4 research outputs found
Effect of DNase on the adhesion of <i>S. xylosus</i> to glass surface in flow cell.
<p>Black bars indicate untreated cells. Crossed bars indicate cells treated with DNase (50 µg/ml), washed and resuspended in PBS. White bars indicate cells resuspended in PBS containing DNase (50 µg/ml). Asterisk indicates statistically significant differences between samples with and without eDNA (t-test, *p<0.05, **p<0.01).</p
Effect of ionic strength and surface chemistries on eDNA mediated adhesion of <i>S. xylosus</i>.
<p>Black bars indicate untreated cells. White bars indicate cells treated with DNase. Experiments were carried out at low (I = 0.015 M), medium (I = 0.19 M) and high (I = 0.70 M) ionic strength. Values are average of 3 replicates (error bars  =  S.D.) Asterisk indicates statistically significant differences between samples with and without eDNA (t-test, *p<0.05, **p<0.01).</p
Cell surface properties with and without eDNA.
<p>Water contact angle and zeta potential measurements of <i>S. xylosus</i> cells with and without eDNA.</p><p>*indicates statistically significant difference (t-test, p<0.05).</p
Cilia-Mimetic Hairy Surfaces Based on End-Immobilized Nanocellulose Colloidal Rods
We show a simple
method toward nanoscale cilia-like structures,
i.e., functional hairy surfaces, upon topochemically functionalizing
nanorods of cellulose nanocrystals (CNCs) with thiol end groups (CNC-SHs),
which leads to their immobilization onto a gold surface from one end,
still allowing their orientational mobility. CNCs having a lateral
dimension of 3–5 nm and length of 50–500 nm incorporate
the native crystalline structure with hydrogen-bonded cellulose chains
in the parallel configuration. This facilitates asymmetric, selective
chemical modification of the reducing ends through reductive amination.
Successful thiol functionalization is demonstrated using cryo transmission
electron microscopy based on selective attachment of silver nanoparticles
to the CNC-SH ends to form Janus-like colloidal rod–sphere
adducts. The extent of thiol modification of CNC-SHs is quantified
using X-ray photoelectron spectroscopy. The promoted binding of CNC-SHs
on gold surfaces is shown by atomic force microscopy and quartz crystal
microbalance, where the high dissipation suggests pronounced orientational
mobility due to flexible joints at one rod end onto the surfaces.
That the joints are flexible is also shown by the bending and realignment
of the CNC-SH rods using a receding triple-phase evaporation front
of a drying drop of water. The ability of the hairy surface to size-selectively
resist particle binding was also investigated. As the CNCs are piezoelectric
and allow magnetic functionalization by nanoparticles, we foresee
a general platform for nanosized artificial cilia for fluid manipulation
and controlled adsorption/desorption