Discovery and validation of a three-gene signature to distinguish COVID-19 and other viral infections in emergency infectious disease presentations: a case-control and observational cohort study

Summary Background Emergency admissions for infection often lack initial diagnostic certainty. COVID-19 has highlighted a need for novel diagnostic approaches to indicate likelihood of viral infection in a pandemic setting. We aimed to derive and validate a blood transcriptional signature to detect viral infections, including COVID-19, among adults with suspected infection who presented to the emergency department. Methods Individuals (aged ≥18 years) presenting with suspected infection to an emergency department at a major teaching hospital in the UK were prospectively recruited as part of the Bioresource in Adult Infectious Diseases (BioAID) discovery cohort. Whole-blood RNA sequencing was done on samples from participants with subsequently confirmed viral, bacterial, or no infection diagnoses. Differentially expressed host genes that met additional filtering criteria were subjected to feature selection to derive the most parsimonious discriminating signature. We validated the signature via RT-qPCR in a prospective validation cohort of participants who presented to an emergency department with undifferentiated fever, and a second case-control validation cohort of emergency department participants with PCR-positive COVID-19 or bacterial infection. We assessed signature performance by calculating the area under receiver operating characteristic curves (AUROCs), sensitivities, and specificities. Findings A three-gene transcript signature, comprising HERC6, IGF1R, and NAGK, was derived from the discovery cohort of 56 participants with bacterial infections and 27 with viral infections. In the validation cohort of 200 participants, the signature differentiated bacterial from viral infections with an AUROC of 0·976 (95% CI 0·919−1·000), sensitivity of 97·3% (85·8−99·9), and specificity of 100% (63·1−100). The AUROC for C-reactive protein (CRP) was 0·833 (0·694−0·944) and for leukocyte count was 0·938 (0·840−0·986). The signature achieved higher net benefit in decision curve analysis than either CRP or leukocyte count for discriminating viral infections from all other infections. In the second validation analysis, which included SARS-CoV-2-positive participants, the signature discriminated 35 bacterial infections from 34 SARS-CoV-2-positive COVID-19 infections with AUROC of 0·953 (0·893−0·992), sensitivity 88·6%, and specificity of 94·1%. Interpretation This novel three-gene signature discriminates viral infections, including COVID-19, from other emergency infection presentations in adults, outperforming both leukocyte count and CRP, thus potentially providing substantial clinical utility in managing acute presentations with infection. Funding National Institute for Health Research, Medical Research Council, Wellcome Trust, and EU-FP7

To be or not to be metal-free: trends and advances in coupling chemistries

Coupling reactions have been part of several extensive studies in order to develop innovative and greener protocols that can generate a wide range of compounds with applications in pharmaceuticals, agrochemicals and biologically active compounds. Metal-free couplings are an important and increasingly trendy field that has attracted a significant deal of interest in recent years, generating a lot of controversy on the issue of whether metal free is really free. Aside from focusing on such a controversial topic itself, this contribution aims to provide a brief introduction on coupling chemistry to point out the transition of this technology from metal-catalyzed to metal-free. This is followed by a range of key selected synthetically useful metal-free processes and a brief commentary on the current debate of whether metal-free reactions are really metal-free and the required experiments for a full understanding of a metal-free coupling process

Effects of vermicomposts produced from cattle manure, food waste and paper waste on the growth and yield of peppers in the field

Summary Commercially processed vermicomposts, produced from food wastes, paper wastes and cattle manure, were applied to 8.25 m 2 field plots, at rates of 10 or 20 t/ha in 1999 and 5 or 10 t/ha in 2000, to evaluate their effects on the growth and yields of peppers (Capsicum annuum) var. King Arthur. The vermicomposts were incorporated into the upper 10 cm of soil and supplemented, based on chemical analyses, with amounts of inorganic NPK fertilizers calculated to equalize initially with the rates of 95-95 NK kg/ha applied to the inorganic fertilizer control plots. Phosphorus was determined to be adequate in soils at the experiment site so was not added. All treatments were replicated four times in a randomized complete block design. The vermicompost applications increased the growth and yields of peppers significantly; including increased leaf areas, plant shoot biomass, marketable fruit weights and decreased yields of non-marketable fruit. Application of vermicomposts to soils increased their microbial biomass and dehydrogenase activity. Humic materials and other plant growth-influencing substances, such as plant growth hormones, produced by microorganisms during vermicomposting, and produced after increased microbial biomass and activity in soils, may have been responsible for the increased pepper growth and yields, independent of nutrient availability

Flow nanocatalysis: Innovative methodologies towards green chemical syntheses

Green chemistry is a chemical philosophy that aims to develop more sustainable, economical and environmental friendly protocols in the industry. It involves concepts of nanocatalysis, flow chemistry and microwave chemistry to design more sustainable chemical processes. This contribution aims to briefly introduce the concepts of flow nano-catalysis and provide an overview of key developments and examples in the field of green chemical processing

Naturally Nano: Magnetically Separable Nanocomposites from Natural Resources for Advanced Catalytic Applications

The present manuscript describes the use of silk cocoons as a structuring agent for the formation of an iron-based active phase for the controlled oxidation of benzyl alcohol. Different samples were prepared using different calcination temperatures. X-ray diffraction and transmission electron microscopy showed a higher proportion of alpha-Fe2O3 phase and a higher global crystallinity at superior calcination temperature. In terms of catalytic activity, the sample treated at 500 °C presented the highest conversion reaching 47%, with selectivity in benzaldehyde of 75.9%

Mechanical pretreatments of lignocellulosic biomass: towards facile and environmentally sound technologies for biofuels production

The transformation of lignocellulosic biomass into biofuels represents an interesting and sustainable alternative to fossil fuel for the near future. However, one still faces some major challenges for the technology to be fully realized including feedstock costs, novel pretreatment processes, production, transportation, and environmental impact of the full chain. The development of new technologies focused to increase the efficiency of cellulose conversion to biofuels determines successful implementation. Mechanical fractionation is an essential step in order to increase final carbohydrate output, appropriate particle sizes and densification, enzymatic accessibility, and bioconversion affectivity without the production of toxic side streams. In this review article, we surveyed a substantial amount of previous work in mechanical fractionation or pretreatments of a variety of lignocellulosic biomasses; these include numerous milling schemes and extrusions, and their impacts on the physical and physicochemical properties of the lignocellulosic matrix (crystallinity, surface area, particle size, etc). We have also compared results with other pure chemical and physicochemical pretreatments in order to show the new aspects and advantages/disadvantages of such an approach. Last, but not least, the effec