Colonic Protein Fermentation and Promotion of Colon Carcinogenesis by Thermolyzed Casein

Thermolyzed casein is known to promote the growth of aberrant crypt foci (ACF) and colon cancer when it is fed to rats that have been initiated with azoxymethane. We speculated that the promotion was a consequence of increased colonic protein fermentation (i.e., that the thermolysis of the casein decreases its digestibility, increases the amount of protein reaching the colon, and increases colonic protein fermentation and that the potentially toxic products of this fermentation promote colon carcinogenesis). We found that the thermolysis of casein reduces its digestibility and increases colonic protein fermentation, as assessed by fecal ammonium and urinary phenol, cresol, and indol-3-ol. Thermolysis of two other proteins, soy and egg white protein, also increases colonic protein fermentation with increased fecal ammonia and urinary phenols, and thermolysis of all three proteins increases the levels of ammonia and butyric, valeric, and i-valeric acids in the cecal contents. We found, however, that the increased protein fermentation observed with thermolysis is not associated with pro-motion of colon carcinogenesis. With casein, the kinetics of protein fermentation with increasing thermolysis time are clearly different from the kinetics of promotion of ACF growth. The formation of the fermentation products was highest when the protein was thermolyzed for one hour, whereas promotion was highest for protein that had been thermolyzed for two or more hours. With soy and egg white, thermolysis increased colonic protein fermentation but did not promote colon carcinogenesis. Thus, although thermolysis of dietary casein increases colonic protein fermentation, products of this fermentation do not appear to be responsible for the promotion of colon carcinogenesis. Indeed, the results suggest that protein fermentation products do not play an important role in colon cancer promotion

Biomimetic direction of arrival estimation for resolving front-back confusions in hearing aids

Sound sources at the same angle in front or behind a two-microphone array (e.g., bilateral hearing aids) produce the same time delay and two estimates for the direction of arrival: A front-back confusion. The auditory system can resolve this issue using head movements. To resolve front-back confusion for hearing-aid algorithms, head movement was measured using an inertial sensor. Successive time-delay estimates between the microphones are shifted clockwise and counterclockwise by the head movement between estimates and aggregated in two histograms. The histogram with the largest peak after multiple estimates predicted the correct hemifield for the source, eliminating the front-back confusions

Seven new species of Australian Pertusaria (Pertusariales, lichenised Ascomycota) from New South Wales

Seven new species, Pertusaria albula, P. dharugensis, P. ochrodigitula, P. pinnaculata, P. placocarpa, P. puttyensis and P. scabrida from New South Wales are reported as new to science

Biomimetic direction of arrival estimation for resolving front-back confusions in hearing aids

Sound sources at the same angle in front or behind a two-microphone array (e.g., bilateral hearing aids) produce the same time delay and two estimates for the direction of arrival: A front-back confusion. The auditory system can resolve this issue using head movements. To resolve front-back confusion for hearing-aid algorithms, head movement was measured using an inertial sensor. Successive time-delay estimates between the microphones are shifted clockwise and counterclockwise by the head movement between estimates and aggregated in two histograms. The histogram with the largest peak after multiple estimates predicted the correct hemifield for the source, eliminating the front-back confusions

Seven new species of Australian Pertusaria (Pertusariales, lichenised Ascomycota) from New South Wales

Seven new species, Pertusaria albula, P. dharugensis, P. ochrodigitula, P. pinnaculata, P. placocarpa, P. puttyensis and P. scabrida from New South Wales are reported as new to science

The effect of increased channel interaction on speech perception with cochlear implants.

Cochlear implants (CIs) are neuroprostheses that partially restore hearing for people with severe-to-profound hearing loss. While CIs can provide good speech perception in quiet listening situations for many, they fail to do so in environments with interfering sounds for most listeners. Previous research suggests that this is due to detrimental interaction effects between CI electrode channels, limiting their function to convey frequency-specific information, but evidence is still scarce. In this study, an experimental manipulation called spectral blurring was used to increase channel interaction in CI listeners using Advanced Bionics devices with HiFocus 1J and MS electrode arrays to directly investigate its causal effect on speech perception. Instead of using a single electrode per channel as in standard CI processing, spectral blurring used up to 6 electrodes per channel simultaneously to increase the overlap between adjacent frequency channels as would occur in cases with severe channel interaction. Results demonstrated that this manipulation significantly degraded CI speech perception in quiet by 15% and speech reception thresholds in babble noise by 5 dB when all channels were blurred by a factor of 6. Importantly, when channel interaction was increased just on a subset of electrodes, speech scores were mostly unaffected and were only significantly degraded when the 5 most apical channels were blurred. These apical channels convey information up to 1 kHz at the apical end of the electrode array and are typically located at angular insertion depths of about 250 up to 500°. These results confirm and extend earlier findings indicating that CI speech perception may not benefit from deactivating individual channels along the array and that efforts should instead be directed towards reducing channel interaction per se and in particular for the most-apical electrodes. Hereby, causal methods such as spectral blurring could be used in future research to control channel interaction effects within listeners for evaluating compensation strategies

