Efecto de la suplementación de proteína y energía sobre la producción láctea, densidad, sólidos totales, grasa y nitrógeno ureico en la leche de vacas Brown Swiss en condiciones hipobáricas naturales

This study aimed to determine the effect of the energy / protein ratio of supplementary feed rations (RAS) on the production and characteristics of milk under natural hypobaric conditions (4300 m altitude in Puno, Peru). A total of 24 Brown Swiss cows in first and second third of lactation were distributed in three RAS with three ratios of metabolizable energy / protein (RAS1: 17.0 kcal/g; RAS2: 14.2 kcal/g; RAS3: 12.4 kcal/g) and as a protein source three levels of hydrolysed sheep and alpaca skins – Pioval-2® (0, 3 and 6%, respectively) and three levels of extruded whole soymeal (0, 10 and 25%, respectively). The cows were grazing on alfalfa associated with rye grass and supplemented with oat silage. Daily milk yield (DMY) was recorded for 45 days, as well as density (DE), total solids (TS), fat (FA) and milk urea nitrogen (MUN) at 0, 15, 30 and 45 days. The RAS did not affect the DMY (17.7, 18.8 and 19.0 kg/day) or the DE (1,031, 1,031 and 1,030 g/l, respectively) of the milk. RAS2 increased the TS and FA (13.0%, 4.2%) in relation to RAS1 (12.4%, 3.8%) and RAS3: 12.2%, 3.9%) (p <0.01) in milk, respectively. The decrease of the energy / protein fraction in the rations increased proportionally the MUN (RAS1: 28.9; RAS2: 32.3; RAS3: 36.8 mg/dl; p<0.001). In conclusion, the increase in the energy / protein fraction of the concentrate ration did not affect milk yield or milk density but improved the total solids and fat content; while the reduction of the energy / protein ratio in the ration consistently increased the urea nitrogen in the milk.El estudio tuvo como objetivo determinar el efecto de la ratio energía / proteína de las raciones alimenticias suplementarias (RAS) sobre la producción y características de la leche en condiciones hipobáricas naturales (4300 m de altitud en Puno, Perú). Se distribuyeron al azar 24 vacas Brown Swiss de primer y segundo tercio de lactación en tres RAS con tres ratios de energía metabolizable / proteína (RAS1: 17.0 kcal/g; RAS2: 14.2 kcal/g; RAS3: 12.4 kcal/g) y como fuente de proteína tres niveles de harina hidrolizada de pieles de ovino y alpaca – Pioval-2® (0, 3 y 6%, respectivamente) y tres niveles de harina de soya integral extruida (0, 10 y 25%, respectivamente). Las vacas estaban al pastoreo sobre cultivo de alfalfa asociado a rye grass y recibieron ensilado de avena. Se determinó la producción diaria de leche (PDL) durante 45 días, así como la densidad (DE), sólidos totales (ST), grasa (GR) y nitrógeno ureico en la leche (NUL) a los 0, 15, 30 y 45 días. Las RAS no afectaron la PDL (17.7, 18.8 y 19.0 kg/día) ni la DE (1,031, 1,031 y 1,030 g/l, respectivamente) de la leche. La RAS2 incrementó los ST y GR (13.0%, 4.2%) con relación a RAS1 (12.4%, 3.8%) y RAS3: 12.2%, 3.9%) (p<0.01) en la leche, respectivamente. Con la disminución de la fracción energía / proteína en las raciones el NUL aumentó proporcionalmente (RAS1: 28.9; RAS2: 32.3; RAS3: 36.8 mg/dl; p<0.001). En conclusión, el aumento de la fracción energía / proteína de la ración de concentrado no afectó la producción ni la densidad de la leche, pero mejoró el contenido de sólidos totales y grasa; mientras que la reducción de la ratio energía / proteína en la ración incrementó consistentemente el nitrógeno ureico en la leche

