IMPACT OF WII-FIT TRAINING ON NEURO-MUSCULAR CONTROL

INTRODUCTION: In the past year, the interactive exercise video game Wii Fit (Nintendo, Tokyo, Japan) has achieved worldwide popularity. This system could be a potential asset for both training and physical therapy purposes; however, there is a lack of scientific validation to justify such applications. As a first step in ascertaining the advantages of the Wii Fit system, the present study is focused on the neuromuscular control changes that occur after 8 weeks of daily training. METHOD: Two healthy subjects (25.5±2.1 years, 177.8±14.37 cm, 71.5±16.26 kg) trained for 30 minutes a day for 8 consecutive weeks using standard Wii Fit strength training, aerobic, and yoga exercises. Before and after the training period, a series of tests were performed (gait, hop, isometric, and one leg stability) while collecting EMG data from the quadriceps (rectus femoris, vastus lateralis, vastus medialis), the hamstrings (biceps femoris and semitendinosus), and the grastrocnemii (lateralis and medialis). The EMG data was linear-enveloped and normalized by a maximum isometric voluntary contraction (MVIC). Similarly to Lloyd et al. (2005), the electromyographic activations were then summed by muscle group to calculate the co-contraction ratio (CCR), which is a value between 0 and 1 that indicates equalizing activation as it increases. RESULTS: Only the right leg data is being reported in this paper. Table 1 displays the CCR for the antagonist coactivations of the hamstrings and quadriceps and also the synergistic activations of the knee flexor muscle groups. It is worth noting that during gait and hopping motions, the ratios are decreased after training, while during the stability tests they increased. Finally, no trend emerged for the isometric data. Table 1 Co-contraction Ratio Maximums (Ext 60 and Flex 60 refer to isometric extesion and flexion at 60°)– values in italics are reciprocals Ext 60 Flex 60 Gait Hop Stability Ham/Quad Coactivation Pre 0.21 0.41 0.17 0.20 0.06 Post 0.46 0.47 0.02 0.06 0.88 Ham/Gast Synergy Pre 0.34 0.47 0.02 0.08 0.04 Post 0.47 0.28 0.01 0.08 0.35 DISCUSSION: After training, the CCR data for dynamic activity indicated more focused muscle control. During the stability tests, much higher CCR values were reported, indicating the muscles were doing a better job achieving a intra-articular equilibrium. CONCLUSION: These preliminary results indicate a promising use of the Wii Fit system for training and physical therapy as on a small population they demonstrated neuromuscular control improvement during dynamic and static trials. REFERENCES: Lloyd, D. G., Buchanan, T. S., and Besier, T. F. (2005). Neuromuscular Biomechanical Modeling to Understand Knee Ligament Loading. Medicine & Science in Sports & Exercise, 37, 1939-1947

Histological analysis of the expression of Agrobacterium rhizogenes rolB-GUS gene fusions in transgenic tobacco

The gene rolB from the T-DNA of Agrobacterium rhizogenes is capable of directing differentiation of roots in transformed plant cells. A detailed histological analysis of the expression of this gene in transgenic tobacco plants is presented here. Gene fusions between the promoter of rolB and the GUS reporter gene (encoding E. coli beta-glucuronidase) were utilized: tissue and cell specific GUS activity as driven by a long (1185 base pairs) and a short (306 bp) version of the rolB promoter was visualized histochemically during different phases of plant development, from embryo to flowering. The extended (1185 bp) rolB promoter is under developmental control and is specifically active in the initial cells of all types of meristems and in the vascular system (phloem, phloem parenchyma, xylem parenchyma, pericycle and pericycle-like cells) of mature organs as well as in the central cylinder of the embryo. In contrast, the 306 bp deletion of the promoter is unable to drive expression in meristematic initials but is still active in the vascular system of the seedling