2 research outputs found
Colibacillosis In Lambs Is Associated To Type I Heat-stable Enterotoxin In A Farm In São Paulo State, Brazil [colibacilose Em Carneiros é Associada à Enterotoxina Termo-estável Do Tipo I Em Uma Propriedade Rural Do Estado De São Paulo, Brasil]
Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was not detected among the diarrheic feces. These data suggests that STa must be an important diarrheagenic factor to small ruminants in São Paulo State.425854857Bertin, Y., Rapid and specific detection of F17-related pilin and adhesion genes in diarrheic and septicemic Escherichia coli strains by multiplex PCR (1996) Journal of Clinical Microbiology, 34, pp. 2921-2928Beutin, Y., Burgos, L., Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli (2010) BMC. Microbiology, 10, p. 193. , http://www.biomedcentral.com/1471-2180/10/193, Available from, Accessed: Jul. 29, 2011. doi. 10.1186/1471-2180-10-193Blanco, J., Serogroups of Escherichia coli strains producing cytotoxic necrotizing factors CNF1 and CNF2 (1992) FEMS Microbiology Letters, 96, pp. 155-159. , http://www.sciencedirect.com/science/article/pii/0378109792903966, Available from, Accessed: Jan. 22, 2012. doi. 10.1016/0378-1097(92)90396-6Blanco, M., Genes coding for Shiga-like toxins in bovine Verotoxin-producing Escherichia coli (VTEC) strains belonging to different O:K:H serotypes (1994) Veterinary Microbiology, 42, pp. 105-110. , http://www.sciencedirect.com/science/article/pii/0378113594900108, Available from, Accessed: Jul. 29, 2011. doi. 10.1016/0378-1135(94)90010-8Blanco, J., Serogroups, toxins and antibiotic resistance of Escherichia coli strains isolated from diarrhoeic lambs in Spain (1996) Veterinary Microbiology, 49, pp. 209-217. , http://www.sciencedirect.com/science/article/pii/0378113595001883, Available from, Accessed: Jul. 29, 2011. doi. 10.1016/0378-1135(95)00188-3Blanco, M., Serotypes, virulence genes, and intimin types of Shiga toxin (Verotoxin)-producing Escherichia coli isolates from healthy sheep in Spain (2003) Journal of Clinical Microbiology, 41, pp. 1351-1356. , http://jcm.asm.org/cgi/reprint/41/4/1351, Available from, Accessed: Jul. 29, 2011. doi. 10.1128/JCM.41.4.1351-1356.200Cid, D., F17 fimbriae in Escherichia coli from lambs and kids (1993) Veterinary Research, 132, p. 251Dean, A.G., Test for Escherichia coli enterotoxin using infant mice: Application in a study of diarrhea in children in Honolulu (1972) Journal of Infectious Diseases, 124, pp. 407-411. , http://jid.oxfordjournals.org/content/125/4/407.short, Available from, Accessed: Jul. 26, 2011. doi. 10.1093/infdis/125.4.40Fidock, D.A., Nucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41 (1989) Nucleic Acids Research, 17, p. 2849Herring, A.J., Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver stained polyacrylamide gels (1982) Journal of Clinical Microbiology, 16, pp. 473-477. , http://jcm.asm.org/cgi/reprint/16/3/473, Available from, Accessed: Jul. 27, 2011Roosendaal, B., The nucleotide sequence of the gene encoding the K99 subunit of enterotoxigenic Escherichia coli (1984) FEMS Microbiology Letters, 22, pp. 253-258Smith, H.W., Huggins, M.B., Effectiveness of phages in treating experimental Escherichia coli diarrhoea in calves, piglets and lambs (1983) Journal of General Microbiology, 129, pp. 2659-2675. , http://mic.sgmjournals.org/content/129/8/2659.full.pdf+html, Available from, Accessed: Jul. 26, 2011. doi.10.1099/00221287-129-8-2659Theil, K.W., Rotaviruses associated with neonatal lamb diarrhea in two wyoming shed-lambing operations (1996) Journal of Veterinary Diagnostic Investigation, 8, pp. 245-248. , http://vdi.sagepub.com/content/8/2/245.full.pdf, Available from, Accessed: Jul. 27, 2011. doi. 10.1177/104063879600800217Toledo, M.R., MILi - um meio para a realizacao dos testes de motilidade, indol