ELISA inhibition studies in ORA+ subjects.

<p>Bromelain (CCD, solid line) and obeche (dotted line) are inhibitors and obeche extract is the solid phase.</p

Measurement of specific antibodies to obeche wood dust.

<p>Means are represented by dotted lines and a horizontal line indicates cut-off value. *p<0.05 symptomatic vs asymptomatics; **p<0.05 symptomatic vs controls. <b>1A</b>. Levels of specific IgE in ORA+, ORA− and CG subjects; <b>1B</b>. Levels of specific IgG in ORA+, ORA− and CG subjects.</p

Results of <i>in vivo</i> and <i>in vitro</i> tests with obeche extract and purified proteins in subjects with confirmed occupational rhinitis and asthma (ORA+; R = rhinitis; R+A = rhinitis and asthma).

<p>Results regarding food allergy assessment and CCD recognition are also included.</p

Demographic and clinical characteristics of subjects with confirmed occupational rhinitis/asthma (ORA+) <i>vs.</i> asymptomatic exposed (ORA−) and controls (CG). SPT: skin prick test; SD: standard deviation; *: p<0.05.

<p>Demographic and clinical characteristics of subjects with confirmed occupational rhinitis/asthma (ORA+) <i>vs.</i> asymptomatic exposed (ORA−) and controls (CG). SPT: skin prick test; SD: standard deviation; *: p<0.05.</p

Allergenic activity of purified proteins of obeche wood dust.

<p><b>4A</b>. IgE-binding (ELISA) to purified obeche proteins (24 and 12 kDa). Solid line shows the positivity cut-off point, dotted lines show mean OD for each group. <b>4B</b>. Basophil activation tests using the <i>in-house</i> obeche wood dust extract and purified proteins (24 and 12 kDa). The solid line shows the threshold of positivity.</p

Lipid transfer proteins (LTP) included in the homemade array were separated by SDS-PAGE and stained with Coomassie Blue.

<p>Replicas were electrotransferred and incubated with polyclonal antibodies produced against peach LTP (dilution 1∶500). The name of the proteins corresponds to Table I.</p

Purified proteins included in the LTP microarray.

<p>r recombinant protein;</p>*<p>non-LTPs or control proteins included in the array are marked with an asterisk.</p

Average weight of allergens included in the co-sensitization graph.

<p>Average weight of allergens included in the co-sensitization graph.</p

Recognition frequencies of food and pollen LTPs by geographical area.

<p>The recognition frequencies, shown as percentage of positive response (%), were obtained incubating the LTP microarray with single sera from allergic patients. Only LTPs with a positive response of more than 20% (taking all patients into account) are represented. Percentage positive responses and significant differences (p<0.05) are indicated.</p

Frequency of LTP sensitization.

<p><b>A</b>. Recognition frequencies of food and pollen LTPs comparing fruit allergic patients with and without pollen sensitization (Pollen Fruit Allergy and Fruit Allergy, respectively). The recognition frequencies, shown as percentage of positive response (%), were obtained incubating the LTP microarray with single sera from allergic patients. <b>B</b>. Analysis of LTP crossreactivities by inhibition assays using the LTP microarray as solid phase and Pru p 3, Art v 3 and Cas s 8 (5, 1, 0.1, 0.01 µg/mL) as inhibitors. The inhibition percentage of the IgE binding capacity is indicated. Means (n = 3) and SDs (bars) are represented. All tests were performed in triplicate.</p