Transgene inheritances and genetic similarities of near isogenic lines of genetically modifi ed common beans

O objetivo do presente trabalho foi determinar a herança e a estabilidade de transgenes de uma linhagem de feijoeiro-comum com expressão dos genes rep-trap-ren, do Bean golden mosaic virus, e do gene bar. Foram realizados cruzamentos entre a linhagem transgênica e quatro cultivares comerciais de feijão, seguidos de quatro retrocruzamentos. As progênies de cada cruzamento foram avaliadas quanto à presença dos transgenes, com aplicação do glifosinato de amônia nas folhas e por meio da reação da polimerase em cadeia com uso de oligonucleotídeos específicos. O vírus do mosaico comum necrótico do feijoeiro, Bean common mosaic necrosis virus (BCMNV), foi inoculado mecanicamente nas gerações avançadas. Os transgenes foram herdados em padrão mendeliano nos quatro cruzamentos estudados. As linhagens analisadas apresentaram cerca de 80% das características do parental recorrente, conforme determinado por análises com uso de marcadores de DNA, além de manter caracteres importantes, tais como resistência ao BCMNV. A presença do transgene não causou efeitos indesejáveis que pudessem ser detectados nas progênies avaliadas.The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specific oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close to 80% of the characteristics of the recurrent parent, as determined by the random amplified DNA markers used, besides maintaining important traits such as resistance to BCMNV. The presence of the transgene did not cause any detectable undesirable effect in the evaluated progenies

Variability of ricin content in mature seeds of castor bean

O objetivo deste trabalho foi avaliar a concentração de ricina em sementes de mamona (Ricinus communis L.) de 20 acessos do Banco de Germoplasma de Mamoneira da Embrapa Algodão, por meio de ensaio imunoabsorvente de ligação de enzimas. Foram observadas diferenças significativas entre os acessos. O BRA 3271 apresentou a maior concentração de ricina nas sementes (32,18 ng mg-1), e o BRS Paraguaçu a menor (3,53 ng mg-1). Há possibilidade de seleção de genótipos com diferentes concentrações de ricina, que poderão ser utilizados dependendo do interesse dos programas de melhoramento.The objective of this work was to evaluate ricin concentration in castor bean seeds (Ricinus communis) of 20 accessions from the Banco de Germoplasma de Mamoneira of the Embrapa Algodão, Campina Grande, PB, Brazil, using the Enzyme Linked Immunosorbent Assay. Significant differences were observed among accessions. BRA 3271 had the highest ricin concentration in seeds (32.18 ng mg-1), and BRS Paraguaçu had the lowest (3.53 ng mg-1). There is the possibility of selecting genotypes with different ricin concentrations, which can be used according on the interest of the breeding programs

Vitamin D3 Supplementation: An Option Associated with The Treatment of Multiple Sclerosis: : A Systematic Review and Meta-Analysis

Multiple sclerosis (MS) is a chronic complex neurodegenerative disease. A systematic review and meta-analysis were conducted on observational studies and analytics on impact of Vitamin D supplementation in patients with Multiple Sclerosis. In our research, a total of 457 articles were selected and identified for analysis. This systematic review article and meta-analysis, which included evidence from randomized controlled trials conducted with patients with multiple sclerosis, revealed that Vitamin D3 supplementation is effective as an option associated with the treatment of this disease, and that it also has a diffuse protective role against various remission outbreaks in the health. Doses (50,000 IU/week) are appropriate to restore neuroimmunological parameters when used within 12 weeks

The Use of Vitamin D in The Infectious Process in The Hospital Period in Childhood Effective?

Goals: To verify whether the use of Vitamin D as parallel therapy to hospital and drug treatment can be effective in the process of infectious reduction in hospitalized children. Data source: This study is a systematic review and meta-analysis of randomized controlled trials, published between 2011 and the first quarter of 2019, in the Cochrane Library, Medline, US National Library of Medicine and the National Institute of Health (PubMed), Literature databases. Latin American and Caribbean Health Sciences (Lilacs), Scopus and Web of Science. The studies were scored by the Down and Black scale associated with the quality assessment method according to the Cochrane criteria (RCT). Summary of the data: Of the 1475 studies, 09 were included. There is a direct relationship between Vitamin D level and mortality rate in hospitalized children with infections. Conclusion: This study highlighted that the vitamin D deficiency in children under serum analysis during hospitalization triggers severe immunological changes

Gene flow in transgenic soybean in the Cerrado region, Brazil

O objetivo deste trabalho foi avaliar a dispersão de pólen transgênico em soja. Plantas transgênicas de soja contendo os genes ahas, para tolerância ao herbicida imazapyr, e uidA (GUS), foram cultivadas com plantas não-transgênicas. A dispersão do pólen transgênico foi avaliada pela presença de ambos os genes dominantes na progênie de plantas não-transgênicas. A maior freqüência de disseminação de pólen transgênico foi observada na primeira linha, distante 0,5 m da parcela central (0,44% a 0,45%). Esta freqüência foi reduzida drasticamente na linha 2 (0,04% a 0,14%), atingindo 0 na linha 13, a 6,5 m da parcela central.The objective of this work was to evaluate pollen dispersal from transgenic to non-transgenic soybean plants. Non-transgenic soybean plants were cultivated surrounding transgenic soybean plants carrying the ahas (for herbicide tolerance against imazapyr) and gus genes. Pollen dispersal was evaluated by the presence of both dominant genes in the progenies of non-transgenic plants. The highest amount of transgenic pollen dissemination was observed in the first row, 0.5m distant from the central plot (0.44% to 0.45%). The frequency of pollen dispersion has drastically decreased in the row 2 (0.04% to 0.14%), reaching zero by the row 13, which was 6.5 m distant from the central plot

Clinical Epidemiological Profile of Children with Acute Respiratory Insuficiency in a Pediatric Intensive Care Unit

Objective: to know the epidemiological clinical profile of children admitted for acute respiratory failure (ARF) in the Pediatric Intensive Care Unit (ICU) of the Teresina Emergency Hospital (TEH), from 2010 to 2015. Method: a quantitative approach, with a sample of 451 medical records obtained in the electronic system of hospitalization of the patients who were admitted to the Pediatric ICU of the Hospital. After approval by the Ethics Committee of Estácio Ceut Faculty, the study was started. Results: Of the 721 hospitalizations due to ARF in children, 451 (62.6%) required clinical interventions in the ICU. Of these, 59.4% were male, in the age range of one to six years (61.9%), with a diagnosis in the ICD 10 by J96.0. In 99.7% of the cases, hospitalized for a period of more than ten days (72.4%), originating in the interior of Piauí (63.4%), whose discharge occurred by administrative closure (75.6%), followed by deaths, which were 19,3%. Conclusion: Acute Respiratory Insufficiency is a serious problem of global, national and local public health, because it is a morbidity that requires advanced technology for health treatment, with relative mortality. It is reasonable for therapeutic measures to prevent respiratory diseases to be systematically stimulated and implemented by health services to prevent them. KEYWORDS: Infant Mortality; Respiratory Insufficiency; Epidemiology