

Background: Previous research focused on in vitro and animal study of stem cell metabolites formula derived from the placenta for skin regeneration. Objective: This further study is a clinical trial to evaluate the effectiveness of stem cell metabolites formula in skin regeneration. Methods: Priorly, subjects’ facial conditions were examined and recorded using Janus skin analyzer to observe marks and blemishes present on their skin. Stem cell metabolites were applied on the face twice a day, day and night, with singleuse doses of three pumps. We evaluated before and after stem cell metabolites application using Janus over the period six months, with three times repeated evaluation every two months. The Janus evaluation results are Spot, Pore, Roughness, Wrinkle, UV Acne, UV Spot, and UV Moisture. Results: The percentage of spot, pore, roughness, wrinkle, UV acne, and UV spots were noticeably reduced. Meanwhile, the percentage of UV moisture increased in almost all subjects who were evaluated for six months. From a qualitative aspect, 90% of subjects are satisfied with the results of the application of stem cell metabolites. The subjects felt that facial wrinkles had reduced, the face was smoother and supple, and reduction in dark pigmentation. Conclusion: It can be concluded that stem cell metabolites are useful in face skin regeneration

Clinical Outcomes of Stem Cell Metabolites Formula Derived from Placenta for Skin Regeneration

Background: Previous research focused on in vitro and animal study of stem cell metabolites formula derived from the placenta for skin regeneration. Objective: This further study is a clinical trial to evaluate the effectiveness of stem cell metabolites formula in skin regeneration. Methods: Priorly, subjects’ facial conditions were examined and recorded using Janus skin analyzer to observe marks and blemishes present on their skin. Stem cell metabolites were applied on the face twice a day, day and night, with single-use doses of three pumps. We evaluated before and after stem cell metabolites application using Janus over the period six months, with three times repeated evaluation every two months. The Janus evaluation results are Spot, Pore, Roughness, Wrinkle, UV Acne, UV Spot, and UV Moisture. Results: The percentage of spot, pore, roughness, wrinkle, UV acne, and UV spots were noticeably reduced. Meanwhile, the percentage of UV moisture increased in almost all subjects who were evaluated for six months. From a qualitative aspect, 90% of subjects are satisfied with the results of the application of stem cell metabolites. The subjects felt that facial wrinkles had reduced, the face was smoother and supple, and reduction in dark pigmentation. Conclusion: It can be concluded that stem cell metabolites are useful in face skin regeneration

Correlation of Vitiligo Area Scoring Index with the amount of CXCL 10 serum in vitiligo patient

Introduction Vitiligo is a skin pigmentation disorder, often progressive and familial, characterized by depigmented macules on asymptomatic skin. This disorder is an acquired idiopathic depigmentation disorder, characterized by scaly/ non-scaly white macules resulting from the loss of skin melanocytes. CXCL 10 is one of the 3 CXCR 3 receptor ligands which have chemotaxic effects on various immune system cells. Serum CXCL 10 can be a new biomarker in monitoring disease activity. Methods This is a descriptive cross-sectional study aimed at evaluating serum CXCL 10 levels in vitiligo patients conducted at Dr. Soetomo general hospital Surabaya, 16 blood samples of patients diagnosed with vitiligo were taken to patients to measure serum CXCL 10 levels. Results Serum CXCL 10 levels in this study were higher in patients with stable vitiligo lesion activity and had high VASI values statistically, p = 0.000 (p = < 0.05). Conclusion There is positive correlation between VASI score and CXCL 10 serum levels

The role of stem cell metabolites derived from placenta for skin regeneration: An in vitro study

Background: The role of stem cells in skin aging is to repair injured tissue or replace other cells in programmed cell death. Stem cell metabolites are rich in growth factors including IL-10, IL-4, EGF, GM-CSF, and TGF-β that can induce the skin production of protein and elastic fibers, leading to the improvement of skin appearance. Aim: This study aimed to assess the characteristics of stem cell metabolites in vitro. Methods: Cytotoxicity assay was performed using MTT reagents and optical cell densities were determined using ELISA reader to find the percentage of living cells. Cytokine detection assay was performed by analyzing the cytokine levels in the peripheral blood mononuclear cell (PBMC) and mesenchymal stem cell (MSC) using ELISA. Apoptosis assay was performed using the double staining method with the markers identified were Hsp70, p53, and caspase-3. Results: All samples showed the percentage of living cells that exceed 70%. Cytokine detection assay showed a decrease of IL-12 and IFN-γ in both PBMC and MSC groups. The apoptosis assay of human adipose mesenchymal stem cells using a fluorescence microscope showed most of the green light was lost in control cells without metabolites. We found that the expressions of Hsp70 were increased while the expression of p53 and caspase-3 were decreased in the stem cell metabolites samples. Conclusion: These results showed that stem cell metabolites are non-toxic, do not cause a systemic immune response to surrounding tissue, and able to inhibit the occurrence of apoptosis

Clinical Outcomes of Stem Cell Metabolites Formula Derived from Placenta for Skin Regeneration

Background: Previous research focused on in vitro and animal study of stem cell metabolites formula derived from the placenta for skin regeneration. Objective: This further study is a clinical trial to evaluate the effectiveness of stem cell metabolites formula in skin regeneration. Methods: Priorly, subjects’ facial conditions were examined and recorded using Janus skin analyzer to observe marks and blemishes present on their skin. Stem cell metabolites were applied on the face twice a day, day and night, with single-use doses of three pumps. We evaluated before and after stem cell metabolites application using Janus over the period six months, with three times repeated evaluation every two months. The Janus evaluation results are Spot, Pore, Roughness, Wrinkle, UV Acne, UV Spot, and UV Moisture. Results: The percentage of spot, pore, roughness, wrinkle, UV acne, and UV spots were noticeably reduced. Meanwhile, the percentage of UV moisture increased in almost all subjects who were evaluated for six months. From a qualitative aspect, 90% of subjects are satisfied with the results of the application of stem cell metabolites. The subjects felt that facial wrinkles had reduced, the face was smoother and supple, and reduction in dark pigmentation. Conclusion: It can be concluded that stem cell metabolites are useful in face skin regeneration