e lisina descarboxilase (1982) Revista De Microbiologia, 13, pp. 230-235Toledo, M.R., EPM - Modificação do meio de Rugai e Araujo para a realização simultânea dos testes de produção de gás a partir da glicose, H2S, urease e triptofano desaminase (1982) Revista De Microbiologia, 13, pp. 309-315Urdahl, A.M., Serotypes and virulence factors of Shiga toxin-producing Escherichia coli isolated from healthy Norwegian sheep (2002) Journal of Applied Microbiology, 93, pp. 1026-1033. , http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2002.01787.x/pdf, Available from, Accessed: Jul. 26, 2011. doi. 10.1046/j.1365-2672.2002.01787.xVettorato, M.P., Properties of Shiga toxin-producing Escherichia coli (STEC) isolates from sheep in the State of São Paulo, Brazil (2003) Veterinary Microbiology, 95, pp. 103-109. , http://www.sciencedirect.com/science/article/pii/S0378113503001536, Available from, Accessed: Jul. 26, 2011. doi. 10.1016/S0378-1135(03)00153-6Yamamoto, S., Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction (1995) FEMS Immunology and Medical Microbiology, 12, pp. 85-90. , http://www.sciencedirect.com/science/article/pii/092882449500053A, Available from, Accessed: Jul. 29, 2011. doi. 10.1016/0928-8244(95)00053-
Cellulitis Lesions In Broiler Chickens Are Induced By Escherichia Coli Vacuolating Factor (ecvf)
Escherichia coli Vacuolating Factor (ECVF) is a heat-labile, vacuolating cytotoxin produced by avian pathogenic E. coli (APEC) isolated from avian cellulitis lesions. In this report, we intend to demonstrate that purified ECVF induces the inflammatory process of cellulitis. Our group is the first to demonstrate the effect of ECVF in a histological analysis by in situ inoculation of broiler chickens with purified ECVF. The animals were inoculated with the APEC AC53 and with purified ECVF subcutaneously on their ventral surface (in the sternum region). The histological analysis showed different grades of an acute inflammatory response in the epidermis, dermis and panniculus. An increase in mRNA expression of the proinflammatory cytokine TNF-α was also demonstrated in the inflamed tissue. When ECVF was systemically administered, increased levels of TNF-α and IL-10 were observed in the serum. These results suggest that ECVF plays a key role in the inflammatory process associated with cellulitis that is mainly mediated by TNF-αIn addition, this inflammation can be downregulated by the anti-inflammatory cytokine IL-10. © 2012.1622/Abr866872Behmer, O.A., Tolosa, E.M.C., Freitas Neto, A.G., (1976) Manual de Técnicas para Histologia Normal e Patológica, , Edart, São Paulo, p. 315Carr, D., Shaw, D., Halvorson, D.A., Rings, B., Roepke, D., Excessive mortality in market-age turkeys associated cellulites (1996) Avian Dis., 40, pp. 736-741Dutta, P.R., Cappello, R., Navarro-GarcÃa, F., Nataro, J.P., Functional comparison of serine protease autotransporters of enterobacteriaceae (2002) Infect. Immun., 70, pp. 7105-7113Ewers, C., Janben, T., Kiebling, S., Philipp, H.C., Wieler, L.H., Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry (2004) Vet. Microbiol., 104, pp. 91-101Fallavena, L.C.B., Moraes, H.L.S., Salle, C.T.P., Silva, A.B., Vargas, R.S., Nascimento, V.P., Canal, C.W., Diagnosis of skinlesions in condemned or downgraded broiler carcasses - a microscopic and macroscopic study (2000) Avian Pathol., 29, pp. 557-562Gerard, C., Bruyns, C., Marchand, A., Abramo wicz, D., Vandenabeele, P., Delvaux, A., Fiers, V., Velu, M., Interleukin-10 reduces the release of tumor necrosis factor and prevents lethality in experimental endotoxemia (1993) J. Exp. Med., 177, pp. 547-550Gomis, S.M., Watts, T., Riddell, C., Potter, A.A., Allan, B.J., Experimental reproduction of Escherichia coli cellulitis and septicemia in broiler chickens (1997) Avian Dis., 41, pp. 