The Effect of Free-Field Presentation and Processing Strategy on a Measure of Spectro-Temporal Processing by Cochlear-Implant Listeners

The STRIPES (Spectro-Temporal Ripple for Investigating Processor EffectivenesS) test is a psychophysical test of spectro-temporal resolution developed for cochlear-implant (CI) listeners. Previously, the test has been strictly controlled to minimize the introduction of extraneous, nonspectro-temporal cues. Here, the effect of relaxing many of those controls was investigated to ascertain the generalizability of the STRIPES test. Preemphasis compensation was removed from the STRIPES stimuli, the test was presented over a loudspeaker at a level similar to conversational speech and above the automatic gain control threshold of the CI processor, and listeners were tested using the everyday setting of their clinical devices. There was no significant difference in STRIPES thresholds measured across conditions for the 10 CI listeners tested. One listener obtained higher (better) thresholds when listening with their clinical processor. An analysis of longitudinal results showed excellent test–retest reliability of STRIPES over multiple listening sessions with similar conditions. Overall, the results show that the STRIPES test is robust to extraneous cues, and that thresholds are reliable over time. It is sufficiently robust for use with different processing strategies, free-field presentation, and in nonresearch settings

Development and validation of a spectro-temporal processing test for cochlear-implant listeners.

Psychophysical tests of spectro-temporal resolution may aid the evaluation of methods for improving hearing by cochlear implant (CI) listeners. Here the STRIPES (Spectro-Temporal Ripple for Investigating Processor EffectivenesS) test is described and validated. Like speech, the test requires both spectral and temporal processing to perform well. Listeners discriminate between complexes of sine sweeps which increase or decrease in frequency; difficulty is controlled by changing the stimulus spectro-temporal density. Care was taken to minimize extraneous cues, forcing listeners to perform the task only on the direction of the sweeps. Vocoder simulations with normal hearing listeners showed that the STRIPES test was sensitive to the number of channels and temporal information fidelity. An evaluation with CI listeners compared a standard processing strategy with one having very wide filters, thereby spectrally blurring the stimulus. Psychometric functions were monotonic for both strategies and five of six participants performed better with the standard strategy. An adaptive procedure revealed significant differences, all in favour of the standard strategy, at the individual listener level for six of eight CI listeners. Subsequent measures validated a faster version of the test, and showed that STRIPES could be performed by recently implanted listeners having no experience of psychophysical testing

The Effect of Free-Field Presentation and Processing Strategy on a Measure of Spectro-Temporal Processing by Cochlear-Implant Listeners

The STRIPES (Spectro-Temporal Ripple for Investigating Processor EffectivenesS) test is a psychophysical test of spectro-temporal resolution developed for cochlear-implant (CI) listeners. Previously, the test has been strictly controlled to minimize the introduction of extraneous, nonspectro-temporal cues. Here, the effect of relaxing many of those controls was investigated to ascertain the generalizability of the STRIPES test. Preemphasis compensation was removed from the STRIPES stimuli, the test was presented over a loudspeaker at a level similar to conversational speech and above the automatic gain control threshold of the CI processor, and listeners were tested using the everyday setting of their clinical devices. There was no significant difference in STRIPES thresholds measured across conditions for the 10 CI listeners tested. One listener obtained higher (better) thresholds when listening with their clinical processor. An analysis of longitudinal results showed excellent test–retest reliability of STRIPES over multiple listening sessions with similar conditions. Overall, the results show that the STRIPES test is robust to extraneous cues, and that thresholds are reliable over time. It is sufficiently robust for use with different processing strategies, free-field presentation, and in nonresearch settings