Temperature dependence of anion transport in the human red blood cell

Arrhenius plots of chloride and bromide transport yield two regions with different activation energies (Ea). Below 15 or 25°C (for Cl− and Br−, respectively), Ea is about 32.5 kcal/mol; above these temperatures, about 22.5 kcal/mol (Brahm, J. (1977) J. Gen. Physiol. 70, 283–306). For the temperature dependence of SO42− transport up to 37°C, no such break could be observed. We were able to show that the temperature coefficient for the rate of SO42− transport is higher than that for the rate of denaturation of the band 3 protein (as measured by NMR) or the destruction of the permeability barrier in the red cell membrane. It was possible, therefore, to extend the range of flux measurements up to 60°C and to show that, even for the slowly permeating SO42− in the Arrhenius plot, there appears a break, which is located somewhere between 30 and 37°C and where Ea changes from 32.5 to 24.1 kcal/mol. At the break, the turnover number is approx. 6.9 ions/band 3 per s. Using 35Cl−-NMR (Falke, Pace and Chan (1984) J. Biol. Chem. 259, 6472–6480), we also determined the temperature dependence of Cl−-binding. We found no significant change over the entire range from 0 to 57°C, regardless of whether the measurements were performed in the absence or presence of competing SO42−. We conclude that the enthalpy changes associated with Cl−-or SO42−-binding are negligible as compared to the Ea values observed. It was possible, therefore, to calculate the thermodynamic parameters defined by transition-state theory for the transition of the anion-loaded transport protein to the activated state for Cl−, Br− and SO42− below and above the temperatures at which the breaks in the Arrhenius plots are seen. We found in both regions a high positive activation entropy, resulting in a low free enthalpy of activation. Thus the internal energy required for carrying the complex between anion and transport protein over the rate-limiting energy barrier is largely compensated for by an increase of randomness in the protein and/or its aqueous environment

Roles of histidine 752 and glutamate 699 in the pH dependence of mouse band 3 protein-mediated anion transport

In the accompanying paper we have shown that four different histidine residues are involved in the maintenance of mouse band 3 in a state in which it is able to execute its anion transport function. Here we focus on the functional significance of His 752 and demonstrate that this residue, together with Glu 699, plays a key role in the control of pH dependence of Cl- transport. Mouse band 3-encoding cRNA was expressed in Xenopus oocytes, and band 3-mediated Cl- transport was measured at zero membrane potential over the pH range 6.0-9.2. Transport decreased with increasing H+ concentration and was governed by a single pK of 5.8. After correction for temperature differences, this result agrees well with measurements in erythrocyte ghosts of Cl- flux by Funder and Wieth [Funder, J., & Wieth, J. O. (1976) J. Physiol. 262, 679-698] and our own determinations by 35Cl NMR spectroscopy of Cl- exchange between the substrate binding site and the medium. After mutation of either Glu 699 to Asp or of His 752 to Ser, the maximal rate of transport is reduced and the rate of anion exchange is now governed by a single pK of about 6.8-6.9. This suggests that the formation of a hydrogen bond between His 752 and Glu 699 is essential for the decrease of band 3-mediated Cl- transport at low pH. We suggest that in the wild type band 3 both the decrease of the chloride exchange between the medium and the substrate binding site and the inhibition of chloride translocation across the membrane are dominated by a common rate-limiting step and that this step involves hydrogen bond formation between Glu 699 and His 752

Photonic-Based Integrated Sources and Antenna Arrays for Broadband Wireless Links in Terahertz Communications

This paper analyzes integrated components for ultra-broadband millimeter-wave wireless transmitters enabling the 5 G objective to increase the wireless data rates 10× to 100×. We have pursued the photonic-based approach to generate the millimeter-wave carrier (≈97 GHz in this paper) through photomixing. We have achieved up to 10 Gb s-1 data rate using an OOK modulation format (to reduce latency) and either direct detection (DD) or coherent detection. We show that coherent detection enables a sensitivity improvement of 17 dB over DD. We also demonstrate in this work that such improvement can be achieved using as the transmitter a novel integrated antenna array - the self-complementary chessboard array. This avoids the use of complex coherent schemes at the receiver, enabling simple DD for ultra-broadband links