234-240Henderson, I.R., Navarro-Garcia, F., Desvaux, M., Fernandez, R., Ala'Aldeen, D., Type V protein secretion pathway: the autotransporter story (2004) Microbiol. Mol. Biol. Rev., 68, pp. 692-744Jeffrey, J.S., Chin, R.P., Singer, R.S., Assessing cellulitis pathogenicity of Escherichia coli isolates in broiler chickens assessed by an in vivo inoculation model (1999) Avian Dis., 43, pp. 491-496Kostakioti, M., Stathopoulos, C., Functional analysis of the Tsh autotransporter from an avian pathogenic Escherichia coli strain (2004) Infect. Immun., 72, pp. 5548-5554Laster, S.M., Wood, J.G., Gooding, L.R., Tumor necrosis factor can induce both apoptotic and necrotic form of cell lysis (1988) J. Immun., 141, pp. 2629-2635Malefyt, R., Abrams, J., Bennett, B., Figdor, C.G., De Vries, J.E., Interleukin 10 (IL 10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL10 produced by monocytes (1989) J. Exp. Med., 17, pp. 1209-1220Messier, S., Quessy, S., Robinson, Y., Devriese, L.A., Hommez, J., Fairbrother, J.M., Focal dermatitis and cellulitis in broiler chickens: bacteriological and pathological findings (1993) Avian Dis., 37, pp. 839-844Moore, K.W., OuGarra, A., Malefyt, R.W., Vieira, P., Mosmann, T.R., Interleukin-10 (1993) Annu. Rev. Immunol., 11, pp. 165-190Ngeleka, M., Kwaga, J.K.P., White, D.G., Whittam, T.S., Riddell, C., Goodhope, R., Potter, A.A., Allan, B., Escherichia coli cellulitis in broiler chickens: clonal relationships among strains and analysis of virulence-associated factors of isolates from diseased birds (1996) Infect. Immun., 64, pp. 3118-3126Norton, R.A., Bilgili, S.F., McMurtrey, B.L., A reproducible model for the induction of avian cellulitis in broiler chickens (1997) Avian Dis., 41, pp. 422-428Onderka, D.K., Hanson, J.A., McMillan, K.R., Allan, B., Escherichia coli associated cellulitis in broilers: correlation with systemic infection and microscopic visceral lesions, and evaluation for skin trimming (1997) Avian Dis., 41, pp. 935-940Pajkrt, D., Camoglio, L., Tiel-Van Buul, M.C., de Bruin, K., Cutler, D.L., Affrime, M.B., Rikken, G., van Deventer, S.J., Attenuation of proinflammatory response by recombinant human IL 10 in human endotoxemia: effect of timing of recombinant human IL-10 administration (1997) J. Immun., 158, pp. 3971-3977Parham, N.J., Pollard, S.J., Desvaux, M., Scott-Tucker, A., Liu, C., Fivian, A., Henderson, I.R., Distributions of the serine protease autotransporters of the Enterobacteriaceae among extraintestinal clinical isolates of Escherichia coli (2005) J. Clin. Microbiol., 43, pp. 4076-4082Parreira, V.R., Gyles, C.L., A novel pathogenicity island integrated adjacent to the thrW tRNA gene of avian pathogenic Escherichia coli encodes a vacuolating autotransporter toxin (2003) Infect. Immun., 71, pp. 5087-5096Peighambari, S.M., Julian, R.J., Vaillancourt, J.P., Gyles, C.L., Escherichia coli cellulitis: experimental infections in broiler chickens (1995) Avian Dis., 39, pp. 125-134Randall, C.J., Meakins, P.A., Watt, D.J., A new skin disease in broilers (1984) Vet. Rec., pp. 114-246Salvadori, M.R., Yano, T., Carvalho, H.F., Parreira, V.R., Gyles, C.L., Vacuolating cytotoxin produced by avian pathogenic Escherichia coli (2001) Avian Dis., 45, pp. 43-51Standiford, T.J., Strieter, R.M., Lukacs, N.W., Kunkel, S.L., Neutralization of IL-10 increases lethality in endotoxemia. Cooperative effects of macrophage inflammatory protein 2 and tumor necrosis factor (1995) J. Immun., 155, pp. 2222-2229Weinrauch, Y., Zychlinsky, A., The induction of apoptosis by bacterial pathogens (1999) Annu. Rev. Microbiol., 53, pp. 155-187Zhou, G., Cai, W., Liu, X., Niu, C., Gao, C., Si, C., Zang, W., Han, L., A duplex real-time reverse transcription polymerase chain reaction for the detection and quantitation of avian leukosis virus subgroups A and B (2011) J. Virol. Methods, 173, pp. 275-27