<i>L</i>. <i>pneumophila</i> sg. 1 growth in axenic conditions and in co-culture with the two <i>Acanthamoeba</i> strains in PYG liquid media.

<p>Samples were taken at different time points and plated on BCYE agar plates. Data are presented as means ± SD (error bars; n = 3).</p

Effect of free chlorine 0.2 mg L^-1 and 0.5 mg L^-1, on the inactivation of 5 <i>Legionella</i> strains.

<p>Bacterial inactivation was determined using viable counts on BCYE agar medium. Data are presented as means ± SD (columns and error bars; n = 3). Statistical differences between means within each time point were represented assigning different letters to the bar plot. The same letter was assigned to bars with no significant differences between them. Statistical analyses were performed by ANOVA and pairwise Fisher’s LSD test (<i>p</i><0.05).</p

Effect of free chlorine, 1.2 mg L^-1 and 2.5 mg L^-1, on the inactivation of 2 <i>Acanthamoeba</i> strains treating separately trophozoites and cysts.

<p>Amoebal inactivation was determined using an adaptation of the MPN method. Data are presented as means ± SD (columns and error bars; n = 3). Statistical differences between means within each time point were represented assigning different letters to the bar plot. The same letter was assigned to bars with no significant differences between them. Statistical analyses were performed by ANOVA and pairwise Fisher’s LSD test (<i>p</i><0.05).</p

Effect of thermal treatments on the inactivation of 5 <i>Legionella</i> strains.

<p>Bacterial inactivation was determined using viable counts on BCYE agar medium. Data are presented as means ± SD (columns and error bars; n = 3). Statistical differences between means within each time point were represented assigning different letters to the bar plot. The same letter was assigned to bars with no significant differences between them. Statistical analyses were performed by ANOVA and pairwise Fisher’s LSD test (<i>p</i><0.05).</p

Effect of free chlorine, on the inactivation of <i>L</i>. <i>pneumophila</i> sg. 1 env. associated to two <i>Acanthamoeba</i> strains, <i>A</i>.<i>castellanii</i> CCAP 1534/2 and <i>Acanthamoeba</i> sp. 155 strains.

<p>Bacterial inactivation was determined using viable counts on BCYE medium. Data are presented as means ± SD (columns and error bars; n = 3). Statistical differences between means within each time point were represented assigning different letters to the bar plot. The same letter was assigned to bars with no significant differences between them. Statistical analyses were performed by ANOVA and pairwise Fisher’s LSD test (<i>p</i><0.05).</p

Effect of thermal treatments on the inactivation of <i>L</i>. <i>pneumophila</i> sg. 1 env. associated to two <i>Acanthamoeba</i> strains, <i>A</i>.<i>castellanii</i> CCAP 1534/2 and <i>Acanthamoeba</i> sp. 155 strains.

<p>Bacterial inactivation was determined using viable counts on BCYE medium. Data are presented as means ± SD (columns and error bars; n = 3). Statistical differences between means within each time point were represented assigning different letters to the bar plot. The same letter was assigned to bars with no significant differences between them. Statistical analyses were performed by ANOVA and pairwise Fisher’s LSD test (<i>p</i><0.05).</p